SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene59940

Length: 1248 nt

Origin of reads: Biogeco_1 Uagpf Uma

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v1.1_unigene59940 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Nuclease PA3, putative (EC:3.1.30.1)	-	-	58%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	75%
Blast2go	bifunctional nuclease	-	-	74%

Source	GOs	Term	Type	e value	Identity
Blast2go	single-stranded DNA specific endodeoxyribonuclease activity	GO:0000014	Molecular Function	0.0	74%
Blast2go	T/G mismatch-specific endonuclease activity	GO:0043765	Molecular Function	0.0	74%

Source	InterPros	Term	Type	e value	Identity
Blast2go	S1/P1 nuclease	IPR003154	-	0.0	74%
Blast2go	Phospholipase C/P1 nuclease domain	IPR008947	-	0.0	74%

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: B8LP38

Fln msg:

Fln protein: MGWFGCSMLVGIGMEILILLLRVGMAPHGVEAWGNEGHYSICKIAEPLLSEEASIAVKKLLPEDAEGDLASLCSWADHMRFHYPWSRPLHFIDTQDNKCSYVYSRDCHNAEGEIGMCLEGAIKNYTSQLETYGSRLDSEKYNLTEALLFLSHFIGDVHQPLHVGFSTDEGGNTIILHWYRQKSNLHHIWDTLMIETAMKDFYDDDLEVMISSIQRNITDSWSDEVPTWEKCNGHDLACPKVYADESIILACKWAYKDAEANSVLEDDYFLSRLPIVQTQLAKAGVRLAATLNRLFNSKEGYNRKSEL*

Protein Length: 309

Fln nts: ATGGGGAACAGAGTCGGCGTTTCCTGTGTGAATGTTGCAGTAGCTACGGGATCAACAGGTTGCAACGTAAAATATTTTGGGAGAGTTACAGAGCATTGGAGAGGTTTTGGTGATA_-_ATGGGTTGGTTTGGGTGTTCAATGTTGGTTGGAATAGGAATGGAGATATTGATCCTCCTCCTCCGTGTTGGTATGGCTCCTCATGGTGTGGAAGCTTGGGGCAATGAAGGACACTATTCGATTTGTAAGATAGCTGAGCCACTCCTAAGTGAAGAAGCATCAATAGCAGTGAAAAAATTGCTCCCTGAGGATGCAGAGGGTGATCTTGCTTCTCTTTGTTCGTGGGCTGATCATATGCGATTCCACTATCCCTGGTCACGTCCATTACACTTCATTGACACGCAAGACAATAAGTGTTCTTATGTTTATTCCAGGGACTGCCATAATGCCGAAGGAGAAATAGGCATGTGCCTTGAAGGTGCCATAAAAAATTACACTTCTCAGCTTGAAACTTATGGCAGCAGACTCGACAGTGAAAAATATAATTTAACAGAAGCTTTACTATTTCTCTCTCATTTTATAGGAGATGTCCATCAGCCTCTACATGTGGGATTCAGTACAGATGAAGGTGGAAACACAATCATATTACACTGGTACAGGCAAAAGAGTAATTTGCATCATATATGGGATACACTGATGATCGAGACAGCAATGAAAGATTTTTATGATGATGACCTTGAGGTTATGATCTCATCAATTCAGAGAAATATTACTGATAGCTGGTCTGATGAAGTACCTACATGGGAGAAATGCAATGGGCATGATCTAGCCTGCCCCAAAGTGTATGCAGATGAAAGTATCATTCTTGCTTGCAAGTGGGCTTATAAAGACGCTGAAGCAAACAGTGTTCTAGAAGATGACTACTTCCTCTCTCGCTTGCCGATTGTTCAGACGCAACTTGCAAAAGCAGGTGTCAGGTTGGCTGCAACACTCAACCGATTATTCAATTCAAAAGAAGGCTACAATCGGAAATCAGAACTCTAG___AAAGAAAAGTGATGGGTTGCTTGCAACAATAGTATTGTAATCTATATTTAGATCAGAAACAGTGCTTGCGCCACTGAAAACAATAAGCGCTGTCACAAAATCTTGATTGTTTTTAAATCAATTGTAGCTTAACCCAGAGGAGCATTGGTATGAAGGATTCATGTATCATAAAGATGTCGTGTGGTTCTGTTTCCACAGCCATTTTAATG

Fln Alignment:

sp_v1.1_unigene59940___LLLRVGMAPHGVEAWGNEGHYSICKIAEPLLSEEASIAVKKLLPEDAEGDLASLCSWADHMRFHYPWSRPLHFIDTQDNKCSYVYSRDCHNAEGEIGMCLEGAIKNYTSQLETYGSRLDSEKYNLTEALLFLSHFIGDVHQPLHVGFSTDEGGNTIILHWYRQKSNLHHIWDTLMIETAMKDFYDDDLEVMISSIQRNITDSWSDEVPTWEKCNGHDLACPKVYADESIILACKWAYKDAEANSVLEDDYFLSRLPIVQTQLAKAGVRLAATLNRLFNS
B8LP38_______________VMIYLSMVPTS-ESWGKEGHYATCKIAQPLLSEEASAAVKKLLPDYAEGDLASLCSWADQVRFRYRWASPLHFIDTPDNKCTYIYSRDCHNPEGEEGMCVDGAIKNYTSQLGNYGCRATGANYNLTEALLFLSHFMGDIHQPLHVGFSTDEGGNTIKLHWYGRQNNLHHIWDTLIIETAMKDNYNNDLEEMIAAIQKNVTDSWSDEVPTWEKCSTNALACPKLYADESINLACNWAYKDADQNSVLEDDYFFSRLPIVETQLAKGGVRLAATLNRIFNS

SNPs (tot: 12)

Position	A %	C %	G %	T %	Probability
735		0		92	1.0
780	2		98		0.900134
800		95		4	0.996252
833			93	6	0.999453
834		6	93		0.999453
1255	10		90		0.903175
1256	10	90			0.903175
1261	12			87	0.992554
1267	16			83	0.994512
1268	16	16			0.988813
1269	14	85			0.993541
1270	20	80			0.995505

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain