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Fln status: Putative C-terminus

Fln database: coniferopsida.fasta

Fln subject: B8LLF5

Fln msg: Overlapping hits, possible frame ERROR between 798 and 798, and possible frame ERROR between 941 and 944, Unexpected stop codon in the beginning of your sequence, STOP codon was not found. Distance to subject end: 6 aas, your sequence is shorter than subject: 339 - 500

Test code: 0

Test Code was used to find complete genes when there was not found a reliable orthologue. The best ORF (Open Reading Frame) is shown, and only ORFs > 200pb were analyzed. Your ORF will be more reliable if a stop codon was found before the start codon. A Test Code value > 0.95 means the ORF is probably coding. A Test Code value < 0.74 means the ORF is probably non-coding. Test Code values in between 0.74 and 0.95 mean it is uncertain whether the ORF is coding or not.

Fln protein: ELSTHNVCS*DQSCLACGCTIVIKSAEQTPLVALYCAHLAKEAGIPPGVLNVLSGFGETAGAAISSHMDIDKIAFTGSTEVGRLIMEASAKSNLKPVTLELGGKSPLIIMDDANVDEAVDLAHLAVYVNMGQVCCAGSRVFVQEGIYDEFLKKAVARAKQQVVGDPFKPGVHQGPQIDKVQYEKVLEYIQYGKREGANLLTGGNHLGDKGFYIEPTIFSDVKDDMKIAQEEIFGPVMSILKFKTIEEVIERGNKTIYGLSAGIVTxxINVANRLSRSLCAGVVWINCYLVFSPDCPFGGYKMSGIGREYGSYSxxNYLQVKCVITLFKSHLGCKSIVSS

Protein Length: 340

Fln nts: GAACTATCCACTCACAATGTTTGTTCTTAAGATCAGTCCTGCCTGGCCTGTGGATGTACAATAGTCATTAAATCTGCAGAGCAGACTCCCTTAGTCGCATTATATTGTGCCCACTTGGCCAAAGAGGCTGGCATACCGCCTGGTGTGCTTAATGTGCTGTCTGGCTTTGGAGAAACGGCTGGGGCTGCCATAAGCAGTCATATGGATATCGATAAGATTGCATTTACAGGGTCAACTGAAGTTGGGCGACTTATCATGGAAGCTTCTGCAAAAAGCAATCTGAAGCCCGTGACATTAGAGCTGGGTGGAAAATCTCCTCTCATAATCATGGATGACGCTAATGTTGATGAAGCAGTGGATCTTGCCCATCTTGCAGTATACGTTAACATGGGTCAAGTATGTTGTGCAGGGTCGAGGGTGTTTGTTCAAGAGGGCATCTATGATGAATTTCTGAAGAAAGCGGTGGCAAGGGCCAAGCAACAAGTGGTGGGGGACCCTTTCAAGCCTGGAGTTCATCAAGGTCCTCAGATTGATAAGGTGCAATATGAAAAAGTACTAGAGTACATTCAATATGGAAAAAGAGAAGGAGCTAATTTATTAACAGGTGGTAATCATTTAGGTGATAAAGGATTTTACATTGAACCTACAATATTCTCCGACGTCAAGGATGATATGAAAATTGCACAAGAAGAAATATTTGGACCAGTTATGTCCATCTTAAAATTCAAGACCATAGAAGAGGTAATAGAGAGAGGAAACAAAACAATATATGGTTTAAGTGCAGGGATTGTTACTxxxxxxATAAATGTAGCCAATAGACTCTCTAGATCTCTTTGTGCAGGAGTGGTATGGATCAATTGTTACCTTGTATTTAGTCCAGATTGTCCTTTTGGAGGATATAAGATGAGTGGAATAGGACGAGAGTATGGGTCATATAGTCxxxGAAATTACTTACAAGTCAAGTGTGTTATCACCCTCTTCAAGAGTCACCTTGGTTGTAAGAGTATAGTATCATCA

Fln Alignment:

sp_v1.1_unigene61013___LACGCTIVIKSAEQTPLVALYCAHLAKEAGIPPGVLNVLSGFGETAGAAISSHMDIDKIAFTGSTEVGRLIMEASAKSNLKPVTLELGGKSPLIIMDDANVDEAVDLAHLAVYVNMGQVCCAGSRVFVQEGIYDEFLKKAVARAKQQVVGDPFKPGVHQGPQIDKVQYEKVLEYIQYGKREGANLLTGGNHLGDKGFYIEPTIFSDVKDDMKIAQEEIFGPVMSILKFKTIEEVIERGNKTIYGLSAGIVTKNINVANRLSRSLCAGVVWINCYLVFSPDCPFGGYKMSGIGREYGSYSLxNYLQVKCVIT
B8LLF5_______________LACGCTVVIKSAEQTPLTALYCAQLANEAGIPPGVLNVLSGFGETAGASISSHMDIDKISFTGSTQVGRLIMEAAAKSNLKPVTLEMGGKSPLIVMDDADVDKAVNIAHLAVYTNMGQVCLAGSRVFVQEGIYDEFVKKAVARAKQQVVGDPFQPGVQHGPQIDKMQFEKILKYIQYGKRDGANLVLGGNSLGNKGFYIEPTIFSDVEDDMQIAKEEIFGPIMSILKFKTIEEVIERGNKTIYGLGAGIITKDIDIANRLARSLRVGTVWINCYLVVGADVPLGGYKMSGIGREYGSYGLxNYLQVKCVIS