SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene64367

Length: 841 nt

Origin of reads: Biogeco_1 Biogeco_2 Uagpf Uma

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v1.1_unigene64367 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	DUF1084 multi-domain protein	-	-	71%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	44%
Blast2go	virion binding	-	-	84%

Full-Lengther Next Prediction

Fln status: C-terminus

Fln database: coniferopsida.fasta

Fln subject: B8LQF7

Fln msg: Overlapping hits, possible frame ERROR between 259 and 144, your sequence is shorter than subject: 255 - 280

Fln protein: GLVAAVALIQLIRIELRVPEYGWTTQKVFIFLMLL*MEVSVGQRLKLxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxFWAEIYYQARAISVDGYRPAFITINGIIYLIQICIWIYSWWSPSPVVEILSKLFFAGVSFFAALGFLLYGGRLFFMLRRFPVESTGRRKKLQEVGYVTTICFTCFFIRCVMMFFSAFDKGANLDVLDHPILNFMYYLIVEIVPSSLVLIILRKLPPKRGTAHYHPIR*

Protein Length: 256

Fln nts: CGGACTAGTTGCGGCAGTTGCTCTTATTCAACTTATAAGGATTGAGCTCAGAGTGCCAGAATATGGGTGGACAACGCAGAAAGTTTTCATTTTCTTAATGTTGTTGTGAATGGAGGTAAGCGTCGGACAAAGACTAAAACTCxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxTTTTGGGCAGAAATATACTATCAGGCACGGGCAATATCAGTAGACGGTTATAGACCCGCTTTTATCACAATCAATGGTATCATCTATCTCATTCAGATATGCATATGGATTTACTCTTGGTGGAGCCCTAGTCCAGTTGTTGAGATCCTCTCCAAGTTATTCTTTGCAGGTGTCTCCTTCTTTGCAGCGTTGGGGTTTTTACTTTATGGTGGCAGACTGTTCTTCATGCTTCGACGTTTTCCAGTTGAATCAACAGGACGCAGAAAGAAGTTGCAAGAGGTCGGCTATGTTACTACAATCTGCTTTACTTGTTTTTTCATAAGATGCGTCATGATGTTTTTCTCTGCATTTGACAAAGGTGCAAACCTTGATGTTTTGGATCATCCGATCTTGAACTTCATGTATTACTTGATAGTGGAAATAGTTCCATCTTCACTTGTTCTCATAATACTACGGAAATTGCCTCCAAAGCGTGGTACAGCTCATTATCATCCAATCCGTTGAAGTCACAAATGGTTTCAAGGAAAACAGAGTTTCCTGATCATTTGCGAATTCCATATGCCAGTGCATATTATCAAGAT

Fln Alignment:

sp_v1.1_unigene64367___GLVAAVALIQLIRIELRVPEYGWTTQKVFIFLMLL*MEVSxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxDGYRPAFITINGXXXXXXXXXXIYSWWSPSPVVEILSKLFFAGVSXXXXXXXXXXXXXXXXXXXXFPVESTGRRKKLQEVGYVTTICFTCFFIRCVMMFFSAFDKGANLDVLDHPILNFMYYLIVEIVPSSLVLIILRKLPPKRGTAHYHPIR
B8LQF7_______________GFVAAVALIQLIRIELRVPEYGWTTQKVFHFLNVVVNGVRxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxDGYRPAFMTINGIIYLIQICIWIYSWWSPSPVVEILSKLFFAGVSFFAALGFLLYGGRLFLMLRRFPVESTGRRKKLQEVGYVTTICFTCFFIRCVMMFFSAFDKGANLDVLDHPILNFMYYLLVEIVPSSLVLIILRKLPPKRGTAQYHPIR

SNPs (tot: 70)

Position	A %	C %	G %	T %	Probability
431	90			10	0.90298
434	90		10		0.90298
436	90		10		0.90298
441	10		90		0.90298
442		10	90		0.90298
444	10		90		0.90298
447		10	90		0.90298
449			10	90	0.90298
451	10			80	0.959698
453	90		10		0.90298
455		90	10		0.90298
456	80		20		0.999996
457	88			11	0.923564
459	11			88	0.923564
460		11	77		0.968132
463		88	11		0.923564
464			11	88	0.923564
466		12	87		0.992554
467	12		87		0.992554
472	12	87			0.992554
475		87	12		0.992554
478	87	12			0.992554
489		90		10	0.90298
490	90		10		0.90298
491	10			90	0.90298
492	90		10		0.90298
497		10		90	0.90298
499			10	90	0.90298
502		10	90		0.90298
509	90			10	0.90298
510		10		90	0.90298
514		10		90	0.90298
518	77		22		0.999999
519	22	77			0.999999
522			22	77	0.999999
523		22		77	0.999999
524		22	77		0.999999
526	22			77	0.999999
527			22	77	0.999999
530		22	77		0.999999
533	77	22			0.999999
534	50		50		1.0
536	77	22			0.999999
573		28		71	1.0
617	16		83		0.994512
659		80		20	0.995493
660	80		20		0.995493
662	80	20			0.995493
664			20	80	0.995493
666		20	80		0.995493
667	20	80			0.995493
673	20			80	0.995493
674		20		80	0.995493
675			20	80	0.995493
677		20		80	0.995493
710	25	75			0.996473
715		50		50	1.0
718	50		50		1.0
719		50		50	1.0
720			50	50	1.0
721	50			50	1.0
722		50		50	1.0
725		50		50	1.0
726	50	50			1.0
727	50			50	1.0
728	50			50	1.0
729			50	50	1.0
730	50		50		1.0
735	50		50		1.0
737	50	50			1.0

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain