SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene77518

Length: 804 nt

Origin of reads: Biogeco_1 Uagpf Uma

UniGene Fasta

Ace file of the UniGene sp_v1.1_unigene77518

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	gpm370; LOC100274407; K13421 uridine monophosphate synthetase [EC:2.4.2.10 4.1.1.23]	-	-	56%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	85%
Blast2go	ump synthase	-	-	72%

Source	ECs	Term	Type	e value	Identity
Blast2go	Orotidine-5'-phosphate decarboxylase.	EC:4.1.1.23	-	0.0	72%

Source	KEGGs	Term	Type	Identity
Blast2go	Pyrimidine metabolism	00240		72%
Blast2go	Metabolic pathways	01100		72%
Blast2go		EC:2.4.2.1-	-	72%

Source	ECs	Term	Type	e value	Identity
AutoFact		EC:2.4.2.1-	-	0.0	56%
AutoFact	Orotidine-5'-phosphate decarboxylase.	EC:4.1.1.23	-	0.0	56%

Source	KEGGs	Term	Type	e value	Identity
AutoFact	Pyrimidine metabolism	00240		0.0	56%
AutoFact	Metabolic pathways	01100		0.0	56%

Source	GOs	Term	Type	Identity
Blast2go	pyrimidine ribonucleotide biosynthetic process	GO:0009220	Biological Process	72%
Blast2go	response to cadmium ion	GO:0046686	Biological Process	72%
Blast2go	cellular response to phosphate starvation	GO:0016036	Biological Process	72%
Blast2go	orotidine-5'-phosphate decarboxylase activity	GO:0004590	Molecular Function	72%
Blast2go	orotate phosphoribosyltransferase activity	GO:0004588	Molecular Function	72%

Source	InterPros	Term	Type	Identity
Blast2go	Orotidine 5'-phosphate decarboxylase domain	IPR001754	-	72%
Blast2go	Ribulose-phosphate binding barrel	IPR011060	-	72%
Blast2go	Aldolase-type TIM barrel	IPR013785	-	72%

Full-Lengther Next Prediction

Fln status: Internal

Fln database: coniferopsida.fasta

Fln subject: D5A9B8

Fln msg: Separated hits, possible frame ERROR between 589 and 592, Unexpected STOP codon at 3' end. Distance to subject end: 121 aas, your sequence is shorter than subject: 229 - 483

Fln protein: VKDYGTRKSIEGVFSPGHTCLVIEDLVTSGASVMETVEPLKSVVTLAVTHVVLIDREQGGSDNLVSNGIQLHSALKLTQMVDVLVRHDKLSGEIADSVRTFIAENQTKVPAPSPSPAPVNGVRKRSSYGERAALAQNPTGRKLFELMERKKTNLSLAADVSTAQELLSIADAVGPEICLLKTHVDILPDFTPDFGxxLRAIADKHDFLIFEDRKFADIGNTVTMQYEG*

Protein Length: 230

Fln nts: GGTCAAGGATTATGGCACCaGAAAATCCATTGAAGGAGTCTTCAGCCCAGGGCATACATGCCTGGTTATTGAAGATCTGGTGACAAGCGGGGCCTCTGTAATGGAGACAGTTGAGCCTCTTAAATCCGTCGTGACCTTGGCTGTCACTCATGTTGTTCTGATTGACAGAGAGCAGGGTGGGTCAGATAACTTGGTCAGCAATGGCATTCAATTGCACTCGGCCCTTAAGCTCACACAAATGGTGGATGTGCTCGTGAGGCATGACAAGCTATCTGGAGAGATCGCGGATTCTGTCAGGACGTTCATCGCAGAGAACCAGACGAAGGTGCCTGCCCCTTCCCCTTCTCCTGCCCCTGTTAACGGTGTTAGGAAGAGGAGCTCTTATGGTGAGAGGGCCGCTCTTGCTCAGAACCCAACAGGCAGGAAGCTGTTTGAGCTCATGGAGAGGAAGAAGACTAATCTATCTCTAGCAGCCGATGTTTCCACAGCCCAAGAGCTTCTCAGCATTGCGGATGCGGTTGGGCCTGAGATATGCCTTCTGAAAACACATGTTGACATACTACCTGACTTCACACCTGACTTTGGTGCxxxTCTTCGTGCGATTGCAGACAAGCACGATTTCTTAATTTTTGAAGATAGGAAGTTTGCTGACATTGGAAATACAGTGACCATGCAATATGAAGGGTAAGTAATAGTTGTGTTTGATACACATTATAATTAACATGCTTTTTTCATGGGTCATACCTAAGGTTGGCTAGGATTTCTTGGAGACTTGGCTGGAGTGGACATTCTCTGTTACACTGGG

Fln Alignment:

sp_v1.1_unigene77518___VKDYGTRKSIEGVFSPGHTCLVIEDLVTSGASVMETVEPLKSVVTLAVTHVVLIDREQGGSDNLVSNGIQLHSALKLTQMVDVLVRHDKLSGEIADSVRTFIAENQTK----XXXXXXXXXXXNGVRKRSSYGERAALAQNPTGRKLFELMERKKTNLSLAADVSTAQELLSIADAVGPEICLLKTHVDILPDFTPDFGAxLRAIADKHDFLIFEDRKFADIGNTVTMQYEG*VIVVFD
D5A9B8_______________VKDYGTKKSIEGVFSPGQTCLVIEDLVTSGASVLETVGPLKSVGLNVSDTVVLIDREQGGSDNLANNGIALHSALKLTQMVDVLVRHSKLSAEIADSVRKFIAENQTKVPVPSPSPSLAPAPVDGVRKRSSYGERAALAQNPTGRKLFELMERKKTNLSLAADVSTAQQLVCIADAVGPEICVLKTHVDILPDFTPDFGAxLRAIADKHDFLIFEDRKFADIGNTVTMQYEGGIFKIVE

SNPs (tot: 10)

Position	A %	C %	G %	T %	Probability
336		83	16		0.994513
337		83		16	0.994513
339			16	83	0.994512
341		60	20		0.99255
342	20	80			0.995495
351		80	20		0.995493
353		20	80		0.995493
354	20	80			0.995493
356		80		20	0.995493
358	20			80	0.995493

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain