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Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: A9NT75

Fln msg: ERROR#3, very serious frame error, Overlapping hits, possible frame ERROR between 103 and 61,

Test code: 1

Test Code was used to find complete genes when there was not found a reliable orthologue. The best ORF (Open Reading Frame) is shown, and only ORFs > 200pb were analyzed. Your ORF will be more reliable if a stop codon was found before the start codon. A Test Code value > 0.95 means the ORF is probably coding. A Test Code value < 0.74 means the ORF is probably non-coding. Test Code values in between 0.74 and 0.95 mean it is uncertain whether the ORF is coding or not.

Fln protein: MATLKRSEISFxxxxxxxxxxxxxxxxGATTNFHRDLHFAAAAAQSVNQKSSASRAPQLRQSRSVGLIGAGYTRSRDHRVLNLLNGLNSADAQERTGKETGHKSQSRVIRWFKRTFLKSKS*

Protein Length: 123

Fln nts: ATTGTTGGATGAAGGGAATATTGGA_-_ATGGCGACTCTGAAGCGATCGGAGATTTCGTTCxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxGGTGCTACAACTAATTTCCACCGCGATTTACACTTCGCAGCCGCCGCCGCTCAGTCTGTTAATCAGAAATCCTCTGCCTCACGTGCTCCTCAGCTGAGGCAGAGCAGAAGCGTCGGATTAATTGGTGCCGGGTACACTCGATCGCGTGACCACCGTGTTCTGAACTTACTCAACGGTCTCAATTCTGCGGATGCACAGGAGCGCACTGGGAAGGAAACAGGTCACAAATCGCAATCTCGCGTAATCAGATGGTTCAAACGGACCTTCTTGAAATCCAAGAGTTGA___TTTTTATTTGATTTGTATACACAGTTCTTGTTTCTGTGTCTATCTGAGACTTGCATTAATTTCCTTCTAATAGAGGAAGTTAAGAAAATGTTTGGTTTGGACTTTGGAGTACATAGTTGGGGTTGAACCCTGTGTCCAGGGGTAG

Fln Alignment:

sp_v1.1_unigene83262___MATLKRSEISFRRQGxxxxxxxxxxx-ARVGGELDTG--------ATTNFHRDLHFAAAAAQSVNQKSSASRAPQLRQSRSVGLIGAGYTRSRDHRVLNLLNGLNSADAQERTGKETGHKSQSRVIRWFKRTFLKSKS
A9NT75_______________MATLKRSDISFRRQGxxxxxxxxxxxAAEVDGEAGGGPVFPPPPSGTTNSHRDLHYSATTA-----------SPQLRHSRSVGLIGAGYGRLRDHRGLN---GLNPANTQQRTHKETGHKSQSRVLRRFKRTFLKSKT