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Fln status: Putative N-terminus

Fln database: coniferopsida.fasta

Fln subject: A9NTD9

Fln msg: Warning!, your query overlaps and the subject is separated, and overlapping frame ERROR between 307 and 303, Distance to subject end: 114 aas, atg_distance in limit (1-15): atg_distance = 8, W2: There is no M at the beginning, your sequence is shorter than subject: 131 - 301

Test code: 0

Test Code was used to find complete genes when there was not found a reliable orthologue. The best ORF (Open Reading Frame) is shown, and only ORFs > 200pb were analyzed. Your ORF will be more reliable if a stop codon was found before the start codon. A Test Code value > 0.95 means the ORF is probably coding. A Test Code value < 0.74 means the ORF is probably non-coding. Test Code values in between 0.74 and 0.95 mean it is uncertain whether the ORF is coding or not.

Fln protein: RRDLGCHSIFGFQVLSKRLLPTGTLETGSTNKRTSLSFSTASANKPRGGLPRFYSEVLPTGKDKIVRIEGDEFWHMTKALRLGVQDxxTLKGGRADWLIExxxxGASSVTPLLTERSTLISENRVDRWQRV

Protein Length: 132

Fln nts: CGTCGTGACCTTGGCTGTCACTCAATTTTTGGATTTCAGGTGCTTAGTAAAAGGTTGCTACCCACCGGAACATTGGAGACTGGCTCTACTAACAAGCGCACTTCATTGAGTTTTTCCACTGCTTCTGCAAACAAGCCACGGGGAGGTCTTCCTCGCTTCTATTCCGAGGTTCTCCCTACTGGAAAGGACAAAATTGTGCGCATAGAGGGTGATGAGTTTTGGCACATGACAAAAGCATTGCGCTTAGGTGTTCAAGATxxxxxxACTTTGAAAGGAGGTCGAGCTGATTGGCTTATAGAGAxxxxxxxxxTGGGGGCAAGCAGCGTTACACCGTTGCTGACAGAGAGATCGACTTTGATCAGTGAAAATCGGGTTGACAGGTGGCAACGTGTAAT

Fln Alignment:

sp_v1.1_unigene70905___SIFGFQVLSKRLLPTGTLETGSTNKRTSLSFSTASANKPRGGLPRFYSEVLPTGKDKIVRIEGDEFWHMTKALRLGVQDRxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxGTLKGGRADWLIEK*TELGASSVTPLLTERSTLISENRVDRWQRV
A9NTD9_______________NIFGLQVLSKKLLPTGTLEIGSTYKPTSSSFSTASANKPRGGLPRFYSEVLPAGKDKIVRIEGDEFWHMTKALRLGVQDRxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxGTLKGGRADWLIEKCTELGASSVTPLLTERSTLISGNRVERWQRV