SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene59341

Length: 1661 nt

Origin of reads: Biogeco_1 Biogeco_2 Uagpf Uma

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v1.1_unigene59341 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Citrate synthase n=1 Tax=Picea sitchensis RepID=D5AAX0_PICSI	-	-	94%
FL-Next	sp=Citrate synthase; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	97%
Blast2go	protein	-	-	89%

Source	ECs	Term	Type	e value	Identity
Blast2go	Citrate (Si)-synthase.	EC:2.3.3.1	-	0.0	89%

Source	KEGGs	Term	Identity
Blast2go	Citrate cycle (TCA cycle)	00020	89%
Blast2go	Glyoxylate and dicarboxylate metabolism	00630	89%
Blast2go	Biosynthesis of phenylpropanoids	01061	89%
Blast2go	Biosynthesis of terpenoids and steroids	01062	89%
Blast2go	Biosynthesis of alkaloids derived from shikimate pathway	01063	89%
Blast2go	Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid	01064	89%
Blast2go	Biosynthesis of alkaloids derived from histidine and purine	01065	89%
Blast2go	Biosynthesis of alkaloids derived from terpenoid and polyketide	01066	89%
Blast2go	Biosynthesis of plant hormones	01070	89%
Blast2go	Metabolic pathways	01100	89%
Blast2go	Biosynthesis of secondary metabolites	01110	89%

Source	GOs	Term	Type	Identity
Blast2go	fatty acid beta-oxidation	GO:0006635	Biological Process	89%
Blast2go	citrate (Si)-synthase activity	GO:0004108	Molecular Function	89%
Blast2go	peroxisome	GO:0005777	Cellular Component	89%

Source	InterPros	Term	Type	Identity
Blast2go	Citrate synthase-like	IPR002020	-	89%
Blast2go	Citrate synthase-like, core	IPR016141	-	89%
Blast2go	Citrate synthase-like, large alpha subdomain	IPR016142	-	89%
Blast2go	Citrate synthase-like, small alpha subdomain	IPR016143	-	89%
Blast2go	Citrate synthase active site	IPR019810	-	89%

Full-Lengther Next Prediction

Fln status: Putative Complete

Fln database: coniferopsida.fasta

Fln subject: D5AAX0

Fln msg: query STOP codon is far from subject stop. Distance to subject end: 20 aas, your sequence is shorter than subject: 490 - 509

Fln protein: MAPMEGFSEKSMAHSRLAVLSGHLSVPPVGSSLGIETSTVSVKLSGDLRGSLTVIDNRTGKKIEVEVSEGGTVRATDFKKLTVGGDDKGLKIYDPGYLNTAPVRSSISYIDGDEGILRYRGYPIEELAEKSSYLEVAYLLMYGNLPSQSQLADWEFNISQHSAAPEGVLDIIQAMPHDAHPMGMLVSAISALSTFHPDANPALRGQDLYNSKSIRDKQISRVLGKVPTIAAAAYLRMAGRPPVLPSSNLSYSENFLYMLDSLGDRSYKPNPRLARAIDVLFILHAEHEMNCSTAAARHLASSGVDVYTALAGATGALYGPLHGGANEAVLRMLNEIGTIDNIPEFIEGVKNRKRKMSGFGHRVYKNYDPRAKVIRNLAEEVFSIVGRDPLIEVAVALERAALSDEYFIKRKLYPNVDFYSGLIYRALGFPTEFFPVLFAIPRMAGYLAHWKESLDDPDTKIMRPQQVLRICYLWIAFTIFIHVLSSLMF*

Protein Length: 491

Fln nts: CGTCGTGACCTTGGCTGTCACTCAATTCCAATTTTTATATTTCCCCTGAACATTTTTTTTCTTCTGCTTGGGATCGGTTCAGAAGTGTATTGGGgACGTCCTGACCTTATCTGCCAAGTGTCAATCTTGAGGTGTTTTGGGGAATTGGGTTCGATCCAGTA_-_ATGGCACCTATGGAAGGTTTTAGTGAAAAATCGATGGCCCACAGCAGATTAGCAGTACTTTCTGGTCATTTATCGGTTCCTCCGGTGGGTTCTTCCTTAGGTATTGAGACTTCTACAGTGTCTGTAAAGCTCTCGGGGGATCTGAGAGGTTCTCTGACTGTTATTGATAACCGAACGGGGAAGAAAATTGAGGTCGAAGTCAGTGAGGGAGGAACAGTCCGGGCCACTGATTTCAAAAAGTTAACTGTAGGTGGGGATGACAAAGGACTGAAGATTTATGATCCAGGATACTTGAACACTGCTCCTGTCCGTTCATCAATATCTTACATAGATGGAGATGAAGGAATCCTGCGATATAGAGGTTATCCAATTGAGGAGTTAGCTGAGAAGAGCTCTTACCTTGAGGTGGCTTATCTCTTAATGTATGGAAATCTTCCTTCTCAGTCCCAGTTAGCAGATTGGGAATTCAATATTTCCCAACATTCAGCAGCGCCCGAGGGAGTATTGGATATCATACAAGCAATGCCTCATGATGCTCATCCAATGGGTATGCTTGTTAGTGCAATAAGTGCACTTTCTACATTTCATCCAGATGCCAATCCAGCATTAAGGGGACAAGATCTTTACAATTCAAAGTCCATTCGAGACAAGCAGATTTCACGTGTCTTGGGAAAGGTACCAACTATAGCAGCGGCTGCATACTTGAGAATGGCGGGGAGACCACCTGTCTTACCTTCCAGTAACCTTTCTTATTCAGAGAATTTTCTATATATGTTGGATTCACTGGGTGACAGATCATATAAACCCAATCCTCGACTAGCAAGAGCCATTGATGTACTTTTCATCTTGCATGCGGAGCATGAAATGAACTGTTCAACAGCTGCTGCACGTCACCTTGCTTCCAGTGGTGTTGATGTCTATACTGCTCTTGCTGGGGCGACTGGTGCGTTGTATGGTCCTTTGCATGGAGGGGCAAATGAGGCTGTTCTCAGAATGCTTAATGAAATTGGCACAATTGATAACATTCCAGAGTTCATTGAGGGTGTGAAAAACAGGAAAAGAAAAATGTCTGGTTTCGGTCATCGAGTATACAAGAATTATGATCCACGTGCTAAGGTTATTCGCAATTTAGCAGAAGAAGTGTTCTCTATTGTTGGACGTGATCCTTTGATTGAGGTGGCTGTGGCATTAGAGAGGGCTGCACTTTCAGATGAGTACTTTATTAAGAGGAAGCTCTACCCAAATGTTGATTTTTATTCAGGCTTAATTTACAGGGCACTGGGTTTCCCGACTGAATTCTTCCCAGTCCTATTTGCCATTCCACGTATGGCTGGTTATCTCGCTCATTGGAAGGAGTCACTTGATGATCCAGATACAAAAATCATGAGGCCACAACAGGTTCTTAGGATTTGTTATTTATGGATTGCGTTCACAATCTTCATTCATGTTTTAAGTTCATTGATGTTTTAA___ATTCTTTAAATTAATGCGTTTGTATCTTTC

Fln Alignment:

sp_v1.1_unigene59341___MAPMEGFSEKSMAHSRLAVLSGHLSVPPVGSSLGIETSTVSVKLSGDLRGSLTVIDNRTGKKIEVEVSEGGTVRATDFKKLTVGGDDKGLKIYDPGYLNTAPVRSSISYIDGDEGILRYRGYPIEELAEKSSYLEVAYLLMYGNLPSQSQLADWEFNISQHSAAPEGVLDIIQAMPHDAHPMGMLVSAISALSTFHPDANPALRGQDLYNSKSIRDKQISRVLGKVPTIAAAAYLRMAGRPPVLPSSNLSYSENFLYMLDSLGDRSYKPNPRLARAIDVLFILHAEHEMNCSTAAARHLASSGVDVXXXXXXXXXXXXGPLHGGANEAVLRMLNEIGTIDNIPEFIEGVKNRKRKMSGFGHRVYKNYDPRAKVIRNLAEEVFSIVGRDPLIEVAVALERAALSDEYFIKRKLYPNVDFYSGLIYRALGFPTEFFPVLFAIPRMAGYLAHWKESLDDPDTKIMRPQQ
D5AAX0_______________MAPVEGFDEKSMAHSRLAVLSGHLSVPPAGSSLGIETSTVSVKPSGNLRGSLTVIDNRTGRKIEVEVSEGGTVRATDFKKLTIGGDDKGLKLYDPGYLNTAPVRSSISYIDGDEGILRYRGYPIEELAEKSTYLEVAYLLMYGNLPSQSQLADWEFNVSQHSAAPEGVLDIIQAMPHDAHPMGMLVSAISALSTFHPDANPALRGQDLYNSKSIRDKQIARVLGKVPTIAAAAYLRMAGRPPVLPSSNLSYSENFLYMLDSLGDRSYKPNPRLARALDVLFILHAEHEMNCSTAAARHLASSGVDVYTALAGATGALYGPLHGGANEAVLRMLNEIGTVDNIPEFIEGVKNRKRKMSGFGHRVYKNYDPRAKVIRNLAEEVFSIVGRDPLIEVAVALERAALSDEYFIKRKLYPNVDFYSGLIYRALGFPTEFFPVLFAIPRMAGYLAHWKESLDDPDTKIMRPQQ

SNPs (tot: 11)

Position	A %	C %	G %	T %	Probability
1046	90	10			0.90298
1554	83			16	0.999991
1555		66	8	25	1.0
1556	75		25		1.0
1557	25	66			1.0
1558			41	58	1.0
1561	33		66		1.0
1565	63	36			1.0
1566		36		63	1.0
1574		36	63		1.0
1580	50	50			1.0

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain