SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene81752

Length: 987 nt

Origin of reads: Biogeco_1 Uma

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v1.1_unigene81752 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	acid phosphatase, putative	-	-	42%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	76%
Blast2go	acid phosphatase	-	-	69%

Source	GOs	Term	Type	e value	Identity
Blast2go	vacuole	GO:0005773	Cellular Component	0.0	69%
Blast2go	plasma membrane	GO:0005886	Cellular Component	0.0	69%

Source	InterPros	Term	Type	e value	Identity
Blast2go	Acid phosphatase (Class B)	IPR005519	-	0.0	69%

Full-Lengther Next Prediction

Fln status: Putative Complete

Fln database: coniferopsida.fasta

Fln subject: D5ADB9

Fln msg: Overlapping hits, possible frame ERROR between 625 and 624, atg_distance in limit (1-15): atg_distance = 3, W2: There is no M at the beginning,

Fln protein: PTTPLSLVHTQHPNIHSPTPNNLKLFTDYKELSEQFTSHEETWEVKQENQLNFEAWRFGVETNTLRSWTVVPAECVEYVKSYMVGSGSQYLRDLDMVANESIAYVNSLKLSGDGKDAWVFDVDETLISTLPYFAAHQFGGEVIDDYDFNTRWTDLAEAPALPASHRLYAHLLELGFKIFLITGRHHYQRNGTERNLVRAGYHSWEALxxLREPEDYEKSAVLYKCEKRLKIEQDGFRIRGNSGDQWSDLNGYSVGDRTFKLPNPMYYVA*

Protein Length: 271

Fln nts: TCCCACGACTCCTCTGTCCCTCGTCCACACCCAACACCCTAATATCCACTCCCCAACACCCAATAACCTGAAACTCTTCACTGACTATAAAGAGCTGTCAGAGCAATTCACAAGCCATGAGGAGACGTGGGAGGTGAAGCAAGAGAACCAACTTAACTTTGAAGCCTGGAGGTTCGGCGTAGAAACAAACACTCTGCGCTCCTGGACAGTAGTGCCTGCAGAATGCGTGGAGTACGTGAAAAGCTACATGGTCGGAAGTGGAAGCCAATATTTGAGGGACTTAGACATGGTAGCCAACGAATCCATAGCTTATGTTAACTCTCTGAAACTCTCAGGCGATGGGAAGGACGCCTGGGTCTTCGACGTGGATGAGACCCTCATCTCAACTCTGCCCTATTTCGCAGCCCACCAGTTCGGCGGAGAGGTGATTGATGATTATGATTTTAATACGAGATGGACGGATCTGGCAGAGGCTCCGGCACTGCCTGCAAGTCATAGATTATACGCCCATCTTCTTGAACTGGGTTTCAAGATCTTCTTAATAACGGGGAGGCACCACTACCAGAGAAATGGCACAGAAAGGAATCTGGTCCGAGCTGGTTATCATTCATGGGAGGCGCTTxxxxxxCTCAGAGAGCCGGAAGATTATGAGAAGAGCGCTGTACTTTATAAGTGTGAGAAGAGATTGAAGATTGAGCAAGATGGGTTCAGGATCCGAGGGAACTCTGGTGATCAGTGGAGCGATTTGAATGGATACTCTGTTGGGGACAGAACATTCAAGTTGCCCAATCCCATGTATTACGTTGCCTGATATTATGGTTCTATGTGTTTCTATTGAATGTCCACTTTGTCGTCAACTTGATAAGTTTCTGCATCTGGTCTATATATGTACGTTGCAACTATAATGTTTGTTTCCAGATACGACTGTATTATGCAATTATAATGTTTTTTTCTCGATATAATATTGATGGTGTCAATAGTGTGAAAAA

Fln Alignment:

sp_v1.1_unigene81752___QHPNIHSPTPNNLKLFTDYKELSEQFTSHEETWEVKQ---ENQLNFEAWRFGVETNTLRSWTVVPAECVEYVKSYMVGSGSQYLRDLDMVANESIAYVNSLKLSGDGKDAWVFDVDETLISTLPYFAAHQFGGEVIDDYDFNTRWTDLAEAPALPASHRLYAHLLELGFKIFLITGRHHYQRNGTERNLVRAGYHSWEALxLREPEDYEKSAVLYKCEKRLKIEQDGFRIRGNSGDQWSDLNGYSVGDRTFKLPNPMYYVA
D5ADB9_______________QHPHIHSTTRKIPKVFNVY-----QSTVHEETSELKQSPEDYQVNCEAWRFGVETNTLRSWNVVPPECVEYVKNYMIGSGSQYVRDSNMVANESIAYVNSLQLSGDGKDAWVFDVDETLISTLPYFAAHQFGGEVIAEDDFNVKWLDRAVAPALPASHKLYARLLELGFKIFLLTGRRHCQRNVTERNLVRAGYHSWEALxLREPEDRAKSAVVYKSERRLKIEQNGFRIRGNSGDQWSDLTGYSVGDRTFKLPNPMYYVA

SNPs (tot: 13)

Position	A %	C %	G %	T %	Probability
7	66	33			0.997471
85	60			40	1.0
98		60		40	1.0
130	20	80			0.995493
146		40		60	1.0
241			40	60	1.0
270		60		40	1.0
294		71	28		1.0
370	33		66		0.997453
443		50		50	1.0
594		20	80		0.995493
595	80	20			0.995493
937	50		50		1.0

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain