SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene59040

Length: 1674 nt

Origin of reads: Biogeco_1 Biogeco_2 Uagpf Uma

UniGene Fasta

Ace file of the UniGene sp_v1.1_unigene59040

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	24-sterol C-methyltransferase n=1 Tax=Gossypium hirsutum RepID=Q2QDF7_GOSHI	-	-	77%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	94%
Blast2go	24-sterol c-methyltransferase	-	-	88%

Source	ECs	Term	Type	e value	Identity
Blast2go	Sterol 24-C-methyltransferase.	EC:2.1.1.41	-	0.0	88%

Source	KEGGs	Term	Identity
Blast2go	Steroid biosynthesis	00100	88%
Blast2go	Biosynthesis of terpenoids and steroids	01062	88%
Blast2go	Biosynthesis of plant hormones	01070	88%
Blast2go	Metabolic pathways	01100	88%
Blast2go	Biosynthesis of secondary metabolites	01110	88%

Source	GOs	Term	Type	Identity
Blast2go	embryo development ending in seed dormancy	GO:0009793	Biological Process	88%
Blast2go	sterol biosynthetic process	GO:0016126	Biological Process	88%
Blast2go	sterol 24-C-methyltransferase activity	GO:0003838	Molecular Function	88%

Source	InterPros	Term	Type	e value	Identity
Blast2go	Methyltransferase type 11	IPR013216	-	0.0	88%
Blast2go	Sterol methyltransferase C-terminal	IPR013705	-	0.0	88%

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: C0PRC7

Fln msg:

Fln protein: MATGGGALDLASGMGGNIEKEQMLTAVEEYEKYHMYYGGDEGSRKSNYTDMVNKYYDLATSFYEYGWGESFHFAHRWKGETLRESIKRHEHFLALQLCLKPAMKVLDVGCGIGGPLREIARFSRTSITGLNNNAYQISRGKELNRQCGLDKSCDFVKGDFMKMPFSNDTFDAVYAIEATCHAPDAVGCYKEIKRVLKPGQLFAAYEWCMTDSFDPKNEEHQKIKAEIELGDGLPDIRTTNQCIEALKSAGFEVLFEENLTVSSTVPWYDPLDTSHFSLNSFRLTAIGRFLTRNMVRMLEFLRLAPEGSERVSSFLEKAAEGLVAGGRKDIFTPMYFFLARKPLNDNQS*

Protein Length: 350

Fln nts: CGTCGTGACCTTGGCTGTCACTCATTTTTCAGAAGAAACAAACCATATTTGTAGACGAACCTCAATTGTTATTATATCGTCGTGACCTTGGTTTTCTGTTTATGGGTCGGTTCATTTCCACCAGCTATGCTTGCGTTGCCACAAACCGATGTAATTTATTATTGTGCGCAGGGATTCCTTCTGCTCCTTATGATCCCCTAACCCCTAAATCGTAGCAGTGAAGCCATTAACGAATTTTGCGGGTTCAGAAAGATTCACTGAATCGCTTATTAAAACTCTGTTTCAGGA_-_ATGGCAACAGGAGGAGGAGCGTTGGATCTGGCCTCAGGAATGGGAGGCAACATTGAGAAAGAACAAATGCTGACCGCTGTTGAAGAGTACGAAAAATATCACATGTACTATGGTGGTGACGAAGGCTCGAGAAAATCTAACTATACAGACATGGTAAATAAATACTATGATCTGGCGACTAGTTTCTATGAGTATGGATGGGGGGAATCTTTTCATTTTGCTCACAGATGGAAAGGGGAGACCCTCCGAGAAAGTATAAAGCGCCATGAACATTTTCTTGCTCTTCAGCTTTGTTTAAAGCCTGCAATGAAGGTATTGGACGTTGGATGTGGGATTGGAGGTCCACTGAGAGAAATTGCTAGGTTCAGTCGGACTTCGATCACAGGATTGAATAATAATGCATATCAGATATCAAGAGGAAAAGAGCTTAACAGGCAATGTGGGCTAGACAAAAGTTGTGATTTTGTCAAGGGAGACTTCATGAAGATGCCTTTCTCCAACGATACTTTTGATGCAGTTTATGCAATTGAGGCTACCTGTCATGCACCAGATGCAGTTGGCTGCTACAAAGAGATAAAAAGAGTACTGAAACCTGGCCAACTGTTTGCGGCATATGAATGGTGCATGACTGACTCTTTTGACCCAAAGAATGAAGAACACCAGAAGATTAAGGCAGAAATTGAACTTGGAGATGGGCTTCCTGACATTCGTACTACAAACCAGTGTATTGAAGCTCTTAAATCTGCTGGATTTGAGGTGCTCTTTGAAGAGAACCTTACAGTCTCATCAACAGTTCCGTGGTATGATCCACTTGATACAAGTCATTTTTCTCTGAATAGTTTTCGTCTCACAGCCATTGGTCGCTTTTTGACAAGAAATATGGTTAGAATGTTGGAATTCCTGAGGTTAGCACCAGAAGGCAGTGAAAGGGTATCCTCATTTTTGGAGAAAGCTGCAGAAGGGCTCGTTGCAGGGGGGAGGAAAGATATTTTTACACCTATGTACTTTTTTCTGGCCCGAAAACCTTTGAACGATAACCAGTCGTAA___GGTTGACCGCATTTTTCACGAGAACAGAGACTACACCGCTTTTGTTTACAGACAGCTGAACAATTAGTTTCTTGTTTTTAAGGCATTTATTTTTTCTTGCTTCATTGTTTGGCTTTTCAACGGGGAAAGGAAATATAAACAGAAGATTGTAAAAATCATATTCCAGACGATTCAGTAGTAGAAATCAGTATGAGGGAGGAGAAAGAAGCTTGGATGGTTCTTGGTTTGTTAGAGTTGAAGATATTGCCAGGACATGAATTGTCCATGGATTCATTTGTATATGGAATCTCCTTTGTTACTTGGGTTTTTATCAATCTAAATTACTGTTTCCAGTAACCT

Fln Alignment:

sp_v1.1_unigene59040___MATGGGALDLASGMGGNIEKEQMLTAVEEYEKYHMYYGGDEGSRKSNYTDMVNKYYDLATSFYEYGWGESFHFAHRWKGETLRESIKRHEHFLALQLCLKPAMKVLDVGCGIGGPLREIARFSRTSITGLNNNAYQISRGKELNRQCGLDKSCDFVKGDFMKMPFSNDTFDAVYAIEATCHAPDAVGCYKEIKRVLKPGQLFAAYEWCMTDSFDPKNEEHQKIKAEIELGDGLPDIRTTNQCIEALKSAGFEVLFEENLTVSSTVPWYDPLDTSHFSLNSFRLTAIGRFLTRNMVRMLEFLRLAPEGSERVSSFLEKAAEGLVAGGRKDIFTPMYFFLARKPLNDNQS
C0PRC7_______________MATVGGAIDLASGVGGNIEKGQMLTAVEEYEKYHMYYGGDEDSRKSNYTDMVNKYYDLATSFYEYGWGESFHFAHRWKGESLRESIKRHEHFLALHLCLKPAMKVLDVGCGIGGPLREIARFSRTSITGLNNNAYQISRGKELNRQCGLDRSCDFVKGDFMKMPFPDNTFDAVYAIEATCHAPDAVGCYKEIKRVLKPGQLFAAYEWCMTDSFDPKNEEHQKIKAEIELGDGLPDIRTTNQCFEALKSAGFEVLFEENLTVSSTVPWYAPLDTSHFSLSSFRLTALGRLLTRNMVRILEFLRLAPEGSERVSSFLEKAAEGLVEGGRKEIFTPMYFFVARKPLNDNQS

SNPs (tot: 13)

Position	A %	C %	G %	T %	Probability
386	80		20		0.995493
387	80		20		0.995493
388	80	20			0.995493
390	80		20		0.995493
529	25		75		1.0
586		22		77	0.999999
977	85		14		0.993533
1221			12	87	0.992554
1270	14		85		0.993533
1271			85	14	0.993533
1349	11			88	0.991528
1353		88	11		0.991528
1354		88	11		0.991528

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain