SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene58646

Length: 2265 nt

Origin of reads: Biogeco_1 Uagpf Uma

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>sp_v1.1_unigene58646
CGTCGTGACCTTGGCTGTCACTCAAAGACCTGGAGGTGAGGGGTGAAGGTTCACTTGGTTGAATTGGCCAGGGCTTTGGCCAATGCTAATGTGGGTTTTTCCCGGATTTAGGGCTTTGGCCAATGCTAATGAAATCTTATTAGCTCTGAAATCCGACACCATTGTGAAGTAAATCCAATAGCAACAGCAGGCCATGGAATCAGAAACGAGAACTCCACAGTTCACTGTTGGTCAGGAGATTGCAACTTTTCTTGTGTCATGTGGACTACTGCTCGAGGTTTGGGAAGAGTCTCCTCATGCAAACAATTCAGAGGGCTTTACTCTGAATGAGCATGAAGATTTTGTATGTATTGCTTTCGCTTCGTTTCAAAGAGTAGAAGATTTCATTCTCAACCAGAGTAAATGTGGAGAGGACAATATACAGACTGTTCAACATGTCTTCTCTGAATGCCTCAAGGGTAATGATGGTGAACCAGCCTTCATTCATCCAGGAGCTCTTATGCTGTTTCTCCATATAATCGAGAAAACAGGTTTCCAAGCAAAGTTGCAGATTTACACGGACTCCCAACAAAAGAAACTCAAGCCCATCATATTTGTGGGTCAGTCCATAGGAGGGGCAGTTGCAACCTTGGCCACATTATGGGTTCTGGGAAAGAGGCTAAGGCAAAGCTCTCCCTTTTGTATCACGTTTGGTTGCCCTCTGGTCGGAGATGAAAGGCTTGTTGAAGCAGTTGGCCGTGAGAACTGGGGAGGAAACTTTTGTCACGTGGTTTCCAAGCATGATATTGTTCCTAGGATGCTCCTTGCACCGATTGAGTCAATTGCTAAGCCCTTTACTACTATTTGTTCTTATTGGCAGGGCATAAATGTGCCTGATTCTTTAATTCAAGATGCTGCCAGGACTCTTCTGAATCAGATCTTTGTTTCTCCAAGCAGCCCATACAGACCTTTTGGCAGTTACATGCTTTGCTCAAGCAATGGTGCAGCTTGTATTCAAAACGCTCAGACGGTTTTGGAAATGCTTAGTTTGACTATGCCAAGCCAACAAACTTCTTTTGATGAGATTGTAAAGGCCTGTCTTTCAGAGCACATAAGGTATGATTCTGTTTTGGAGGAGGTTTTGCAAAACTCCATAAGAGGGATACGATCAATAGAGAATTCCAATTCAGAATCCCCTTACGAGATGGGTATTTTATTGCAGCTTGAAGCTATTGGCGTGGGAGCTCAGAATGATCGTGCACAGCGTGCCCTACGAAAAGCCGGAGAAATTGAAAACAGTTTCCTCATAAATGTTGATAAGCTAGCTACTAAATTAAGCCTAAAGCAAAGTCTTATGGCAGAGATTGAATGGTATAAAGAGGGTTGTGAGAAGAATGGTGGCATCGGATATTATGATACCTTCAAGAACCATGCCAACACTCAGGATATCCATGTTGATAGGCATAGGATAAATCTCGGGGCATTTTGGGATGACATTATTGAAGCATGGGAGGGTAATGAATTGCCAAGTGATTTCCAATCCCAAAATAAGTGGATAAATGCTGGCACTGCGTATAGGAGACTTGTAGAGCCTCTGGATATTGCACATTATTATCGCATGTCTAAGGGTAATGGCAACTATCTTTCAGATGGAAGATCCAATCGCTATAAAGTTCTTCAGGAATGGATGGAAGCGAAAGAAAAAACTCGCAGTTCAAGAGGTCAGAGAACGCGCACCAAGCTTGCGTNTCNCACTGAGGATTCNNGTCNTTTGGGCACGTGTAGAGGAGGCTTGCAAAGACCTGGAAAATCTGAAACGAGGCCAACACCAAAGCCTGCAAAGTCTGGAACAGTTTGAAAAGTATGTGACAACCATGAACAGCGCTCTCAAAATTTCTCCAGATGTTTTCTTCAAAGGCAACAGCTTCATGACATGGTGGGAAGAATGGGAAAAATACAAAAGAGATCATTCCCCTTAATGGAGCTCTCCTCTCGTTTATACCAGATAATAGAAAGGGAAAGTTGGAAGAGGCGAATTTGCAGAGCATCTTATAGAAACCCTGTTTCCTCTGTTTTTGGAATTTCAGTTTCGTACATTAGATGTAATTTCTGTCTATCAGATCANATTGTNTGTNTNGTTTGCGACATGTCATTTCGTTTTATTGGTTTAAAAAATAGTTTCTCTGAAATGGTAAAATGGTTCTTCAAGGTGACTTATGATTGCTGTAATGTTATAATGGTTCTTCAGGCGGGGCTTATTGAAAATTGTCATTTTCAGTTGTACAG

Ace file of the UniGene sp_v1.1_unigene58646 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	lipase, putative	-	-	33%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	76%
Blast2go	phytoalexin-deficient 4-2 protein	-	-	65%

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: B8LRI6

Fln msg: Separated hits, possible frame ERROR between 1747 and 1752,

Fln protein: MESETRTPQFTVGQEIATFLVSCGLLLEVWEESPHANNSEGFTLNEHEDFVCIAFASFQRVEDFILNQSKCGEDNIQTVQHVFSECLKGNDGEPAFIHPGALMLFLHIIEKTGFQAKLQIYTDSQQKKLKPIIFVGQSIGGAVATLATLWVLGKRLRQSSPFCITFGCPLVGDERLVEAVGRENWGGNFCHVVSKHDIVPRMLLAPIESIAKPFTTICSYWQGINVPDSLIQDAARTLLNQIFVSPSSPYRPFGSYMLCSSNGAACIQNAQTVLEMLSLTMPSQQTSFDEIVKACLSEHIRYDSVLEEVLQNSIRGIRSIENSNSESPYEMGILLQLEAIGVGAQNDRAQRALRKAGEIENSFLINVDKLATKLSLKQSLMAEIEWYKEGCEKNGGIGYYDTFKNHANTQDIHVDRHRINLGAFWDDIIEAWEGNELPSDFQSQNKWINAGTAYRRLVEPLDIAHYYRMSKGNGNYLSDGRSNRYKVLQEWMEAKEKTRSSRGQRTRTKLASLTEDSxxxWARVEEACKDLENLKRGQHQSLQSLEQFEKYVTTMNSALKISPDVFFKGNSFMTWWEEWEKYKRDHSP*

Protein Length: 590

Fln nts: CGTCGTGACCTTGGCTGTCACTCAAAGACCTGGAGGTGAGGGGTGAAGGTTCACTTGGTTGAATTGGCCAGGGCTTTGGCCAATGCTAATGTGGGTTTTTCCCGGATTTAGGGCTTTGGCCAATGCTAATGAAATCTTATTAGCTCTGAAATCCGACACCATTGTGAAGTAAATCCAATAGCAACAGCAGGCC_-_ATGGAATCAGAAACGAGAACTCCACAGTTCACTGTTGGTCAGGAGATTGCAACTTTTCTTGTGTCATGTGGACTACTGCTCGAGGTTTGGGAAGAGTCTCCTCATGCAAACAATTCAGAGGGCTTTACTCTGAATGAGCATGAAGATTTTGTATGTATTGCTTTCGCTTCGTTTCAAAGAGTAGAAGATTTCATTCTCAACCAGAGTAAATGTGGAGAGGACAATATACAGACTGTTCAACATGTCTTCTCTGAATGCCTCAAGGGTAATGATGGTGAACCAGCCTTCATTCATCCAGGAGCTCTTATGCTGTTTCTCCATATAATCGAGAAAACAGGTTTCCAAGCAAAGTTGCAGATTTACACGGACTCCCAACAAAAGAAACTCAAGCCCATCATATTTGTGGGTCAGTCCATAGGAGGGGCAGTTGCAACCTTGGCCACATTATGGGTTCTGGGAAAGAGGCTAAGGCAAAGCTCTCCCTTTTGTATCACGTTTGGTTGCCCTCTGGTCGGAGATGAAAGGCTTGTTGAAGCAGTTGGCCGTGAGAACTGGGGAGGAAACTTTTGTCACGTGGTTTCCAAGCATGATATTGTTCCTAGGATGCTCCTTGCACCGATTGAGTCAATTGCTAAGCCCTTTACTACTATTTGTTCTTATTGGCAGGGCATAAATGTGCCTGATTCTTTAATTCAAGATGCTGCCAGGACTCTTCTGAATCAGATCTTTGTTTCTCCAAGCAGCCCATACAGACCTTTTGGCAGTTACATGCTTTGCTCAAGCAATGGTGCAGCTTGTATTCAAAACGCTCAGACGGTTTTGGAAATGCTTAGTTTGACTATGCCAAGCCAACAAACTTCTTTTGATGAGATTGTAAAGGCCTGTCTTTCAGAGCACATAAGGTATGATTCTGTTTTGGAGGAGGTTTTGCAAAACTCCATAAGAGGGATACGATCAATAGAGAATTCCAATTCAGAATCCCCTTACGAGATGGGTATTTTATTGCAGCTTGAAGCTATTGGCGTGGGAGCTCAGAATGATCGTGCACAGCGTGCCCTACGAAAAGCCGGAGAAATTGAAAACAGTTTCCTCATAAATGTTGATAAGCTAGCTACTAAATTAAGCCTAAAGCAAAGTCTTATGGCAGAGATTGAATGGTATAAAGAGGGTTGTGAGAAGAATGGTGGCATCGGATATTATGATACCTTCAAGAACCATGCCAACACTCAGGATATCCATGTTGATAGGCATAGGATAAATCTCGGGGCATTTTGGGATGACATTATTGAAGCATGGGAGGGTAATGAATTGCCAAGTGATTTCCAATCCCAAAATAAGTGGATAAATGCTGGCACTGCGTATAGGAGACTTGTAGAGCCTCTGGATATTGCACATTATTATCGCATGTCTAAGGGTAATGGCAACTATCTTTCAGATGGAAGATCCAATCGCTATAAAGTTCTTCAGGAATGGATGGAAGCGAAAGAAAAAACTCGCAGTTCAAGAGGTCAGAGAACGCGCACCAAGCTTGCGTcTCtCACTGAGGATTCgtGxxxxxxTTGGGCACGTGTAGAGGAGGCTTGCAAAGACCTGGAAAATCTGAAACGAGGCCAACACCAAAGCCTGCAAAGTCTGGAACAGTTTGAAAAGTATGTGACAACCATGAACAGCGCTCTCAAAATTTCTCCAGATGTTTTCTTCAAAGGCAACAGCTTCATGACATGGTGGGAAGAATGGGAAAAATACAAAAGAGATCATTCCCCTTAA___TGGAGCTCTCCTCTCGTTTATACCAGATAATAGAAAGGGAAAGTTGGAAGAGGCGAATTTGCAGAGCATCTTATAGAAACCCTGTTTCCTCTGTTTTTGGAATTTCAGTTTCGTACATTAGATGTAATTTCTGTCTATCAGATCAaATTGTtTGTcTaGTTTGCGACATGTCATTTCGTTTTATTGGTTTAAAAAATAGTTTCTCTGAAATGGTAAAATGGTTCTTCAAGGTGACTTATGATTGCTGTAATGTTATAATGGTTCTTCAGGCGGGGCTTATTGAAAATTGTCATTTTCAGTTGTACAG

Fln Alignment:

sp_v1.1_unigene58646___MESETRTPQFTVGQEIATFLVSCGLLLEVWEESPHANNSEGFTLNEHEDFVCIAFASFQRVEDFILNQSKCGEDNIQTVQHVFSECLKGNDGEPAFIHPGALMLFLHIIEKTGFQAKLQIYTDSQQKKLKPIIFVGQSIGGAVATLATLWVLGKRLRQSSPFCITFGCPLVGDERLVEAVGRENWGGNFCHVVSKHDIVPRMLLAPIESIAKPFTTICSYWQGINVPDSLIQDAARTLLNQIFVSPSSPYRPFGSYMLCSSNGAACIQNAQTVLEMLSLTMPSQQTSFDEIVKACLSEHIRYDSVLEEVLQNSIRGIRSIENSNSESPYEMGILLQLEAIGVGAQNDRAQRALRKAGEIENSFLINVDKLATKLSLKQSLMAEIEWYKEGCEKNGGIGYYDTFKNHANTQDIHVDRHRINLGAFWDDIIEAWEGNELPSDFQSQNKWINAGTAYRRLVEPLDIAHYYRMSKGNGNYLSDGRSNRYKVLQEWMEAKEKTRSSRGQRTRTKLASLTEDSCxxWARVEEACKDLENLKRGQHQSLQSLEQFEKYVTTMNSALKISPDVFFKGNSFMTWWEEWEKYKRDH
B8LRI6_______________MESDSLTSRFTIGQEIATFLVSCGLVPKAWEESLRASNVESFTVNEYEDVAYVTFPSFQRLEDFIVDDSKCGEGNIQTDHGFFSGCLNGNDEKPALIHPGALTLFLHIMEKTDFQAKLQIYTDAKQRKLKPIIFVGHSLGGAVATLATLWVLGKRLRQSSPFCITFGCPLVGDERLVEAVGRENWGGNFCHVVSKHDIVPRMLLAPFESIANPFTTVFGYWQGKNVPDSLIQDASRTLLNHVLVSPSSPYKPFGTYMFCSSNGAACIENAQTVLEMLHLTMQSQHTSFDEIVQACLLEHIRYDSVLEEVRQNSIRGIR-IAKSNSESSYEMGISSQLEAIGVGAQNDRAQLALRKAGEIENNYNENVETLAIKLSVRQSSMAELEWYKERCEKEDGSTYYDSFKKQ-DMKDIHANLVRVKLAEFWDEIMEMWEGHELPSDFKSQNKWINAGNTYRKLVEPLDIAHYYLTTKTNKSYFSDGRPNRHKVLQEWMEAKEKTRSSRGQRTRRKPASLTEDSCxxWAYVEEAWKDLENLKQGQHQSLQSLEQFEKYVTTMNNALKIAPDVFLNGSNYMMWSEEWEKYKRDH

SNPs (tot: 109)

Position	A %	C %	G %	T %	Probability
192	25		75		0.996473
1153	25			75	0.996473
1174		75		25	0.996473
1178		20		80	0.995495
1200			50	50	1.0
1218	66		33		0.997452
1228	33		66		0.997452
1314		50	50		1.0
1411		66		33	0.997452
1434	33		66		0.997452
1443	33			66	0.997452
1467	66			33	0.997452
1478			33	66	0.997452
1502		66		33	0.997454
1503		66	33		0.997454
1514	66	33			0.997459
1518			33	66	0.997452
1520		33		66	0.997476
1521	33	66			0.997452
1523			33	66	0.997452
1524	33	66			0.997452
1527		66	33		0.997452
1589	25	75			0.996473
1675		80	20		0.995493
1712		14		85	0.993533
1715	14		85		0.993533
1748		85		14	0.993533
1759		12	87		0.992554
1789			12	87	0.992555
1828			88	11	0.991528
1829		88	11		0.991528
1836	88	11			0.991528
1837		11	88		0.991528
1841	88			11	0.991528
1844		11		88	0.991528
1845			77	22	0.999999
1846	11			88	0.991528
1847		11		88	0.991528
1863	12			87	0.992554
1866		87	12		0.992554
1867	12	87			0.992554
1868		12		87	0.992554
1869	12	87			0.992554
1870			12	87	0.992554
1874	12	87			0.992554
1875	87		12		0.992554
1877			12	87	0.992554
1879		12		87	0.992554
1882			12	87	0.992554
1883	12	87			0.992554
1884		12		87	0.992554
1891		14	85		0.993541
1930		25		75	1.0
1952		87	12		0.992554
1955			12	87	0.992554
1957		87	12		0.992554
1960		12	87		0.992554
1964		12		87	0.992554
1965	87	12			0.992554
1969		87	12		0.992554
1970		87		12	0.992554
1972	14			85	0.993533
1977	85	14			0.993533
1978			14	71	0.984337
1981		20		80	0.995493
1990	20		80		0.995493
2007		50		50	1.0
2008			33	66	1.0
2009	33	66			1.0
2011	50		16	33	1.0
2016		25		75	1.0
2025			25	75	1.0
2027	75			25	1.0
2031	12		25		1.0
2033	62	25	12		1.0
2034	12		87		0.992554
2047		83	16		0.994516
2055		85		14	0.993533
2067		12	87		0.992554
2070		12	87		0.992554
2082	16	83			0.994512
2138		83	16		0.994512
2141	83			16	0.994512
2143	16	83			0.994512
2144		16		83	0.994512
2146	83		16		0.994512
2147		16		83	0.994512
2148	83	16			0.994512
2152			16	83	0.994512
2153			14	85	0.993538
2155		83	16		0.994512
2158		14		85	0.993536
2159			33	66	1.0
2162	16	16			0.988813
2165	80	20			0.995493
2166	20			80	0.995493
2167	20			80	0.995493
2170		83		16	0.994514
2174	83	16			0.994515
2176	16	83			0.994515
2178		83	16		0.994515
2248		66	33		0.997452
2249	33	66			0.997452
2250		66	33		0.997452
2252		66	33		0.997452
2255	33	66			0.997452
2256			33	66	0.997452
2266	25			75	0.996481
2269		25		75	0.996481

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain