SustainPineDB

Assembly Name: uma v2.0

UniGene Name: uma_v2.0_unigene633

Length: 2140 nt

UniGene Fasta

>uma_v2.0_unigene633
TTCCTCCTAAATGTGTCGAAGGTATTTGGTGTGGCAGGCTTCATTGCTGTTGCGGCCAACACCTTTGCCTTTTTTCGAGTTCACAGAAGGAAGTTGGCTCCCTTTGAAAATCCAATTGATGAGTCACAGGAGGTGCTCGCAAGCTTTCCAGTAAACAAAGATGTGGATGACGACGGCTTTTTCTTTGCCATTTCAACTGCTTCTGCTCTTCTAGAAGAAGATGCATGTCTTAAGTTTGCAGCCGAGTATGGTGATTATCCGAAGGAGATAAAACCAGAACCCAAAGATGGTAACCAATCGATTTTGTCTGAAGCACAAACTGATTTGCAAGTCAGAAGTAACCAGCAATTAGGCGAAGACAGAGAGAGTAAAGATGGATATGGTGGTCAAAACTTGGAAATTTCATCAACACTATCCGCAAAAAATCTATCAGAACACCATCAAATCAAGGAAGGTGGACGACAGAATAAGGATTTCTATGAAATGCCATTGGAGGAGTGGGAACTTTTAAATTATGAGCAAATGGCAGAAAGAGAACAGTGGTCTCGAGAACAAGTTTCCAGATTTCCTTCTGTAGCCGGCCATGGCAAATCAGAGGTAGAAAACATAGATTTGGATGATCCAAAAGACCTGCATAAATGTTCTGATCAGCATCTTGAACTTCATGGTAGTATTGCGGATAAGGGAGAGAAACTGTTGGATTCAACTCCTAAAGGGTTGATGAAGGGATCGGAAAATGTTACAGATGAACAGAAAGGAGTGGCTTCTCAGCTAAAACTTACTTGTCTAGAGGAAAAGCCTTGCTCCTGGTCTGATCCAGACCGAGAGCTGCATTTAGCTAAAGAAACGAATGCTACAGTTTTTCGCATGAGTATTGATTGGAACATAATAATGCCAGAAGAACCCCTTGATGGTTTTGAGAAGACTATCAATAGAACAGCACTAGATCGATACAGATGCAAAATTGAAAGAGTTCGTGCGTATGGATTGCGGGTGATGCTGACTCTTTTCCATCAATCTCTACCTAAATGGGCGTGTGCTTATGGTGGTTGGATGGAGGACAAAACTGTTGATTACTTTTTACAATATACAAGGCTAATGGTAGACAGTTTCTATGACCTGGTGGATTACTGGATAACATTCAATGAGCCACATTTATTTGCTTTGACCGAATATTGTTGTGGTGTCCCAGCCAACCTATTCCAAGTTGCTGCAACTGCTTTACATCAAAGAGTTTACAGAAGCACAATGGACAGAATCTTAACAGCTCATATAAAAAGCTTATGATATTATACATGGTACAGGAGAAAAGGCATCAAGAAAAGTAAGTGTTGGCATTGCACATCATACTTCTTTCATGCGACCATATGGCTTCTTTGATATTACTACAGTCGAGTTCAGCAATTGGATTGCATGCTTTGGCTATGTAGATCATGTCTTCAGCAAGCTGGACTTCTTAGGAATAAACTACCATGGGCAGGAATTCATTTCATCTGCTGGGTTGAAACTAGTTGAGAACGAGGAATACAGTGAATCAGGAAGATGCATATACCCAGATGGATTATATCGACTACTCCTTGACTTCCACAACCGCTATAAAATTTTTAATGTTCCTTTCATCATTACTGAAAATGGTGTTGCTGATGCCACTGATCTCCTTCGGAAGCCATATATTCTGGAACATCTCCTTGCTGTAAAAGCAGCCATGAATAAGGGTGTACCTGTTAAAGGTTACTGCTTTTGGGCTTTGTCAGACAACTGGGAATCTGAAAATGAGCATAGCTCCAAGTTTGGCCTTGCTGCTATAGACTCTGATAACAACCTGCAACATATTACTCGACCATCCTATTATCTCTTCTCTGAGGTTGCTAAATCTGGATCAATAACCAAGGGACAAAGGAATAAAGCTTGGGCTGAACTGCAAACTGCAATAGAGCAAGGGAACAACCACCTCATTTGCTTTGGGCCAGACGCTCAAGGTGGGTTGGAGAACCGAGTACAAAGGCCATTTGTTAAAAAGGATTGGAGGTTCGGTCACTATAGACCAGATGGATTACAAGATCCAATGAGCCGTACTATGAGATGGATATGCAGGGCACCGCACATGATATCCACAAGGCAAGAGAGATCGTAACTAAAG

Ace file of the UniGene uma_v2.0_unigene633

Annotations

Parent Assembly	Parent UniGene	Reads (related/total)
SustainPine v2.0	sp_v2.0_unigene26859	30/30

Source	Descriptions	Term	Type	Identity
AutoFact	Putative beta-glycosidase n=1 Tax=Pinus taeda RepID=Q1M317_PINTA	-	-	52%
FL-Next	tr=Putative beta-glycosidase; Pinus taeda (Loblolly pine).	-	-	46%
Blast2go	beta-glucosidase-like chloroplastic-like	-	-	66%

Source	GOs	Term	Type	Identity
Blast2go	response to freezing	GO:0050826	Biological Process	66%
Blast2go	cellobiose glucosidase activity	GO:0080079	Molecular Function	66%
Blast2go	chloroplast outer membrane	GO:0009707	Cellular Component	66%

Source	InterPros	Term	Type	Identity
Sma3	Glycoside hydrolase, family 1	IPR001360	-	-
Sma3	Proteinase inhibitor I3, Kunitz legume	IPR002160	-	-
Sma3	Glycoside hydrolase, subgroup, catalytic domain	IPR013781	-	-
Sma3	Glycoside hydrolase, family 1, active site	IPR018120	-	-

Full-Lengther Next Prediction

Fln status: Putative N-terminus

Fln database: coniferopsida.fasta

Fln subject: Q1M317

Fln msg: Overlapping hits, possible frame ERROR between 1281 and 1277, Unexpected STOP codon at 3' end. Distance to subject end: 38 aas, atg_distance in limit (1-15): atg_distance = 8, W2: There is no M at the beginning, your sequence is longer than subject: 712 - 666

Fln protein: FLLNVSKVFGVAGFIAVAANTFAFFRVHRRKLAPFENPIDESQEVLASFPVNKDVDDDGFFFAISTASALLEEDACLKFAAEYGDYPKEIKPEPKDGNQSILSEAQTDLQVRSNQQLGEDRESKDGYGGQNLEISSTLSAKNLSEHHQIKEGGRQNKDFYEMPLEEWELLNYEQMAEREQWSREQVSRFPSVAGHGKSEVENIDLDDPKDLHKCSDQHLELHGSIADKGEKLLDSTPKGLMKGSENVTDEQKGVASQLKLTCLEEKPCSWSDPDRELHLAKETNATVFRMSIDWNIIMPEEPLDGFEKTINRTALDRYRCKIERVRAYGLRVMLTLFHQSLPKWACAYGGWMEDKTVDYFLQYTRLMVDSFYDLVDYWITFNEPHLFALTEYCCGVPANLFQVAATALHQRVYRSTMDRILTAHIxxxYDIIHGTGEKASRKVSVGIAHHTSFMRPYGFFDITTVEFSNWIACFGYVDHVFSKLDFLGINYHGQEFISSAGLKLVENEEYSESGRCIYPDGLYRLLLDFHNRYKIFNVPFIITENGVADATDLLRKPYILEHLLAVKAAMNKGVPVKGYCFWALSDNWESENEHSSKFGLAAIDSDNNLQHITRPSYYLFSEVAKSGSITKGQRNKAWAELQTAIEQGNNHLICFGPDAQGGLENRVQRPFVKKDWRFGHYRPDGLQDPMSRTMRWICRAPHMISTRQERS*

Protein Length: 713

Fln nts: TTCCTCCTAAATGTGTCGAAGGTATTTGGTGTGGCAGGCTTCATTGCTGTTGCGGCCAACACCTTTGCCTTTTTTCGAGTTCACAGAAGGAAGTTGGCTCCCTTTGAAAATCCAATTGATGAGTCACAGGAGGTGCTCGCAAGCTTTCCAGTAAACAAAGATGTGGATGACGACGGCTTTTTCTTTGCCATTTCAACTGCTTCTGCTCTTCTAGAAGAAGATGCATGTCTTAAGTTTGCAGCCGAGTATGGTGATTATCCGAAGGAGATAAAACCAGAACCCAAAGATGGTAACCAATCGATTTTGTCTGAAGCACAAACTGATTTGCAAGTCAGAAGTAACCAGCAATTAGGCGAAGACAGAGAGAGTAAAGATGGATATGGTGGTCAAAACTTGGAAATTTCATCAACACTATCCGCAAAAAATCTATCAGAACACCATCAAATCAAGGAAGGTGGACGACAGAATAAGGATTTCTATGAAATGCCATTGGAGGAGTGGGAACTTTTAAATTATGAGCAAATGGCAGAAAGAGAACAGTGGTCTCGAGAACAAGTTTCCAGATTTCCTTCTGTAGCCGGCCATGGCAAATCAGAGGTAGAAAACATAGATTTGGATGATCCAAAAGACCTGCATAAATGTTCTGATCAGCATCTTGAACTTCATGGTAGTATTGCGGATAAGGGAGAGAAACTGTTGGATTCAACTCCTAAAGGGTTGATGAAGGGATCGGAAAATGTTACAGATGAACAGAAAGGAGTGGCTTCTCAGCTAAAACTTACTTGTCTAGAGGAAAAGCCTTGCTCCTGGTCTGATCCAGACCGAGAGCTGCATTTAGCTAAAGAAACGAATGCTACAGTTTTTCGCATGAGTATTGATTGGAACATAATAATGCCAGAAGAACCCCTTGATGGTTTTGAGAAGACTATCAATAGAACAGCACTAGATCGATACAGATGCAAAATTGAAAGAGTTCGTGCGTATGGATTGCGGGTGATGCTGACTCTTTTCCATCAATCTCTACCTAAATGGGCGTGTGCTTATGGTGGTTGGATGGAGGACAAAACTGTTGATTACTTTTTACAATATACAAGGCTAATGGTAGACAGTTTCTATGACCTGGTGGATTACTGGATAACATTCAATGAGCCACATTTATTTGCTTTGACCGAATATTGTTGTGGTGTCCCAGCCAACCTATTCCAAGTTGCTGCAACTGCTTTACATCAAAGAGTTTACAGAAGCACAATGGACAGAATCTTAACAGCTCATATAxxxxxxxxxTATGATATTATACATGGTACAGGAGAAAAGGCATCAAGAAAAGTAAGTGTTGGCATTGCACATCATACTTCTTTCATGCGACCATATGGCTTCTTTGATATTACTACAGTCGAGTTCAGCAATTGGATTGCATGCTTTGGCTATGTAGATCATGTCTTCAGCAAGCTGGACTTCTTAGGAATAAACTACCATGGGCAGGAATTCATTTCATCTGCTGGGTTGAAACTAGTTGAGAACGAGGAATACAGTGAATCAGGAAGATGCATATACCCAGATGGATTATATCGACTACTCCTTGACTTCCACAACCGCTATAAAATTTTTAATGTTCCTTTCATCATTACTGAAAATGGTGTTGCTGATGCCACTGATCTCCTTCGGAAGCCATATATTCTGGAACATCTCCTTGCTGTAAAAGCAGCCATGAATAAGGGTGTACCTGTTAAAGGTTACTGCTTTTGGGCTTTGTCAGACAACTGGGAATCTGAAAATGAGCATAGCTCCAAGTTTGGCCTTGCTGCTATAGACTCTGATAACAACCTGCAACATATTACTCGACCATCCTATTATCTCTTCTCTGAGGTTGCTAAATCTGGATCAATAACCAAGGGACAAAGGAATAAAGCTTGGGCTGAACTGCAAACTGCAATAGAGCAAGGGAACAACCACCTCATTTGCTTTGGGCCAGACGCTCAAGGTGGGTTGGAGAACCGAGTACAAAGGCCATTTGTTAAAAAGGATTGGAGGTTCGGTCACTATAGACCAGATGGATTACAAGATCCAATGAGCCGTACTATGAGATGGATATGCAGGGCACCGCACATGATATCCACAAGGCAAGAGAGATCGTAACTAAAG

Fln Alignment:

Contig633___LLNVSKXXXXXXXXXXXXNTFAFFRVHRRKLAPFENPIDESQEVLASFPVNKDVDDDGFFFAISTASALLEE---DACLKFAAEYGDYPKEIKPEPxxYDIIHGTGEKASRKVSVGIAHHTSFMRPYGFFDITTVEFSNWIACFGYVDHVFSKLDFLGINYHGQEFISSAGLKLVENEEYSESGRCIYPDGLYRLLLDFHNRYKIFNVPFIITENGVADATDLLRKPYILEHLLAVKAAMNKGVPVKGYCFWALSDNWESENEHSSKFGLAAIDSDNNLQHITRPSYYLFSEVAKSGSITKGQRNKAWAELQTAIEQGNNH-----LICFGPDAQGGLENRVQRPFVKKDWRFGHYRPDGLQDPMSRTMRWI
Q1M317______________LLNASKLVGVLATISVTANTIAFIRFRKKFLEPLDNPLDESEEVLATFDTDKNEDDGGFFFGLATAPAHVEDNLHDAWLEFAE---NDTADSNPQPxxFDLIHEFSKNSSLNARVGISHHVSFMRPYGLFDVPGVVLSNSMTLFPYIDSISEKLDFLGLNYYGQEVLSAPGLKLVYNDEYSESGRAVYPDGLYRMLLKFHERYKHLNLPFIITENGVSDATDLIRRPYILEHLLAVRAAMNKGVQVLGYLFWTTSDNWEWADGYGPKFGLVAVDRANNLARIPRPSYFLFSEVVKTGKVTRQQREIAWNELQIAAAEGKTRPFYRAVNKLGLMYSGGLDIPIQRPLIQRDWRFNHYQFDGLKDPLSCTIRFV

SNPs (tot: 11)

Position	A %	C %	G %	T %	Probability
1092	80			20	0.995493
1095	80		20		0.995493
1097			80	20	0.995493
1103	20		80		0.995493
1105		20		80	0.995493
1107	20		80		0.995493
1109		80		20	0.995493
1769	85		14		0.993533
1825	87		12		0.992554
1897		12	87		0.992554
2059	33			66	0.997452

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain