SustainPineDB

All assemblies
Assembly info
Unigenes
SNPs
SSRs
Descriptions
GOs
ECs
KEGGs
InterPros
miRNAs

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene65227

Length: 492 nt

Origin of reads: Biogeco_1 Uagpf

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>sp_v1.1_unigene65227
CGTCGTGACCTTGGCTGTCACTCAAATGCCGAAGGAAAAGAAGGCATGTGTGTTGATGGTGCCATAAAAAATTACACTTCTGAGCTTGGAAATTATGGTGGCGGAGCCACTGGTGTAAATTATAATTTAACAGAAGCTTTATTGTTCCTCTCTCATTTTATGGGAGATATCCATCAGCCTTTACATGTGGGTTTCACTACAGATGAAGGTGGAAATACAATAAAATTACACTGGTATGGGCAACAGAGCAATTTGCATCATGTATGGGATAGTCTGATCATTGAGACAGCCATGAAAGATTACTATGATAATGACCTTGAGGAAATGATAGCAGCCATTCAGAAAAATGTTACCGGCAACTGGTCTGATGAAGTACCTACCTGGGAGAATTGCAGCACGGATGTACTTGCTTGCCCCAAACCGTATGCTGATGAAAGTATCAGTCTAGCTTGCAATTGGGCTTACAAAGACGCTGATGAAGACAGACGTTTT

Ace file of the UniGene sp_v1.1_unigene65227 (antisense)

If you push the Download ace button, you will download an ace file of this UniGene.

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Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	pco064225, nuc; LOC100170237	-	-	54%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	85%
Blast2go	bifunctional nuclease bfn1	-	-	76%

Source	ECs	Term	Type	e value	Identity
Blast2go	Hydrolases, Acting on ester bonds, Endoribonucleases producing 5'-phosphomonoesters.	EC:3.1.26.-	-	0.0	76%

Source	GOs	Term	Type	Identity
Blast2go	single-stranded DNA specific endodeoxyribonuclease activity	GO:0000014	Molecular Function	76%
Blast2go	endoribonuclease activity, producing 5'-phosphomonoesters	GO:0016891	Molecular Function	76%
Blast2go	T/G mismatch-specific endonuclease activity	GO:0043765	Molecular Function	76%

Source	InterPros	Term	Type	e value	Identity
Blast2go	S1/P1 nuclease	IPR003154	-	0.0	76%
Blast2go	Phospholipase C/P1 nuclease domain	IPR008947	-	0.0	76%

Full-Lengther Next Prediction

Fln status: Internal

Fln database: coniferopsida.fasta

Fln subject: B8LP38

Fln msg: Distance to subject end: 34 aas, your sequence is shorter than subject: 164 - 294

Fln protein: RRDLGCHSNAEGKEGMCVDGAIKNYTSELGNYGGGATGVNYNLTEALLFLSHFMGDIHQPLHVGFTTDEGGNTIKLHWYGQQSNLHHVWDSLIIETAMKDYYDNDLEEMIAAIQKNVTGNWSDEVPTWENCSTDVLACPKPYADESISLACNWAYKDADEDRRF

Protein Length: 165

Fln nts: CGTCGTGACCTTGGCTGTCACTCAAATGCCGAAGGAAAAGAAGGCATGTGTGTTGATGGTGCCATAAAAAATTACACTTCTGAGCTTGGAAATTATGGTGGCGGAGCCACTGGTGTAAATTATAATTTAACAGAAGCTTTATTGTTCCTCTCTCATTTTATGGGAGATATCCATCAGCCTTTACATGTGGGTTTCACTACAGATGAAGGTGGAAATACAATAAAATTACACTGGTATGGGCAACAGAGCAATTTGCATCATGTATGGGATAGTCTGATCATTGAGACAGCCATGAAAGATTACTATGATAATGACCTTGAGGAAATGATAGCAGCCATTCAGAAAAATGTTACCGGCAACTGGTCTGATGAAGTACCTACCTGGGAGAATTGCAGCACGGATGTACTTGCTTGCCCCAAACCGTATGCTGATGAAAGTATCAGTCTAGCTTGCAATTGGGCTTACAAAGACGCTGATGAAGACAGACGTTTT

Fln Alignment:

sp_v1.1_unigene65227___CHSNAEGKEGMCVDGAIKNYTSELGNYGGGATGVNYNLTEALLFLSHFMGDIHQPLHVGFTTDEGGNTIKLHWYGQQSNLHHVWDSLIIETAMKDYYDNDLEEMIAAIQKNVTGNWSDEVPTWENCSTDVLACPKPYADESISLACNWAYKDADED
B8LP38_______________CH-NPEGEEGMCVDGAIKNYTSQLGNYGCRATGANYNLTEALLFLSHFMGDIHQPLHVGFSTDEGGNTIKLHWYGRQNNLHHIWDTLIIETAMKDNYNNDLEEMIAAIQKNVTDSWSDEVPTWEKCSTNALACPKLYADESINLACNWAYKDADQN

SNPs (tot: 12)

Position	A %	C %	G %	T %	Probability
190		20	80		0.995493
191		20		80	0.995493
192	80	20			0.995493
205			50	50	1.0
344	75			25	0.996473
347		25	75		0.996473
348	25		75		0.996473
349	75		25		0.996473
350	75	25			0.996473
357	75	25			0.996473
359	75	25			0.996473
363			25	75	0.996473

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain