SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene81326

Length: 1239 nt

Origin of reads: Uagpf Uma

UniGene Fasta

Ace file of the UniGene sp_v1.1_unigene81326

Annotations

Source	Descriptions	Term	Type	e value	Identity
AutoFact	acetylesterase, putative	-	-	2.0e-39	45%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	0.0	63%
Blast2go	lanatoside 15 -o-acetylesterase	-	-	0.0	66%

Full-Lengther Next Prediction

Fln status: C-terminus

Fln database: coniferopsida.fasta

Fln subject: B8LK87

Fln msg: Separated hits, possible frame ERROR between 396 and 426, your sequence is shorter than subject: 276 - 384

Fln protein: RRDLGCHSNFGGGANVVTLAVTARNESFIVPISLTVQVKEFEVFKQQVLATIKANGSLSNLPSAEAFNSGIYTIDIGGNDFSYGYMILKLNPTQVKTTILPKIAKSIAEAVQTLYNEGGRTFLVRDVAPQGxxxxxxxxxxxHSPNDFDKHGCSISYNDAVLHYNALLRAQLSSLRKQLKDATIIYVNTYDIIYDFFVNPSEYGFKATTKACCGVGGKYNYRYNIQCGASRNITGRTVKAVPCSDPDSCISWDGVHLTDEANRLPH*TNFEWKIL*

Protein Length: 277

Fln nts: CGTCGTGACCTTGGCTGTCACTCAAATTTCGGGGGTGGAGCCAACGTCGTGACCTTGGCTGTCACTGCTCGTAACGAGTCCTTCATCGTTCCCATCTCTCTCACTGTTCAGGTCAAAGAATTCGAGGTTTTCAAGCAGCAAGTTCTCGCCACCATCAAGGCGAACGGTTCTCTAAGCAATCTCCCAAGTGCTGAAGCGTTCAACAGCGGGATATACACAATAGATATCGGGGGAAACGACTTCAGCTACGGATACATGATTCTCAAACTCAACCCCACCCAGGTGAAAACTACCATTCTTCCAAAGATTGCAAAGTCTATTGCAGAAGCTGTGCAAACACTGTACAATGAGGGAGGGAGGACTTTTCTTGTGAGGGACGTGGCCCCTCAAGGGTGxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxTCATTCTCCCAACGACTTCGATAAGCACGGCTGCTCCATTTCCTACAACGACGCAGTTCTCCATTACAACGCACTCCTCAGAGCTCAGCTCTCCTCCCTTCGCAAGCAACTCAAAGACGCCACTATCATCTATGTCAATACATATGACATCATCTACGATTTCTTCGTCAACCCCTCCGAATATGGGTTTAAGGCCACCACTAAAGCATGCTGTGGAGTTGGAGGCAAGTACAATTACAGGTACAACATACAGTGTGGAGCCAGTAGAAACATCACTGGCCGCACTGTGAAGGCCGTGCCCTGCTCCGATCCAGACTCCTGCATCTCTTGGGATGGAGTCCACTTGACTGACGAGGCCAACAGACTCCCTCACTAAACAAATTTTGAGTGGAAAATACTTTGA___ACCGTCCTTCCCCCTCGCTAGCTTCTGTGATCTCCAACCCCTATGATTCATTCTTTCGCTAGCTTCTGTGATCTCCAACCCCTATGATTCATTCTTTTCTTTCTTGTCGGGGTTCCCTACTCGATTGGTAGGTTTGATGCAGCAGCCCTGCACTTGTTTCGGAATAAATAAGTTTCGGAATAAAGAAGAAGAAGCTTCTCCACATGCATTTTACGATTGAGATTATTCTAAGAACAGCTCATACATAGACATCACCCATTGACTTATGACTGTCTAAATAAGGGGAGTTTGACCATTGCAGTCTATGTACTCTAGGTTTATCTTTACCCATATCCATAGAAAATTATTATTGCCGTATATGTACTCTCTATTTATCTCTATCTATAGAAAATTATCATTGCAGTCTGTGTAC

Fln Alignment:

sp_v1.1_unigene81326___SNFGGGANVVTLAVTARN-ESFIVPISLTVQVKEFEVFKQQVLATIKANGSLSNLPSAEAFNSGIYTIDIGGNDFSYGYMILKLNPTQVKTTILPKIAKSIAEAVQTLYNEGGRTFLVRDVAPQGCxxxxxxxxxxHSPNDFDKHGCSISYNDAVLHYNALLRAQLSSLRKQLKDATIIYVNTYDIIYDFFVNPSEYGFKATTKACCGVGGKYNYRYNIQCGASRNITGRTVKAVPCSDPDSCISWDGVHLTDEANRL
B8LK87_______________SSFGHGANFAAAGATAVSVDSFIAPIDLTVQINQFKVFKQQVLNTIKKHGAQSYLPSADAFDKGIYILEIGGNDFSYGYKNLKQSPGQVKQSILPKVAKSVAAAVKELYNEGARTILVKDVGPQGCxxxxxxxxxxHSSNDFDSHGCSISYNDAVRYYNGLLKGQVGSLRGQLKGANVIYVNTYDILYDFIANPSRYGFKQTTRACCGVGGKYNYDYAVQCGISGTIAGHPVKAVSCAYPETYVNWDGVHWTDRANRI

SNPs (tot: 115)

Position	A %	C %	G %	T %	Probability
122	80		20		0.995493
126	20		80		0.995493
141	25		75		0.996473
144	75		25		0.996473
153		83		16	0.994516
154		83	16		0.994516
158	83			16	0.994516
161		77	22		0.999999
163	77			22	0.999999
164		10	90		0.903175
165			88	11	0.923564
166		90		10	0.903175
167	11		77	11	0.968132
168	88	11			0.923564
169	77	11		11	0.968132
170		88		11	0.923564
173	11		88		0.923564
175		11		88	0.923564
177	11	77	11		0.968132
182	90	10			0.90298
183			90	10	0.90298
185	90			10	0.90298
187		10		90	0.90298
211		88	11		0.923564
226	88		11		0.923564
231	88	11			0.923564
232			11	88	0.923564
251		88	11		0.923564
253	11		88		0.923564
256	88	11			0.923564
268	11			88	0.923564
292	12		87		0.992554
303		85		14	0.993533
305	85	14			0.993533
323	85		14		0.993533
325	14		85		0.993533
336	85		14		0.993533
349	85			14	0.993533
383	66			33	0.997452
389	33		66		0.997452
391			75	25	0.996481
393		66	33		0.997452
409	25		75		0.996486
413			50	50	1.0
414	20			80	0.995493
419	50		50		1.0
426			40	60	1.0
427	60		40		1.0
428		40		60	1.0
430	20		40	40	1.0
435		16		83	0.99452
463		80		20	0.995493
489		75	25		0.996473
492		25		75	0.996473
503	87		12		0.992555
504	87			12	0.992555
506	14		85		0.993533
508	87			12	0.992555
509		87	12		0.992555
510	12			87	0.992555
514		85		14	0.993533
515	85		14		0.993533
517	83	16			0.994512
518			83	16	0.994512
519		83	16		0.994512
524			83	16	0.994512
541	55		33	11	1.0
542	90	10			0.903175
544		90		10	0.903175
545	90		10		0.903175
550	88			11	0.923564
551	88			11	0.923564
552	88		11		0.923564
554	88	11			0.923564
555		88		11	0.923564
557		88		11	0.923564
561		33		55	1.0
600		90		10	0.903003
605		37		62	1.0
615		57		42	1.0
616	42		57		1.0
631	42		57		1.0
641	57	42			1.0
662	42		57		1.0
674	50			50	1.0
676	57			42	1.0
677	50		50		1.0
678			50	50	1.0
688		60		40	1.0
689	60		40		1.0
697			60	40	1.0
703	60		40		1.0
714		25		75	0.996473
729			50	50	1.0
736		60	40		1.0
745	25		75		0.996473
747		50		50	1.0
758	50		50		1.0
760	60		40		1.0
763	50			50	1.0
764	50	50			1.0
782			40	60	1.0
802	50	50			1.0
832	83			16	0.994514
881		66	33		0.997452
884	75		25		0.996481
889		25		75	0.996481
891	66			33	0.997452
894			33	66	0.997452
897			33	66	0.997452
900	33		66		0.997452
945			50	50	1.0
985		50		50	1.0
1139	40	60			1.0
1179		50		50	1.0

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain