CHEF System

 Chef DRIII (Biorad)

The pulsed field gel electrophoresis is used for analysis and identification of DNA fragments larger than 2K. The resolution ranges from 100 bp DNAs. to 10 Mb.This electrophoretic technique allows to solve DNA sizes of the order of chromosomes. The separation is performed by alternating electric field between different pairs of electrodes causing a continuous fragments´s reorientation that  migrate through the agarose. In this way it can perform the fractionation of high molecular weight DNA.