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Fln status: Putative N-terminus

Fln database: coniferopsida.fasta

Fln subject: D5AD86

Fln msg: Separated hits, possible frame ERROR between 296 and 326, and overlapping frame ERROR between 406 and 388, Distance to subject end: 202 aas, atg_distance in limit (1-15): atg_distance = 5, Unexpected STOP codon in 5 prime region, your sequence is shorter than subject: 174 - 514

Fln protein: PWLSLIFPIVLSASANLTALEHGKQVHGYIIRAGTESNVFVASALVHMYGKCGNSEDAKMVFDNMDERNVVSWNTIISGYAQNGPAHEALKLFEQxxxxxxxxxxxNFYGVLTACCHRGLVAEGCxxxxxxxxxLCIAPREDHYACMVDLYSRAGRLNEAEKLINDMPFEPDAV

Protein Length: 175

Fln nts: CGTCGTGACCTTGGCTGTCACTCATTTTTCCTATCGTACTTAGTGCTTCTGCCAATCTTACAGCTTTGGAACATGGCAAGCAAGTCCATGGATACATTATCAGGGCTGGAACTGAGTCAAATGTGTTTGTGGCAAGTGCCCTTGTCCACATGTATGGCAAATGTGGAAACAGCGAGGATGCAAAAATGGTGTTCGATAACATGGATGAGCGCAATGTGGTGTCATGGAATACTATTATTTCAGGATATGCCCAGAATGGGCCAGCCCACGAGGCTCTCAAACTCTTTGAACAGATxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxTAACTTTTATGGTGTTTTAACTGCATGTTGCCATCGAGGCCTGGTGGCTGAAGGTTGCTCxxxxxxxxxxxxxxxxxxxxxxxxCCTCTGCATTGCTCCAAGAGAAGATCACTATGCATGCATGGTTGATCTTTATAGCCGTGCCGGCCGCTTGAATGAAGCAGAGAAGTTGATCAACGACATGCCATTTGAACCGGATGCTGTTA

Fln Alignment:

sp_v1.1_unigene62739___VLSASANLTALEHGKQVHGYIIRAGTESNVFVASALVHMYGKCGNSEDAKMVFDNMDERNVVSWNTIISGYAQNGPAHEALKLFEQMIQAGQKPNHVTFMxxxxxxxxxxNFYGVLTACCHRGLVAEGCSYxxxxxxSLCIAPREDHYACMVDLYSRAGRLNEAEKLINDMPFEPD
D5AD86_______________VLSACADLAALELGRQFHAYVVQSGFALDIVVGSALVDMYAKSGSMEDACQVFDKMPQRNEVSWNSIITGCAQHGRGNDAVLLFEQMLQAGIKPNEISFVxxxxxxxxxxSFVGVLSACSHTGLVNEGRGYxxxxxxNYGIVPDVSHYTCMIDLLGRAGCLDEAENFINGMPVEPD