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Fln status: Putative N-terminus

Fln database: coniferopsida.fasta

Fln subject: D5AA61

Fln msg: ERROR#3, very serious frame error, Warning!, your query overlaps and the subject is separated, Unexpected STOP codon at 3' end. Distance to subject end: 216 aas, atg_distance in limit (1-15): atg_distance = 16, No stop codon before M and low homology subject, your sequence is shorter than subject: 100 - 524

Fln protein: MGTLTNLLHMELSGCQSLESPQESW*FEQHMELIWCQSLERLPIFFSNLSELRxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxECTRICRSYPV*

Protein Length: 101

Fln nts: _-_ATGGGTACCTTGACAAACCTCCTACACATGGAGCTGTCTGGGTGCCAGAGTTTGGAGAGTCCGCAAGAGTCCTGGTAATTTGAACAACACATGGAGTTAATTTGGTGCCAGAGTTTGGAAAGGTTGCCAATTTTTTTTAGCAACTTGAGCGAACTCCGGGCxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxTAGAGTGTACAAGAATTTGCAGGAGCTACCCAGTGTAAAGACAATGGTATCTTTGGGATACTTGCAGGTGTCAGATCGTGTGAATTTGAAGAGCATACTAATTTATATAATTCTAAGCGGCCGGGGTGGGATGAGCGAGTACTAAACACGCTGTCCCAACCGAAAGGCTACTGAGATAAATTTGTAAATTTGGAGC

Fln Alignment:

biogeco2_mira_rep_c19561___MGTLTNLLHMELSGCQSLESPQESW*FE---QHMELIWCQSLERLPIFFSNLSEL------------RAPTH-------RTAAVVLVRGFGKATSSIW*LDNPPTYPPRVYKNLQELPSVKTMVSLGYLQVSDRVNLKSIxxxxxxxxxxxCEGLERLPAPFGNLTTLQHIHLxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxCEGLERLPAPFGNLTTLQHIHLECTRICRS
D5AA61__________________LGNLTNLQSMVLHECDHLERLPESLGNLTNLQSMELIYCKRLARLPKSLGNLTNLQSMQLLGLKSLKRLPKSLGNLMNLRSMQLLGLESLERLPKSLGNLTNLQSMELSFLESLERLPSIKTLLSLEELRVLDCVKLKSIxxxxxxxxxxxCGSLERLPESLGNLTNLQSMVLxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxCGNLERLPESLGNLMNLQSMKLKSERLPES