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Fln status: Internal

Fln database: tr_plants

Fln subject: A5ALS1

Fln msg: Unexpected STOP codon at 3' end. Distance to subject end: 432 aas, your sequence is shorter than subject: 79 - 1323

Test code: 0

Test Code was used to find complete genes when there was not found a reliable orthologue. The best ORF (Open Reading Frame) is shown, and only ORFs > 200pb were analyzed. Your ORF will be more reliable if a stop codon was found before the start codon. A Test Code value > 0.95 means the ORF is probably coding. A Test Code value < 0.74 means the ORF is probably non-coding. Test Code values in between 0.74 and 0.95 mean it is uncertain whether the ORF is coding or not.

Fln protein: ATRDAVGDPTDQRRTHSQFDRASSLLAQASVNYDPDTFAEASGHPD*EAAMNEEYHSLLANDTWDLVPLPKGRKLVRC*

Protein Length: 80

Fln nts: GCTACTCGCGATGCAGTAGGTGATCCTACAGATCAGCGACGTACACATTCTCAGTTTGATAGAGCCTCTTCTTTATTGGCTCAAGCTTCAGTGAATTATGATCCTGACACCTTTGCAGAAGCTTCAGGCCATCCAGATTGAGAAGCAGCTATGAATGAAGAATATCATTCATTATTGGCAAATGATACATGGGATCTTGTTCCTCTACCAAAGGGACGAAAACTTGTTAGATGCTAATGAGTGACAGCCAAGGTCACGACG

Fln Alignment:

sp_v1.1_unigene82853___SLLAQASVNYDPDTFAEASGHPD*EAAMNEEYHSLLANDTWDLVPLPKGRKLVRC
A5ALS1_______________SFVAQHSAVTEPRSYSEAAAHPEWQKAMRSELQALQANGTWSLTPLPAGKTPIGC