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Fln status: Putative C-terminus

Fln database: sp_plants

Fln subject: P93736

Fln msg: Overlapping hits, possible frame ERROR between 320 and 320, query STOP codon too far from subject stop. Distance to subject end: 62 aas, putative chimeric sequence, your sequence is shorter than subject: 385 - 1108

Test code: 0

Test Code was used to find complete genes when there was not found a reliable orthologue. The best ORF (Open Reading Frame) is shown, and only ORFs > 200pb were analyzed. Your ORF will be more reliable if a stop codon was found before the start codon. A Test Code value > 0.95 means the ORF is probably coding. A Test Code value < 0.74 means the ORF is probably non-coding. Test Code values in between 0.74 and 0.95 mean it is uncertain whether the ORF is coding or not.

Fln protein: ARMVMLGMKLTGEVPFKQVYLHPMVRDAHGRKMSKSLGNVIDPLEVINGITLEDLHKRLEQGNLDRNELDIAKAGQKQDFPNGIAECGTDALRFSLVAYTAGSDKxxLDIQRVVGYRHWCNKLWNAIRFAMLNLGTDFLPSENLDVKSLPCGCKWILSVLNKAISKTVTSMEVYDFSSATNAVYSWWQYQLCDVFIGIIKPVMSSDDNNSASASVKRVTRDTLWICLDNGLRLLHPFMPFVTEELWQRLPQRLGDTSRKESIMISDYPTVVEEWTDDEIESQMVLIESSVRSIRSLRMSYLLLPKQRPEAFLLCKTDEVATVVKKGATEICTLASLASLKVLREGDVPPVGCAVNIVNEHLSVYLLLRGIVDAQLELSKRRDLD*

Protein Length: 386

Fln nts: TAGCACGCATGGTAATGCTAGGAATGAAACTTACAGGAGAAGTTCCTTTTAAGCAGGTTTATTTGCATCCAATGGTACGTGATGCACATGGTCGTAAAATGTCCAAGTCATTAGGCAATGTGATTGATCCTTTGGAAGTCATAAATGGTATAACATTGGAGGATCTGCATAAAAGGCTGGAGCAGGGTAATTTAGATAGAAATGAACTTGACATTGCAAAAGCAGGACAAAAGCAAGACTTTCCAAATGGCATAGCAGAGTGTGGGACAGATGCCCTCCGTTTTTCTCTTGTGGCCTACACTGCTGGGTCAGATAAAxxxxxxTTGGATATCCAGCGGGTAGTTGGTTATCGACATTGGTGTAATAAACTATGGAATGCTATCCGCTTTGCTATGCTCAACTTGGGAACAGATTTTTTGCCTTCTGAGAATTTGGATGTGAAATCTCTGCCATGTGGTTGCAAGTGGATACTTTCTGTTCTAAATAAAGCTATCTCAAAGACTGTCACAAGCATGGAAGTATATGATTTCTCCAGTGCAACCAATGCAGTTTACTCGTGGTGGCAATATCAATTATGTGATGTTTTTATTGGGATAATTAAGCCAGTCATGTCGTCAGATGATAATAATTCAGCATCAGCATCAGTAAAACGCGTTACAAGGGATACATTATGGATATGCCTGGATAATGGATTGCGGTTATTGCATCCGTTCATGCCCTTTGTTACTGAAGAGTTATGGCAACGCTTACCCCAAAGACTAGGGGATACCTCGAGGAAAGAATCAATTATGATTTCTGATTATCCTACAGTCGTCGAGGAGTGGACAGATGATGAAATTGAATCACAGATGGTTCTTATTGAATCATCAGTAAGGTCAATAAGATCTCTTCGAATGTCTTATTTATTACTTCCAAAGCAAAGGCCAGAGGCTTTCCTTCTATGCAAAACAGATGAAGTGGCCACAGTAGTCAAAAAGGGTGCTACAGAGATTTGCACGCTTGCTTCCTTAGCATCACTGAAGGTACTCCGGGAGGGTGATGTGCCTCCTGTTGGCTGCGCAGTAAATATTGTGAATGAGCATCTATCTGTATACTTGTTACTCCGAGGCATTGTAGATGCACAGCTTGAACTTTCAAAACGTCGTGACCtagactGaaActcgAACTTATCCGGCAACAGGAAGGAGTGACAAAGAAAATGAACATTCCCGGATATAGAGACAAaGTACgtGCCAcTATacaaGtcGaacATCTTTCTAAGCTGAATAAACTTATGGCTGAATTGAGCATCGTCGAAGAGGCAAATGAGAATTTTGAAAAACTTTTGGCAGATAGCAGCTAAAACAATGAGTTGAATTGCAGTATTTTCTCACTATTGATTTTCAAGTATTgATAGATCATAATTTGTGCCCATTAAATTCCATTCTTGGTATGGAACTCAATATATTTATTATTTCTGCTTTCAAACGGTGTCCTCGAGTAGAAA

Fln Alignment:

sp_v1.1_unigene58631___ARMVMLGMKLTGEVPFKQVYLHPMVRDAHGRKMSKSLGNVIDPLEVINGITLEDLHKRLEQGNLDRNELDIAKAGQKQDFPNGIAECGTDALRFSLVAYTAGSDKINLDIQRVVGYRHWCNKLWNAIRFAMLNLGTDFLPSENLDVKSLPCGCKWILSVLNKAISKTVTSMEVYDFSSATNAVYSWWQYQLCDVFIGIIKPVMXXXXXXXXXXXVKRVTRDTLWICLDNGLRLLHPFMPFVTEELWQRLPQRLGDTSRKESIMISDYPTVVEEWTDDEIESQMVLIESSVRSIRSLRMSYLLLPKQRPE---AFLLCKTDEVATVVKKGATEICTLASLASLKVLREGD-VPPVGCAVNIVNEHLSVYLLLRGIVDAQLELSKRRD
P93736_______________ARMVMMGMKLGGEVPFSKVYFHPMIRDAHGRKMSKSLGNVIDPLEVINGVTLEGLHKRLEEGNLDPKEVIVAKEGQVKDFPNGIPECGTDALRFALVSYTAQSDKINLDILRVVGYRQWCNKLWNAVRFAMMKLGDGYTPPQTLSPETMPFSCQWILSVLNKAISKTVVSLDAFEFSDAANTIYAWWQYQFCDVYIEAIKPYFAGDNPTFASE--RAHAQHALWISLETGLRLLHPFMPFVTEELWQRLPAP-KDTERKASIMICDYPSAIENWSNEKVESEMDTVLATVKCMRALRAG--LLEKQKNERLPAFALCENNVTSEIVKSHELEIRTLANLSSLEVVSKGQHAAPPGSSVETVNENLKVYLEVDGAINTEAEQEKIRN