SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene59318

Length: 1619 nt

Origin of reads: Biogeco_1 Biogeco_2 Uagpf Uma

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v1.1_unigene59318 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Heat shock cognate 70 kDa protein n=9 Tax=Magnoliophyta RepID=HSP7C_PETHY	-	-	94%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	92%
Blast2go	heat shock	-	-	96%

Source	InterPros	Term	Type	Identity
Blast2go	Heat shock protein Hsp70	IPR001023	-	96%
Blast2go	Heat shock protein 70	IPR013126	-	96%
Blast2go	Heat shock protein 70, conserved site	IPR018181	-	96%

Full-Lengther Next Prediction

Fln status: Putative Complete

Fln database: coniferopsida.fasta

Fln subject: B8LRY5

Fln msg: your sequence is shorter than subject: 414 - 652. Was predicted as Complete, but is very much shorter than de subject

Fln protein: MAGKGEGPAIGIDLGTTYSCVGVWQHDRVEIIANDQGNRTTPSYVGFTDTERLIGDAAKNQVAMNPTNTVFDAKRLIGRRFSDATVQSDIKMWPFKLTPGPADKPMIVVNYKGEEKQFAAEEISSMVLMKMREIAEAYLGVTIKNAVVTVPAYFNDSQRQATKDAGVIAGLNVMRIINEPTAAAIAYGLDKKAGSVGEKNVLIFDLGGGTFDVSLLTIEEGIFEVKSTAGDTHLGGEDFDNRMVNHFVQEFKRKFKKDISGSARALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYSTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSSIHDAIEQAIQWLDNNQLAEADEFDDKMKELESICNPIIAKMYQGAGGDVPGGGFGGVPPEGGPSSGGGAGPKIEEVD*

Protein Length: 415

Fln nts: CGTCGTGACCTTGGCTGTCACTGGGGAAGTTAGAAGGGTAGAGACTTTGCTGCTCTGCCTTTGAAGTATCCGTTGCATCTCTGTGTTATTTAATCGTTCTTTGAATTTGGGGGTAATATCTTCTGTAGAGTTTTGAA_-_ATGGCCGGCAAGGGAGAGGGCCCTGCGATCGGTATAGATCTCGGTACTACGTATTCCTGCGTTGGGGTGTGGCAGCATGACAGAGTGGAGATCATCGCCAATGACCAAGGGAACAGGACCACTCCCTCCTATGTGGGTTTCACTGATACTGAGCGACTCATTGGAGATGCCGCAAAGAATCAGGTCGCCATGAATCCCACCAATACCGTTTTTGATGCCAAGAGATTGATTGGAAGGCGTTTCAGTGACGCTACTGTTCAGAGTGACATCAAGATGTGGCCTTTTAAGTTAACACCGGGGCCTGCTGACAAGCCTATGATTGTTGTTAATTACAAAGGCGAGGAGAAGCAGTTTGCTGCAGAGGAGATTTCATCCATGGTGTTAATGAAGATGAGGGAGATTGCAGAGGCTTACCTTGGTGTCACAATAAAGAATGCCGTTGTCACTGTTCCTGCATATTTCAACGACTCCCAGAGGCAGGCTACCAAGGATGCTGGAGTGATTGCTGGGTTGAATGTGATGAGAATAATCAATGAGCCTACTGCTGCTGCTATTGCCTATGGTTTGGACAAGAAGGCTGGCAGTGTGGGTGAGAAAAATGTATTGATCTTTGATTTGGGGGGTGGAACATTTGATGTTTCGCTTTTAACTATTGAAGAGGGTATTTTTGAAGTGAAGTCTACAGCTGGTGACACCCATCTGGGTGGTGAAGATTTTGATAATAGGATGGTTAACCACTTTGTACAGGAATTTAAAAGGAAGTTCAAGAAGGATATTAGTGGTAGTGCTAGGGCTTTGAGAAGATTGAGGACCGCTTGTGAAAGGGCCAAGAGGACTCTTTCATCCACTGCTCAGACAACCATAGAGATTGATTCTTTGTATGAAGGCATCGACTTTTATTCAACAATTACTAGAGCTAGGTTCGAAGAGTTGAATATGGATCTATTCAGAAAGTGTATGGAGCCTGTTGAAAAGTGTTTGAGAGATGCTAAGATGGATAAAAGCTCTATCCATGATGCAATCGAGCAGGCAATCCAATGGTTGGATAACAACCAGCTGGCGGAGGCTGATGAATTTGATGATAAAATGAAGGAACTTGAGAGCATCTGCAACCCGATAATTGCAAAGATGTATCAAGGTGCAGGTGGTGACGTGCCTGGAGGTGGCTTTGGTGGCGTTCCTCCCGAGGGTGGACCGTCCAGTGGTGGAGGTGCTGGCCCTAAGATTGAGGAAGTGGACTAA___TCTGGCTCTCAAATGCATATGATATTACTGTATTTTCTGTCGTAAGAATTATATTATTATAAGCATAATTTTGATGTTGAAATTTATATTTAATGATGCAATTGCCGGCTATTATGACGGCGATTTTATGTTAATCTTGAACCTCAATGCAAGAGATGGCCATTGCGGTGATTTCATACGTACTTATGGTGTTGGAATATTTATTTGATGTCGCAATCAAGGGCTTTATGATGAATTTTT

Fln Alignment:

sp_v1.1_unigene59318___KGEGPAIGIDLGTTYSCVGVWQHDRVEIIANDQGNRTTPSYVGFTDTERLIGDAAKNQVAMNPTNTVFDAKRLIGRRFSDATVQSDIKMWPFKLTPGPADKPMIVVNYKGEEKQFAAEEISSMVLMKMREIAEAYLGVTIKNAVVTVPAYFNDSQRQATKDAGVIAGLNVMRIINEPTAAAIAYGLDKKAGSVGEKNVLIFDLGGGTFDVSLLTIEEGIFEVKSTAGDTHLGGEDFDNRMVNHFVQEFKRKFKKDISGSARALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYSTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSSIHDAI
B8LRY5_______________KGEGPAIGIDLGTTYSCVGVWQHDRVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPTNTVFDAKRLIGRRFSDPTVQNDMKLWPFKVISGPAEKPMIVVSYKGEDKQFAAEEISSMVLTKMKEIAEAYLGTTIKNAVVTVPAYFNDSQRQATKDAGVISGLNVMRIINEPTAAAIAYGLDKKATSVGEKNVLIFDLGGGTFDVSLLTIEEGIFEVKATAGDTHLGGEDFDNRLVNHFTQEFKRKYKKDLSSSARALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYSTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSTIHDVV

SNPs (tot: 19)

Position	A %	C %	G %	T %	Probability
1371	6		93		1.0
1519		93	1		1.0
1523		97	2		0.921055
1527			3	96	0.999856
1542	94	1	3		0.992019
1543	97		2		0.910981
1544	96		3		0.999836
1545		2		97	0.907451
1546	94		4		0.999999
1551	95	2		1	0.988836
1555	96		3		0.998449
1559	95	1	3	1	0.98972
1564		2	97		0.914425
1568		97	2		0.917783
1569			3	96	0.999856
1612		50	1		1.0
1613		50	1	47	1.0
1614	1	49	47	1	1.0
1616	1		49	17	1.0

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain