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Fln status: Internal

Fln database: coniferopsida.fasta

Fln subject: A9NWA3

Fln msg: ERROR#3, very serious frame error, Overlapping hits, possible frame ERROR between 198 and 198, Distance to subject end: 156 aas, your sequence is shorter than subject: 133 - 426

Test code: 1

Test Code was used to find complete genes when there was not found a reliable orthologue. The best ORF (Open Reading Frame) is shown, and only ORFs > 200pb were analyzed. Your ORF will be more reliable if a stop codon was found before the start codon. A Test Code value > 0.95 means the ORF is probably coding. A Test Code value < 0.74 means the ORF is probably non-coding. Test Code values in between 0.74 and 0.95 mean it is uncertain whether the ORF is coding or not.

Fln protein: ENVLLDGNGDVKVSDFGLSALPQQFGQDGLLHTACGTPAYVAPEVITKKGYDGAKADIWSCGVILxxLMAGYLPFQEANMMAMYRKIYKGDFKCPPWFSSEVRTLILRLLDPKPQTRITIAQLMQVPWFQRSL

Protein Length: 134

Fln nts: GAGAACGTTTTGTTGGACGGCAACGGGGATGTGAAGGTCTCAGACTTCGGGCTCAGCGCGCTGCCTCAACAATTCGGCCAGGACGGCCTTCTCCATACGGCATGCGGCACACCGGCCTACGTCGCACCGGAGGTCATTACAAAGAAGGGCTACGATGGTGCCAAGGCCGACATCTGGTCGTGCGGGGTCATACTGxxxxxxCTCATGGCGGGCTACCTGCCGTTCCAAGAGGCGAATATGATGGCGATGTATAGGAAGATTTACAAGGGAGACTTCAAGTGTCCGCCATGGTTTTCCTCCGAAGTCCGAACGCTCATCCTCCGGTTGCTGGATCCGAAGCCCCAGACAAGGATCACCATTGCGCAGCTGATGCAGGTTCCATGGTTCCAGCGGAGCTTGA

Fln Alignment:

sp_v1.1_unigene68916___ENVLLDGNGDVKVSDFGLSALPQQFGQDGLLHTACGTPAYVAPEVITKKGYDGAKADIWSCGVILWVLMAGYLPFQEANMMAMYRKIYKGDFKCPPWFSSEVRTLILRLLDPKPQTRITIAQLMQVPWFQR
A9NWA3_______________ENLLLDENGDMKVSDFGLSALPEQFRQDGLLHTACGTPAYVSPEVITKKGYDGAKADIWSCGVILFVLMAGYLPFLDANLMVMYKKIYKGDFKCPPWFSSEVRRLVVRLLDPNPRTRITVPQLMEVPWFRR