SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene78338

Length: 1537 nt

Origin of reads: Biogeco_1 Biogeco_2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v1.1_unigene78338 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	CBL-interacting protein kinase 16 n=2 Tax=Vitis vinifera RepID=C4P7W5_VITVI	-	-	66%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	78%
Blast2go	serine-threonine kinase	-	-	77%

Source	InterPros	Term	Type	Identity
Blast2go	Protein kinase, catalytic domain	IPR000719	-	77%
Blast2go	Serine/threonine- / dual-specificity protein kinase, catalytic domain	IPR002290	-	77%
Blast2go	NAF domain	IPR004041	-	77%
Blast2go	Serine/threonine-protein kinase, active site	IPR008271	-	77%
Blast2go	Protein kinase-like domain	IPR011009	-	77%
Blast2go	Serine/threonine-protein kinase-like domain	IPR017442	-	77%
Blast2go	NAF/FISL domain	IPR018451	-	77%
Blast2go	Tyrosine-protein kinase, catalytic domain	IPR020635	-	77%

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: A9NWN4

Fln msg: Overlapping hits, possible frame ERROR between 316 and 313,

Fln protein: MKNAPNILMGRFELGRLLGQGAFAKVYHARNLQTGESVAIKAMDRxxxLKGGMIEHIKREISIMRLVRHPNIVRLYEVMASKTKIYFVMEYVKGGELFNRVLQKGKLKEEEARKYFQQLISAVDFCHSRGVYHRDLKPENLLLDENRDLKVTDFGLSALPAQFRQDGLLHTACGTPAYIAPEVLNKKGYDGDKADIWSCGVVLFVLMAGCLPFQGANLMVMYKKIYKGDFKCPPWFSSDARRVVVRLMDPNPATRITIPQLMEVSWFRRGFKRAEAMNDLNVNLLDDVESAFFTAKPACLNAFDIISLSPGFNLSGLFEDKVATQESSTFTSTQPVSRIMSRLEEIAKALRFKVTKSKDCKVKLQDSKKGRKGHLCIDAEIFEVTPSLFVVELGKTGGDTPEYSKFCTQVLRPGLKDIVWEWAGDNQGVG*

Protein Length: 432

Fln nts: ATGGGGAGTCGCGGAGAAACAGACAGGCCAACGTACTGAGAGGCAGGTGGCACCGTTGCATCACAAGGGCGGCATAAAGATATGCATTTCTATGTTTTAAAGCCATGGAAATTGCCTCTGTGTTTAGATTAAAAGGAACAGGGTTGAGCAGATCGATTTGAGTTTGTAGGCACAG_-_ATGAAGAATGCACCCAATATTTTGATGGGGAGGTTCGAATTAGGGCGCCTACTGGGGCAAGGGGCGTTCGCCAAGGTCTACCACGCGAGAAACCTCCAAACTGGCGAGAGCGTCGCCATTAAAGCCATGGACAGAxxxxxxxxxCTAAAGGGCGGCATGATCGAGCACATTAAAAGAGAAATCTCGATTATGAGACTGGTCCGGCACCCCAACATCGTTCGGCTCTACGAAGTCATGGCGAGCAAGACCAAGATTTACTTCGTCATGGAGTACGTGAAGGGCGGCGAGCTCTTCAATCGGGTTCTCCAGAAGGGCAAACTGAAGGAAGAGGAGGCTAGGAAGTATTTTCAGCAGCTGATAAGTGCGGTGGACTTCTGCCACAGCCGAGGCGTGTACCACCGGGACCTAAAGCCAGAGAATTTGTTATTAGACGAAAACAGGGACTTGAAGGTCACAGACTTCGGCCTCAGCGCGCTGCCGGCGCAGTTCAGGCAGGATGGTCTTCTCCACACGGCGTGCGGCACGCCGGCCTACATCGCACCGGAGGTCCTTAACAAGAAGGGCTACGACGGGGACAAGGCCGATATCTGGTCCTGCGGTGTCGTACTGTTCGTCCTCATGGCGGGCTGTCTTCCGTTCCAAGGCGCGAATCTGATGGTGATGTATAAGAAGATCTACAAGGGGGATTTCAAGTGCCCGCCCTGGTTTTCCTCCGATGCCCGGCGGGTCGTCGTTCGCTTGATGGACCCGAATCCCGCAACGAGGATCACCATTCCGCAGTTAATGGAGGTCTCCTGGTTCCGCCGGGGTTTCAAGCGAGCGGAAGCCATGAACGATTTAAATGTCAACCTTCTCGACGATGTCGAGTCGGCGTTCTTCACCGCCAAACCGGCCTGTCTTAACGCCTTCGACATCATTTCCCTCTCGCCGGGGTTCAATCTCTCGGGGCTGTTCGAGGACAAGGTCGCAACGCAGGAATCGTCGACGTTCACATCTACTCAGCCGGTGTCGAGAATAATGTCTAGGCTGGAGGAGATTGCGAAGGCGCTGAGATTCAAGGTTACGAAATCTAAGGACTGCAAGGTGAAGTTGCAGGACTCCAAGAAGGGGCGCAAGGGCCATTTGTGTATTGATGCCGAGATTTTTGAAGTAACGCCGTCGCTCTTCGTTGTAGAACTTGGCAAGACCGGGGGTGATACGCCCGAGTACAGTAAGTTCTGTACGCAGGTCCTCAGGCCAGGTCTCAAGGACATCGTATGGGAATGGGCCGGTGACAATCAAGGGGTGGGATAA___GGGGCACCCAGTGGAACGAGAAGTAGATATTCAGTGTGTATACATCACCGCGCTTGTTGATACTTTACTA

Fln Alignment:

sp_v1.1_unigene78338___MKNAPNILMGRFELGRLLGQGAFAKVYHARNLQTGESVAIKAMDRExVLKGGMIEHIKREISIMRLVRHPNIVRLYEVMASKTKIYFVMEYVKGGELFNRVLQKGKLKEEEARKYFQQLISAVDFCHSRGVYHRDLKPENLLLDENRDLKVTDFGLSALPAQFRQDGLLHTACGTPAYIAPEVLNKKGYDGDKADIWSCGVVLFVLMAGCLPFQGANLMVMYKKIYKGDFKCPPWFSSDARRVVVRLMDPNPATRITIPQLMEVSWFRRGFKRAEAMN--DLNVNLLDDVESAFFTAKPACLNAFDIISLSPGFNLSGLFEDKVATQESSTFTSTQPVSRIMSRLEEIAKALRFKVTKSKDCKVKLQDSKKGRKGHLCIDAEIFEVTPSLFVVELGKTGGDTPEYSKFCTQVLRPGLKDIVWEWAGDNQ
A9NWN4_______________MNIAPNILMGRYEVGRLLGQGSFAKVYLARNLETGQSVAIKAMNIExILKGGMIEQIKREISIMRLVQHPNIVLLYEVMASKTKIYFVMEYVKGGELFNRVVEKGRLKEEEARKYFQQLISAVDFCHSRGVYHRDLKPENLLLEENGDLKVADFGLSALPAQFKQDGLLHTTCGTPAYLAPEVVSNKGYNGDKADIWSCGVILFVLMAGYLPFQCANLMVMYKKIDKGDFKCPPWFSCDARRLVLRLMDPNPRTRMTVPQLMEVPWFRRGFKRPQAKNEVDVDVGVLDEVESAFFTAKPACLNAFDIISLSPGFSLSGLFKDKVVKQESSRFMSIQPASSIMSKLEEIARVLRFSVKKSKNCKVELQDSKKGRKGHLCIDAEIFEVTPSLFLVELGKTGGDTLEYSKFCSQALKPGLKDIVWAWDGDNQ

SNPs (tot: 18)

Position	A %	C %	G %	T %	Probability
796	20	80			0.995505
879		20		80	0.995493
904		25	75		0.996473
951		20		80	0.995493
1073			33	66	0.997452
1078		33	66		0.997452
1085	33	66			0.997452
1086	33	66			0.997452
1088		66	33		0.997452
1089		66		33	0.997452
1091	33			66	0.997453
1092		33		66	0.997453
1093		66	33		0.997452
1251		25		75	0.996474
1316	75		25		0.996473
1387	66	33			0.997453
1388		33		66	0.997453
1390		33	66		0.997453

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain