SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene60019

Length: 710 nt

Origin of reads: Biogeco_1 Biogeco_2 Uagpf Uma

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v1.1_unigene60019 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Clathrin assembly protein, putative n=1 Tax=Ricinus communis RepID=B9T128_RICCO	-	-	88%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	97%
Blast2go	protein	-	-	90%

Source	GOs	Term	Type	e value	Identity
Blast2go	binding	GO:0005488	Molecular Function	0.0	90%
Blast2go	plasma membrane	GO:0005886	Cellular Component	0.0	90%

Source	InterPros	Term	Type	Identity
Blast2go	ENTH/VHS	IPR008942	-	90%
Blast2go	ANTH	IPR011417	-	90%
Blast2go	Epsin-like, N-terminal	IPR013809	-	90%

Full-Lengther Next Prediction

Fln status: N-terminus

Fln database: coniferopsida.fasta

Fln subject: B8LKD7

Fln msg: Overlapping hits, possible frame ERROR between 587 and 587, Distance to subject end: 352 aas, your sequence is shorter than subject: 194 - 547

Test code: 1

Test Code was used to find complete genes when there was not found a reliable orthologue. The best ORF (Open Reading Frame) is shown, and only ORFs > 200pb were analyzed. Your ORF will be more reliable if a stop codon was found before the start codon. A Test Code value > 0.95 means the ORF is probably coding. A Test Code value < 0.74 means the ORF is probably non-coding. Test Code values in between 0.74 and 0.95 mean it is uncertain whether the ORF is coding or not.

Fln protein: MSGGGTQQSLRKALGALKDTTTVSLAKVNSDYKDLDIAIVKATNHVERPAKEKHIRIIFAATSAARPRADVAYCIHALARRLAKTHNWAVALKTLIVIHRALREVDPTFREELINYSRSRGHILNLSYFKDDSSPNAWDYSAWVRAYALFLExxLECYRVLKYDIETERLRTRELDTPELLEQLPALQQYLYRL

Protein Length: 195

Fln nts: CGTCGTGACCTTGGCTGTCACTCAACTTGATCTGGAGATCTGCACCTGCGGAAACACAGGCACTCGCTTTTTACAGATCTTCAAAGAGACCAATTTGATTGCAGATCTTCAAAGATTAGACCTGTACA_-_ATGTCAGGCGGTGGCACACAACAGAGCCTGAGGAAGGCATTGGGTGCACTGAAAGATACAACGACAGTTAGTCTTGCCAAAGTGAACAGTGATTACAAGGATCTAGACATTGCTATTGTGAAGGCCACAAATCATGTTGAGCGGCCAGCAAAAGAAAAGCATATCCGAATTATTTTTGCAGCAACATCGGCTGCAAGGCCTCGGGCAGATGTGGCATATTGCATCCATGCTCTTGCAAGACGTCTTGCCAAGACTCACAACTGGGCGGTTGCGTTAAAAACCTTAATTGTTATCCATCGAGCTTTGAGAGAGGTTGACCCCACGTTTCGGGAGGAATTGATCAATTATAGTAGATCCAGAGGCCACATTCTCAATTTATCTTACTTCAAGGATGATTCAAGTCCAAATGCTTGGGACTATTCAGCGTGGGTACGTGCATATGCATTGTTCCTGGAAxxxxxxTTGGAATGCTACCGTGTACTGAAATATGACATTGAAACGGAGCGCTTGAGAACCAGGGAACTGGACACTCCTGAATTGCTAGAACAATTGCCAGCTTTGCAGCAATATCTTTATCGTCTAA

Fln Alignment:

sp_v1.1_unigene60019___MSGGGTQQSLRKALGALKDTTTVSLAKVNSDYKDLDIAIVKATNHVERPAKEKHIRIIFAATSAARPRADVAYCIHALARRLAKTHNWAVALKTLIVIHRALREVDPTFREELINYSRSRGHILNLSYFKDDSSPNAWDYSAWVRAYALFLEDRLECYRVLKYDIETERLRTRELDT
B8LKD7_______________MSGGGTQQSLRKALGALKDTTTVSLAKVNSDYKDLDIAIVKATNHVERPAKEKHIRIIFAATSATRPRADVAYCIHALARRLAKTHNWAVALKTLIVIHRALREVDPTFREELINYSRSRGHILNLSYFKDDSSSNAWDYSAWVRSYALFLEERLECYRVLKYDIETERLRTRELDT

SNPs (tot: 39)

Position	A %	C %	G %	T %	Probability
429	10			90	0.903027
430	10			90	0.902982
431			10	90	0.902982
432		90	10		0.90298
434		10		90	0.902989
435	10			90	0.902989
436		10		90	0.902989
456			10	90	0.90298
457	10			90	0.90298
461	10		90		0.90298
463	10	90			0.90298
464			10	90	0.90298
465		10		90	0.90298
468	10	90			0.90298
469	90		10		0.90298
470	90		10		0.90298
472	90	10			0.90298
473	10		90		0.90298
475	90		10		0.90298
477		10		90	0.90298
528		12	87		0.992554
529		12		87	0.992554
578	16		83		0.994512
579		83		16	0.994512
598	75			25	0.996474
604	25	25		50	0.995551
605		25	75		0.996473
606	25			25	0.995595
607	75			25	0.99649
608	50			25	0.995533
609	25		50	25	0.995533
610	75			25	0.996473
611	25			50	0.99554
612		75		25	0.996473
613	25			75	0.996476
617	66		33		0.997457
618		33	33	33	0.997752
624		33		66	0.997452
625		33	66		0.997461

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain