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Fln status: Internal

Fln database: tr_plants

Fln subject: A5AL34

Fln msg: Overlapping hits, possible frame ERROR between 54 and 54, and possible frame ERROR between 278 and 317, Unexpected STOP codon at 3' end. Distance to subject end: 499 aas, your sequence is shorter than subject: 153 - 1861

Test code: 0

Test Code was used to find complete genes when there was not found a reliable orthologue. The best ORF (Open Reading Frame) is shown, and only ORFs > 200pb were analyzed. Your ORF will be more reliable if a stop codon was found before the start codon. A Test Code value > 0.95 means the ORF is probably coding. A Test Code value < 0.74 means the ORF is probably non-coding. Test Code values in between 0.74 and 0.95 mean it is uncertain whether the ORF is coding or not.

Fln protein: MGTLLNFLVSCPKGTMFxxSVDASAHIKDARTLCELLDIFIQEVGSSNVVQVITDNAANYVAVGNMLMERYPNIF*TPCVAHCIDVMLEDIGxxxxxxxxxxxxxxITKFIYNHASMLHLMRMFINNKELVRSAITRVATSFISLQSLLNSM*

Protein Length: 154

Fln nts: ATGGGGACCCTTCTTAACTTCTTGGTTAGTTGTCCCAAGGGGACAATGTTTxxxxxxTCTGTTGATGCATCTGCACACATTAAAGATGCACGTACATTATGTGAGTTGTTGGACATCTTCATTCAAGAAGTAGGCTCAAGCAATGTTGTCCAAGTCATAACGGACAATGCTGCAAATTATGTTGCTGTTGGCAATATGCTTATGGAGAGGTATCCTAATATATTTTAGACACCTTGTGTTGCACATTGCATTGATGTGATGTTGGAGGACATAGGAAxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxACATTACAAAATTTATATATAATCATGCATCTATGCTTCATTTGATGAGAATGTTCATCAACAACAAGGAGTTGGTGCGCTCTGCTATTACACGGGTTGCCACCAGTTTCATATCTCTTCAATCCCTCTTGAATTCTATGTAGGATGTGAAGATAATGTTTCTTTTAGATCAGTGGCGTGC

Fln Alignment:

sp_v1.1_unigene57741___LLNFLVSCPKGTMFMESVDASAHIKDARTLCELLDIFIQEVGSSNVVQVITDNAANYVAVGNMLMERYPNIF*TPCVAHCIDVMLEDIGKxxxxxxxxxxxxxITKFIYNHASMLHLMRMFINNKELVRSAITRVATSFISLQSLLNSM*DVKIMFLLDQW
A5AL34_______________LINFLVNSPAGTWFMKSIDASBIIKNGELMFKYLDEVVEEIGEENVVQVITDNASNYVNAGMRLMEKRSRLWWTPCAAHCIDLMLEDIGKxxxxxxxxxxxxxVVKFIYGHIWVLSLMRTFTKNHELIRPAITRFATAFLTLQSLYKQKQALIAMFSSEKW