SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene64261

Length: 1467 nt

Origin of reads: Biogeco_1 Biogeco_2 Uagpf Uma

UniGene Fasta

Ace file of the UniGene sp_v1.1_unigene64261

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Adenylosuccinate synthetase 2, chloroplastic n=1 Tax=Ricinus communis RepID=PURA2_RICCO	-	-	81%
FL-Next	sp=Adenylosuccinate synthetase; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	95%
Blast2go	adenylosuccinate synthetase	-	-	85%

Source	ECs	Term	Type	e value	Identity
Blast2go	Adenylosuccinate synthase.	EC:6.3.4.4	-	0.0	85%

Source	KEGGs	Term	Identity
Blast2go	Purine metabolism	00230	85%
Blast2go	Alanine, aspartate and glutamate metabolism	00250	85%
Blast2go	Biosynthesis of alkaloids derived from histidine and purine	01065	85%
Blast2go	Metabolic pathways	01100	85%

Source	GOs	Term	Type	Identity
Blast2go	AMP biosynthetic process	GO:0006167	Biological Process	85%
Blast2go	response to cadmium ion	GO:0046686	Biological Process	85%
Blast2go	adenylosuccinate synthase activity	GO:0004019	Molecular Function	85%
Blast2go	chloroplast stroma	GO:0009570	Cellular Component	85%
Blast2go	apoplast	GO:0048046	Cellular Component	85%

Source	InterPros	Term	Type	e value	Identity
Blast2go	Adenylosuccinate synthetase	IPR001114	-	0.0	85%
Blast2go	Adenylosuccinate synthase, active site	IPR018220	-	0.0	85%

Full-Lengther Next Prediction

Fln status: N-terminus

Fln database: coniferopsida.fasta

Fln subject: D5A8A1

Fln msg: Unexpected STOP codon at 3' end. Distance to subject end: 25 aas, your sequence is longer than subject: 445 - 309

Fln protein: MASALLRDLCVPHLHGFSQDKKKRQQGLRSGFAYTVNLHLAKGRIVLQSRRIKCNASVVEADAIVEAEKAEDPFKSLSQVCAVLGTQWGDEGKGKLVDILAQRYDIVARCQGGANAGHTIYNSEGKKFALHLVPSGILNEKALCVIGNGAVVHLPGLFEEIDGLESNGVSCKGRLLISDRAHLLFDFHQTIDGMREAELAGSLIGTTKRGIGPCYASKAIRNGIRVSDLRHMDTFPEKLDILLHDAASRFKGFEYSKSMLQTEVEHYKRYSERIEPYIADTVHLMNDAFSSGKKILVEGGQATMLDVDFGTYPFVTSSNPSAGGICTGLGIAPRHIGDMIGVAKAYTTRVGSGPYPTELLDEAGEDLRSAGHEYGTTTGRPRRCGWLDLVALKYSCQINGFSALNLTKLDVLSGLSRIRLGVAYKGPDGNVLKSFPADLDLLTP*

Protein Length: 446

Fln nts: ATGGGGGAAGGTTGTGACTTGGCTGTCTGTGGAATAA_-_ATGGCATCTGCGCTGCTTCGTGATCTTTGCGTTCCTCATTTGCACGGATTTTCGCAGGATAAGAAGAAGAGGCAGCAGGGGTTGCGGTCAGGGTTTGCGTATACTGTGAATTTGCATTTGGCTAAAGGTCGAATTGTACTGCAGAGTAGAAGGATAAAATGCAATGCCAGCGTCGTGGAGGCCGACGCCATTGTTGAGGCAGAGAAAGCGGAAGATCCTTTTAAATCCCTGAGTCAAGTGTGTGCAGTGCTGGGAACTCAGTGGGGTGACGAAGGAAAGGGCAAGCTCGTTGACATTTTAGCACAGCGCTATGATATTGTGGCCCGTTGTCAGGGAGGGGCAAATGCAGGTCACACCATATACAATAGCGAGGGAAAGAAATTTGCTCTTCACCTTGTCCCTTCTGGAATACTGAATGAGAAAGCCCTATGCGTTATTGGGAATGGAGCCGTTGTGCATCTTCCAGGACTATTTGAAGAGATAGACGGGCTTGAGTCTAATGGAGTTTCTTGCAAAGGAAGACTGTTGATTTCTGACCGTGCTCATTTACTATTTGATTTCCATCAAACTATTGATGGAATGAGAGAAGCAGAACTTGCAGGGTCGTTAATTGGAACGACAAAAAGAGGGATTGGTCCTTGCTATGCAAGCAAGGCAATCCGGAATGGTATTCGGGTAAGTGATTTGAGGCACATGGATACATTTCCTGAGAAGTTGGATATTCTTTTGCATGATGCCGCATCCCGTTTTAAAGGCTTTGAGTATAGTAAAAGCATGTTACAGACTGAAGTGGAGCATTACAAGAGATATTCTGAGCGAATAGAACCTTATATTGCTGATACTGTTCATTTGATGAATGATGCATTCTCAAGCGGAAAGAAGATTTTGGTTGAAGGCGGCCAGGCGACCATGTTAGATGTCGATTTTGGAACTTATCCATTTGTTACTTCTTCTAATCCATCTGCCGGTGGAATCTGTACTGGACTTGGTATTGCTCCAAGACATATTGGTGACATGATTGGTGTGGCTAAAGCGTACACAACAAGAGTTGGTTCAGGTCCTTACCCGACAGAACTCTTGGATGAAGCGGGTGAAGATCTTCGATCAGCTGGACATGAATATGGCACCACAACTGGACGGCCTCGTCGTTGTGGTTGGCTAGATCTGGTTGCACTTAAATATTCTTGTCAAATCAATGGTTTCTCAGCACTTAATCTGACAAAGCTTGACGTACTTTCTGGGTTATCTCGAATTAGGCTTGGGGTAGCCTATAAAGGGCCTGATGGAAATGTGTTGAAATCTTTTCCTGCGGACCTTGACCTTCTTACACCTTAGCGCACACAGGAGGTAGGGATTCAGCGCCGTGAGCGGGAGTGGTGACTGCTAAAGGGTTGCTGAGGACCTGCGAGGTGAGCCAAGGAGCGCCCCAT

Fln Alignment:

sp_v1.1_unigene64261___MREAELAGSLIGTTKRGIGPCYASKAIRNGIRVSDLRHMDTFPEKLDILLHDAASRFKGFEYSKSMLQTEVEHYKRYSERIEPYIADTVHLMNDAFSSGKKILVEGGQATMLDVDFGTYPFVTSSNPSAGGICTGLGIAPRHIGDMIGVAKAYTTRVGSGPYPTELLDEAGEDLRSAGHEYGTTTGRPRRCGWLDLVALKYSCQINGFSALNLTKLDVLSGLSRIRLGVAYKGPDGNVLKSFPADLDLL
D5A8A1_______________MREAELAGSLIGTTKRGIGPCYASKVIRNGIRVSDLRHMDTFPEKLDILLHDAASRFKGFEYSKSMLQTEVEHYKRYSERVEPYIAVTVHLMHDAFSSGKRILVEGGQATMLDVDFGTYPFVTSSNPSAGGICTGLGIAPRHIGDLIGVAKAYTTRVGSGPYPTELLDEAGENLRSAGYEYGTTTGRPRRCGWLDLVALKYSCQINGFSTLNLTKLDVLSGLSRIRLGVAYKGPDGNVLPSFPADLDLL

SNPs (tot: 16)

Position	A %	C %	G %	T %	Probability
353	11	88			0.991528
356	88		11		0.991528
357			11	88	0.991528
363		11		88	0.923564
556		80		20	0.995493
561	80		20		0.995493
626	16		83		0.994512
897	80		20		0.995493
900			20	80	0.995493
904		20		80	0.995493
905		20	80		0.995493
906	80			20	0.995493
907	80			20	0.995493
908			20	80	0.995493
910	80	20			0.995493
985		20		80	0.995493

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain