Our Genomic Unit offers services in the seven areas listed below:
The laser microdissection systems uses a laser to isolate specific microscopic regions from tissue samples. Researcher can selectively and routinely analyze regions of interest down to sigle cells from all kind of tissues, even living cells from cell culture, to obtein results that are relevant, reproducible, and specific.
A ergonomic high-end cryostat with a rotatory microtome that use a motorised µm advance/return. Section thickness setting range from 0.5 to 100 µm and a temperature range from -10ºC to -45ºC. The system includes a spaceous stainless steel chamber and 24 easily accessible freezing positions.
Assessing the quantity and quality of your experimental starting material is essential for research success. The Agilent 2100 Bioanalyzer system is a versatile tool for sample quality control (QC) in many workflows, including next generation sequencing, gene expression, and proteomics. It allows evaluation of integrity, concentration and purity of RNA, DNA and protein samples derived from a large range of origins, including FFPE samples, laser-microdissected tissues, and biopsies. It can also perform fluorescent analysis of intact cells of limited quantity.
The HydroShear offering a simple and easy-to-use, reproducible, and controllable method of fragmenting DNA. The HydroShear DNA Shearing creates and controls hydrodynamic forces that work in conjunction with its innovative shearing assembly to shear DNA. A wide range of DNA samples are compatible with the HydroShear, and the shearing parameters can be adjusted to produce specific fragment lengths of DNA.
The system is ideal for uniform and reproducible making of large DNA fragments for long reads with Next- and 3rd High Throughput Generation Sequencers, and is the best system to generate large DNA fragments needed for long sequencing reads and genomic library construction (example Genomic and Paired End Library Construction).
CHEF-DR® III variable angle system combines patented PACE technology and proven CHEF technology into one easy-to-use instrument that lets you use up to three blocks of run conditions to generate high-resolution separations; specify any electrophoresis angle from 90 to 120°.
Separation of chromosome-sized DNA can be achieved in almost half the time previously possible, without sacrificing resolution.
Based on the detection of target-specific fluorescence, this fluorometers system (Qubit® 3.0 and Quantifluor Fluorometers) are more sensitive than UV absorbance–based quantification, making it ideal for precious samples and demanding applications.
The Elutrap System is designed to isolate nucleic acids and proteins from agarose or polyacrylamide gel slices by electroelution. Samples are purified with excellent recovery into volumes as low as 200 μl, without requiring sample pretreatment or special buffers. The Elutrap Electrophoresis Chamber allows for the most efficient flow of current through the device and can be used for up to four samples simultaneously.
