SustainPineDB

Assembly Name: biogeco2 v2.0

UniGene Name: biog2_v2.0_unigene1361

Length: 1619 nt

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene biog2_v2.0_unigene1361 (antisense)

Annotations

Parent Assembly	Parent UniGene	Reads (related/total)
SustainPine v2.0	sp_v2.0_unigene10090	45/47

Source	Descriptions	Term	Type	Identity
AutoFact	Cysteine synthase n=1 Tax=Picea sitchensis RepID=A9NRJ4_PICSI	-	-	88%
FL-Next	sp=Cysteine synthase; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	96%
Blast2go	cysteine synthase	-	-	92%

Source	ECs	Term	Type	e value	Identity
Blast2go	L-mimosine synthase.	EC:2.5.1.52	-	0.0	92%
Blast2go	Cysteine synthase.	EC:2.5.1.47	-	0.0	92%

Source	KEGGs	Term	Type	Identity
Blast2go	Cysteine and methionine metabolism	00270		92%
Blast2go	Sulfur metabolism	00920		92%
Blast2go	Metabolic pathways	01100		92%
Blast2go	Beta-pyrazolylalanine synthase.	EC:2.5.1.51	-	92%

Source	GOs	Term	Type	Identity
Blast2go	cysteine biosynthetic process from serine	GO:0006535	Biological Process	92%
Blast2go	L-mimosine synthase activity	GO:0050461	Molecular Function	92%
Blast2go	pyridoxal phosphate binding	GO:0030170	Molecular Function	92%
Blast2go	cysteine synthase activity	GO:0004124	Molecular Function	92%
Blast2go	beta-pyrazolylalanine synthase activity	GO:0047458	Molecular Function	92%
Blast2go	cytoplasm	GO:0005737	Cellular Component	92%

Source	InterPros	Term	Type	Identity
Sma3	Cysteine synthase/cystathionine beta-synthase P-phosphate-binding site	IPR001216	-	-
Sma3	Cysteine synthase K/M	IPR005856	-	-
Sma3	Cysteine synthase A	IPR005859	-	-
Sma3	Pyridoxal phosphate-dependent enzyme, beta subunit	IPR001926	-	-

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: A9NRJ4

Fln msg:

Fln protein: MAKDGINIAKDVTDLIGKTPMVYLNKVVDGCVAKIAAKLEIMEPCCSVKDRIGLSMISDAEEKGLIIPGRSVLVEPTSGNTGIGLAFIAAAKGYKLILTMPSSMSIERRVLLRAFGAELVLTDPARGMKGAVQKAEEIVEKTPNAYMLQQFENPSNPKIHYETTGPEIWQDTAGQIDAFVSGIGTGGTITGAGRYLKEKKPEVKIYGVEPIESAVISGGKPGPHKIQGIGAGFIPGNLDVNILDEVIQISSDEAVDMAKQLALKEGLLVGISSGAATAAAIRVAKRPENAGKLIVVILPSFGERYLSSVLFQSIKLEAENMVFEP*

Protein Length: 327

Fln nts: ATGGGGAGAACATACCAAACGCGTGCTACACTGAATTACGCAAATACATACGAAAAAATTATGCACCTTATCGGGTTCTGATTCCCTTGCGTTTGCCGAGAGTCACGAGGTCGCCGCATGCTTTTCTCGTCCCTACACCGACATCGGAAATTTTATATTTTATATACCCTGCGAAAATCAGTCCTGGGAACCATTAGGAGTGGAAGCATTTAGGAAGTTCATATCGACGATCTGAAGAGCTGCAAAAGAACACAGAA_-_ATGGCGAAGGACGGCATCAACATCGCTAAAGATGTGACCGATCTGATCGGGAAAACCCCAATGGTATATCTTAACAAAGTCGTAGATGGCTGCGTCGCCAAAATCGCAGCAAAGCTTGAGATTATGGAGCCTTGTTGCAGTGTCAAGGACAGGATTGGTTTAAGCATGATAAGCGATGCAGAGGAGAAAGGGCTTATTATTCCTGGAAGGAGTGTTTTGGTGGAGCCTACAAGTGGCAATACAGGAATAGGCCTTGCATTTATAGCCGCTGCAAAGGGATATAAACTTATTCTTACTATGCCTTCTTCTATGAGTATAGAGCGTAGGGTCTTACTGCGAGCTTTTGGAGCTGAATTGGTCCTTACAGATCCTGCCAGGGGTATGAAAGGTGCTGTACAGAAAGCAGAGGAAATAGTAGAAAAAACTCCTAATGCTTATATGCTTCAGCAGTTTGAGAACCCTTCTAATCCAAAGATTCACTATGAAACTACTGGACCTGAGATTTGGCAAGACACTGCTGGCCAAATAGATGCTTTTGTTTCTGGTATTGGAACAGGGGGTACGATTACTGGGGCAGGGCGTTATCTGAAGGAGAAGAAGCCAGAGGTCAAGATCTATGGTGTGGAGCCAATTGAGAGTGCTGTTATATCTGGAGGCAAGCCAGGCCCTCACAAAATTCAAGGAATTGGTGCTGGTTTTATACCTGGGAATCTGGATGTGAATATTCTTGATGAGGTCATACAGATTTCAAGTGATGAAGCTGTTGACATGGCCAAGCAGCTAGCATTAAAGGAAGGTTTGCTGGTGGGTATATCATCAGGTGCTGCGACTGCAGCAGCAATTAGAGTTGCAAAGAGGCCTGAAAATGCTGGGAAGTTGATTGTTGTGATACTCCCAAGCTTTGGAGAGCGGTATCTTTCTTCTGTACTTTTCCAATCAATCAAGTTGGAAGCTGAGAATATGGTTTTTGAACCATGA___AACTATGAAATTAGTAGTCATCCACACTGAAACTTTGTGCCCAAATTTGTTGAAGTGGTTCCATTACCGCTAGTTCTATCTTTTGATGGTTCTTATTGTTCCTGAAAATAATAGATCTGTTTAATGAGCAAAGGATGCGTTGAGTTTAACTTAATTTTCTTATATTTGGTGGGCATCCAATTTCATGTTGTTTTCTTAACCTTTTGTCAAGATATAGTTAGGATCCAATATGATGAATGTGCTACGTGTTTGAAAAATGCCTTGGAGACCATTTTACCGAGATGAACTTCATTCATGCTAACTCGAGCTGAAAATAATTGTCCAAAAACAGCTTTATCAATACTTTGCTATTTTGTTATATTTGGTGAGCACATCCAAATTCAT

Fln Alignment:

Contig1361___MAKDGINIAKDVTDLIGKTPMVYLNKVVDGCVAKIAAKLEIMEPCCSVKDRIGLSMISDAEEKGLIIPGRSVLVEPTSGNTGIGLAFIAAAKGYKLILTMPSSMSIERRVLLRAFGAELVLTDPARGMKGAVQKAEEIVEKTPNAYMLQQFENPSNPKIHYETTGPEIWQDTAGQIDAFVSXXXXXXXXXXXGRYLKEKKPEVKIYGVEPIESAVISGGKPGPHKIQGIGAGFIPGNLDVNILDEVIQISSDEAVDMAKQLALKEGLLXXXXXXXXXXXXXXXXXRPENAGKLIVVILPSFGERYLSSVLFQSIKLEAENMVFEP
A9NRJ4_________________MAKDGINIAKDVTDLIGKTPMVYLNKVADGCVAKIAAKLEIMEPCCSVKDRIGYSMISDAEEKGLIIPGRSILVEPTSGNTGIGLAFIAAAKGYKLILTMPSSMSIERRVLLRAFGAELILTDPARGMKGAVQKAEEIVEKTPNAYMLQQFENPSNPKIHYESTGPEIWQDTAGQIDAFVSGIGTGGTVTGVGRYLKEKKPEVQIYGVEPVESAVISGGKPGPHKIQGIGAGFIPGNLDVNILDEVIQISSDESVDMAKQLALKEGLLVGISSGAATAAAIRVAKRPENAGKLIVVILPSFGERYLSSVLFQSIKLEAENMVFEP

SNPs (tot: 25)

Position	A %	C %	G %	T %	Probability
638	80		20		0.995493
765		14		85	0.993533
1060	62	37			1.0
1132		12		87	0.992554
1171	12	87			0.992554
1174		12		87	0.992554
1175		12		87	0.992554
1176		12		87	0.992554
1178	12			87	0.992554
1179	12	87			0.992554
1180		87		12	0.992554
1342	44		55		1.0
1379	77	11	11		0.968132
1380		11	88		0.923564
1382		11		88	0.923564
1383	11	88			0.923564
1393		50		50	1.0
1398	50	50			1.0
1412	16			83	0.994512
1413			83	16	0.994512
1415		16		83	0.994512
1419	83	16			0.994512
1420		83		16	0.994512
1433		50	50		1.0
1438			50	50	1.0

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain