SustainPineDB

Assembly Name: biogeco2 v2.0

UniGene Name: biog2_v2.0_unigene19476

Length: 1624 nt

UniGene Fasta

Ace file of the UniGene biog2_v2.0_unigene19476

Annotations

Parent Assembly	Parent UniGene	Reads (related/total)
SustainPine v2.0	sp_v2.0_unigene24020	6/59
SustainPine v2.0	sp_v2.0_unigene25139	10/59
SustainPine v2.0	sp_v2.0_unigene60281	37/59
SustainPine v2.0	sp_v2.0_unigene60605	6/59

Source	Descriptions	Term	Type	Identity
AutoFact	Tubulin beta-4 chain n=11 Tax=Eukaryota RepID=TBB4_ORYSJ	-	-	94%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	97%
Blast2go	tubulin beta-4 chain	-	-	99%

Source	GOs	Term	Type	e value	Identity
Blast2go	cellular process	GO:0009987	Biological Process	0.0	99%
Blast2go	intracellular part	GO:0044424	Cellular Component	0.0	99%

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: D5ABQ9

Fln msg: Overlapping hits, possible frame ERROR between 139 and 136,

Fln protein: MREILHIQGGQCGNQIGAKFWEVVCDEHGIDPxxxYQGTSDLQLERVNVYYNEASCGRFVPRAVLMDLEPGTMDSVRTGPYGQVFRPDNFVFGQSGAGNNWAKGHYTEGAELIDSVLDVVRKEAENCDCLQGFQVCHSLGGGTGSGMGTLLISKIREEYPDRMMLTFSVFPSPKVSDTVVEPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLTTPSFGDLNHLISATMSGVTCCLRFPGQLNSDLRKLAVNLIPFPRLHFFMVGFAPLTSRGSQQYRALTVPELTQQMWDSKNMMCAADPRHGRYLTASAMFRGKMSTKEVDEQMMNVQNKNSSYFVEWIPNNVKSSACDIPPKGLSMSSTFIGNSTSIQEMFRRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYMQYQDASAEDDEYYDDEESAQES*

Protein Length: 447

Fln nts: TTCGATTTCCTGTGTCGCAAGGTTTTAGATCGCCAAG_-_ATGCGTGAAATCCTGCACATTCAGGGCGGCCAATGCGGCAACCAGATCGGTGCCAAGTTCTGGGAAGTTGTCTGTGATGAGCACGGAATCGATCCCxxxxxxxxxTACCAGGGCACTTCAGATCTACAGCTCGAAAGGGTTAATGTTTACTACAATGAGGCGAGCTGTGGGCGATTTGTTCCCCGTGCTGTGCTTATGGATCTGGAGCCTGGGACCATGGACAGTGTGAGGACCGGGCCTTATGGCCAGGTCTTTAGGCCTGATAACTTCGTTTTTGGCCAGTCCGGGGCTGGGAACAACTGGGCCAAGGGCCATTACACTGAGGGGGCTGAGCTCATTGATTCCGTTCTTGATGTCGTCAGGAAGGAGGCCGAGAACTGTGATTGCCTTCAAGGGTTTCAAGTGTGTCACTCTTTGGGGGGAGGCACTGGATCTGGAATGGGAACCCTCTTGATCTCCAAGATCCGAGAGGAATACCCTGATAGAATGATGCTCACTTTCTCTGTTTTCCCATCTCCCAAGGTGTCTGATACCGTGGTGGAGCCTTACAATGCTACACTTTCTGTGCATCAGCTGGTGGAGAATGCCGACGAGTGTATGGTGCTGGACAATGAGGCCCTCTACGACATCTGCTTCAGGACTCTCAAGCTCACGACACCTAGCTTTGGTGATTTGAACCATTTGATCTCTGCAACAATGAGTGGAGTCACCTGCTGCCTGAGGTTTCCCGGCCAGTTGAACTCAGATCTGAGGAAGCTGGCAGTGAATCTGATCCCTTTCCCCCGACTTCACTTTTTTATGGTTGGATTTGCCCCTTTGACCTCTCGTGGTTCACAGCAATACAGAGCCCTTACAGTTCCGGAGCTCACACAACAGATGTGGGATTCCAAGAACATGATGTGCGCTGCAGATCCACGTCATGGCAGGTATCTGACTGCATCTGCTATGTTCAGGGGCAAGATGAGTACCAAAGAGGTGGATGAGCAAATGATGAATGTTCAGAACAAGAATTCCTCCTACTTTGTGGAATGGATCCCTAATAATGTGAAATCCAGTGCATGTGATATTCCACCAAAAGGCCTGTCCATGTCGTCAACATTCATTGGTAACTCGACATCCATTCAGGAGATGTTCAGGAGAGTAAGTGAGCAGTTCACTGCTATGTTTAGGAGGAAGGCTTTCTTGCATTGGTACACTGGTGAGGGCATGGATGAGATGGAATTCACTGAAGCAGAGAGCAACATGAATGATTTGGTGTCTGAATACATGCAATATCAGGATGCTAGTGCAGAGGATGATGAATACTATGATGATGAAGAATCAGCTCAGGAAAGCTAG___GGCAGAAGCTTACGCAATGCCATCGACAACCGCTAGTTGTTTCAATGCCTAGTAGTTGTTTGTTTGTGGATTTCATTTGCATTTCTTGTTCTTTCATGGTCTGCTCAGCTCTCATCGAAGGTTTTGGCATTGAATGCATAAACTTTGTATACAACTCCTACATTATGTCCTTGGTTTTGTTGATCATATTGAAATATTGACGAGATTTTGAATTTGTTCATCTCATATAAACGTCATAATTGAATTTTTA

Fln Alignment:

biogeco2_mira_rep_c20080___MREILHIQGGQCGNQIGAKFWEVVCDEHGIDPMxRYQGTSDLQLERVNVYYNEASCGRFVPRAVLMDLEPGTMDSVRTGPYGQVFRPDNFVFGQSGAGNNWAKGHYTEGAELIDSVLDVVRKEAENCDCLQGFQVCHXXXXXXXXXXXXXXISKIREEYPDRMMLTFSVFPSPKVSDTVVEPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLTTPSFGDLNHLISATMSGVTCCLRFPGQLNSDLRKLAVNLIPFPRLHFFMVGFAPLTSRGSQQYRALTVPELTQQMWDSKNMMCAADPRHGRYLTASAMFRGKMSTKEVDEQMMNVQNKNSSYFVEWIPNNVKSSACDIPPKGLSMSSTFIGNSTSIQEMFRRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYMQ
D5ABQ9__________________MREILHIQGGQCGNQIGAKFWEVVCDEHGIDPTxRYQGTSDLQLERVNVYYNEASCGRFVPRAVLMDLEPGTMDSVRTGPYGQVFRPDNFVFGQSGAGNNWAKGHYTEGAELIDSVLDVVRKEAENCDCLQGFQVCHSLGGGTGSGMGTLLISKIREEYPDRMMLTFSVFPSPKVSDTVVEPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLTTPSFGDLNHLISATMSGVTCCLRFPGQLNSDLRKLAVNLIPFPRLHFFMVGFAPLTSRGSQQYRALTVPELTQQMWDSKNMMCAADPRHGRYLTASAMFRGKMSTKEVDEQMMNVQNKNSSYFVEWIPNNVKSSACDIPPKGLSMSSTFIGNSTSIQEMFRRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYMQ

SNPs (tot: 21)

Position	A %	C %	G %	T %	Probability
920			14	85	0.993533
1082		10		90	0.999898
1085		10		90	0.999898
1112		8		91	0.999766
1115	90		9		0.999842
1140	91	8			0.999807
1143	90		9		0.999842
1153			9	90	0.999842
1159	9		90		0.999842
1189	88		11		0.999952
1192		11		88	0.999952
1207		13		86	0.999973
1225		40		40	1.0
1267	66		33		0.997452
1293	60		40		1.0
1294			40	60	1.0
1330		80	20		0.995493
1386		75		25	0.996474
1415	83		16		0.994512
1481		16		83	0.994512
1530	85		14		0.993533

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain