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Fln status: Internal

Fln database: coniferopsida.fasta

Fln subject: B8LL66

Fln msg: Separated hits, possible frame ERROR between 179 and 296, and possible frame ERROR between 397 and 505, Unexpected stop codon in the beginning of your sequence, Distance to subject end: 16 aas, your sequence is shorter than subject: 209 - 459

Test code: 0

Test Code was used to find complete genes when there was not found a reliable orthologue. The best ORF (Open Reading Frame) is shown, and only ORFs > 200pb were analyzed. Your ORF will be more reliable if a stop codon was found before the start codon. A Test Code value > 0.95 means the ORF is probably coding. A Test Code value < 0.74 means the ORF is probably non-coding. Test Code values in between 0.74 and 0.95 mean it is uncertain whether the ORF is coding or not.

Fln protein: LVSLGFEWLYRSRQE*YLQ*LPSNFQGETLFQTHENLEHLAMMERVLGPLPHHMLKKSxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxRYVKYFRQGTRLNWPEGASSRESIRAVRKLPKLxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxLQDLVMKHADNSAGLFIDLLQGLLNYEPSERLKASEALRHP

Protein Length: 210

Fln nts: GACTAGTTTCTCTTGGATTTGAATGGTTATATAGATCCCGGCAGGAATGATATTTACAGTAACTGCCTTCAAATTTTCAGGGTGAGACTTTGTTTCAAACTCATGAAAACTTGGAACATCTTGCTATGATGGAAAGGGTATTAGGGCCTTTGCCACATCATATGTTGAAAAAATCAGAxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxAAGATATGTGAAATATTTTAGACAAGGAACACGATTGAATTGGCCTGAAGGAGCATCCTCTAGAGAGAGCATCAGAGCAGTGAGAAAGTTACCAAAACTTAxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxGTTTGCAGGATTTGGTAATGAAACATGCAGACAACTCAGCAGGTCTATTTATAGATTTGTTGCAGGGGCTTCTCAATTATGAACCCTCAGAACGCTTGAAAGCTTCAGAGGCTTTGAGACATCCAT

Fln Alignment:

sp_v1.1_unigene75810___GETLFQTHENLEHLAMMERVLGPLPHHMLKKSExxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxRYVKYFRQGTRLNWPEGASSRESIRAVRKLPKLRxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxLQDLVMKHADNSAGLFIDLLQGLLNYEPSERLKASEALRHP
B8LL66_______________GETLFQTHENMEHLAMMERVLGPFPQHLIRKADxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxRSAKYFRHGTRLNFPEGASSRESIRAVKKLPRLRxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxLRNLVMERADHSAGALIDLLQGLLKYEPSERLEAQEALRHP