SustainPineDB

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Assembly info
Unigenes
SNPs
SSRs
Descriptions
GOs
ECs
KEGGs
InterPros
miRNAs

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene66435

Length: 520 nt

Origin of reads: Biogeco_1 Biogeco_2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>sp_v1.1_unigene66435
TTGGGTGATAAGTTAAGATAATTAGGCCATAGGCCTTCTTTTGAATCAGTTCGACCCTCTAAATTTTTAATGTCCATTTGACATCTGTTCACAGATTTTAAGCCATCAAAAAACTCTCCACTTGACATCTTTGCGCACCTTTTAAGCCGAGAGGTTCCGGGAGAGTTCGTGGAGTTTATGGCACTGACCATAAAACTTATGGTGCACTATTGGACTGCATCTATATCGTGTGACCAGTTCGAGGTGGTTTTTTCAGGCGGTTGGAGCTTTGGATTGCGGACCGGGTGGTGCTGCCGGTGGAGAACTCACTTGGCGGAAACATCCACCACAACTATGATATGCTCCTCTGCCACAAGCTTCAATTTGTGGACGAGGTGCAGTTGCCCGTGCACCACTGTCTAATGGCGCTCCCGAGCACGAGGAAGGAAGATGTGCGGCGCGACCAACCACCCGCAGGCCCTTGCCCAGTGCGAGCACACGATTTTGAAGCTCATCGGCCAGGAAAAACAACATTCCCCAT

Ace file of the UniGene sp_v1.1_unigene66435 (antisense)

If you push the Download ace button, you will download an ace file of this UniGene.

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Annotations

Source	Descriptions	Term	Type	e value	Identity
AutoFact	Arogenate dehydratase/prephenate dehydratase 6, chloroplastic n=1 Tax=Arabidopsis thaliana RepID=AROD6_ARATH	-	-	8.0e-23	71%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	0.0	74%
Blast2go	arogenate prephenate dehydratase	-	-	1.08354e-25	81%

Source	ECs	Term	Type	e value	Identity
Blast2go	Arogenate dehydratase.	EC:4.2.1.91	-	1.08354e-25	81%

Source	KEGGs	Term	e value	Identity
Blast2go	Phenylalanine, tyrosine and tryptophan biosynthesis	00400	1.08354e-25	81%
Blast2go	Biosynthesis of phenylpropanoids	01061	1.08354e-25	81%
Blast2go	Biosynthesis of alkaloids derived from shikimate pathway	01063	1.08354e-25	81%
Blast2go	Biosynthesis of plant hormones	01070	1.08354e-25	81%
Blast2go	Metabolic pathways	01100	1.08354e-25	81%
Blast2go	Biosynthesis of secondary metabolites	01110	1.08354e-25	81%

Source	GOs	Term	Type	e value	Identity
Blast2go	tyrosine biosynthetic process	GO:0006571	Biological Process	1.08354e-25	81%
Blast2go	L-phenylalanine biosynthetic process	GO:0009094	Biological Process	1.08354e-25	81%
Blast2go	response to low fluence blue light stimulus by blue low-fluence system	GO:0010244	Biological Process	1.08354e-25	81%
Blast2go	arogenate dehydratase activity	GO:0047769	Molecular Function	1.08354e-25	81%
Blast2go	chloroplast	GO:0009507	Cellular Component	1.08354e-25	81%
Blast2go	cytosol	GO:0005829	Cellular Component	1.08354e-25	81%

Full-Lengther Next Prediction

Fln status: Internal

Fln database: coniferopsida.fasta

Fln subject: C0PQ13

Fln msg: Separated hits, possible frame ERROR between 440 and 445, Unexpected stop codon in the beginning of your sequence, Distance to subject end: 173 aas, your sequence is shorter than subject: 173 - 443

Fln protein: G**VKIIRP*AFF*ISSTL*IFNVHLTSVHRF*AIKKLST*HLCAPFKPRGSGRVRGVYGTDHKTYGALLDCIYIV*PVRGGFFRRLELWIADRVVLPVENSLGGNIHHNYDMLLCHKLQFVDEVQLPVHHCLMALPSTRKEDVRxxxNHPQALAQCEHTILKLIGQEKQHSP

Protein Length: 174

Fln nts: TTGGGTGATAAGTTAAGATAATTAGGCCATAGGCCTTCTTTTGAATCAGTTCGACCCTCTAAATTTTTAATGTCCATTTGACATCTGTTCACAGATTTTAAGCCATCAAAAAACTCTCCACTTGACATCTTTGCGCACCTTTTAAGCCGAGAGGTTCCGGGAGAGTTCGTGGAGTTTATGGCACTGACCATAAAACTTATGGTGCACTATTGGACTGCATCTATATCGTGTGACCAGTTCGAGGTGGTTTTTTCAGGCGGTTGGAGCTTTGGATTGCGGACCGGGTGGTGCTGCCGGTGGAGAACTCACTTGGCGGAAACATCCACCACAACTATGATATGCTCCTCTGCCACAAGCTTCAATTTGTGGACGAGGTGCAGTTGCCCGTGCACCACTGTCTAATGGCGCTCCCGAGCACGAGGAAGGAAGATGTGCGGCGxxxxxxCAACCACCCGCAGGCCCTTGCCCAGTGCGAGCACACGATTTTGAAGCTCATCGGCCAGGAAAAACAACATTCCCCAT

Fln Alignment:

sp_v1.1_unigene66435___FRRLELWIADRVVLPVENSLGGNIHHNYDMLLCHKLQFVDEVQLPVHHCLMALPSTRKEDVRRxxNHPQALAQCEHTILKLIG
C0PQ13_______________FQAVELWVADRAVLPVENSLGGSIHRNYDLLLRHRLHIVGEVQLRVHHCLMALPGTRKEDLRRxxSHPQALAQCEHTISKLVG

SNPs (tot: 12)

Position	A %	C %	G %	T %	Probability
93			20	80	0.995498
96		80	20		0.995498
99	20			80	0.995498
103		75		25	0.996473
111			75	25	0.996473
112		25	75		0.996473
116			75	25	0.996473
118	25	75			0.996473
166		75		25	0.996473
207	25	75			0.996473
249		33		66	0.997452
391	66	33			0.997476

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain