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Fln status: Putative N-terminus

Fln database: tr_plants

Fln subject: B9SXL5

Fln msg: ERROR#3, very serious frame error, Overlapping hits, possible frame ERROR between 216 and 216, Distance to subject end: 196 aas, atg_distance in limit (1-15): atg_distance = 4, Unexpected STOP codon in 5 prime region, your sequence is shorter than subject: 123 - 332

Test code: 1

Test Code was used to find complete genes when there was not found a reliable orthologue. The best ORF (Open Reading Frame) is shown, and only ORFs > 200pb were analyzed. Your ORF will be more reliable if a stop codon was found before the start codon. A Test Code value > 0.95 means the ORF is probably coding. A Test Code value < 0.74 means the ORF is probably non-coding. Test Code values in between 0.74 and 0.95 mean it is uncertain whether the ORF is coding or not.

Fln protein: MELTEDVMKSMEIGAVFKDYNGKINSLDFHRTNNYLVTASDDEAIRLFDTASATWQKTSYSxxYGVDLICFTNHPTSVLYSSKNGWDESLRHLSLMDNKYLRYFKGHHDRVVPFVCHQRVSAS

Protein Length: 124

Fln nts: TGAAGGGAGGAGAAATTAGATAAATTATCG_-_ATGGAACTCACAGAAGACGTCATGAAAAGCATGGAGATTGGTGCTGTCTTTAAAGATTATAATGGGAAAATCAATTCGTTGGATTTTCACAGAACGAACAATTATTTGGTTACGGCTAGTGATGATGAGGCTATTCGGCTTTTCGACACTGCAAGTGCAACATGGCAAAAGACCAGTTATAGTxxxxxxTATGGTGTTGACTTGATTTGCTTCACCAACCATCCAACATCAGTACTGTACTCTTCGAAAAATGGCTGGGATGAATCATTGCGGCATCTTTCCCTCATGGACAATAAATATCTGCGTTATTTCAAAGGTCACCATGATAGGGTGGTTCCCTTTGTATGTCACCAAAGGGTGAGTGCTTCAT

Fln Alignment:

sp_v1.1_unigene54096___EKLDKLSMELTEDVMKSMEIGAVFKDYNGKINSLDFHRTNNYLVTASDDEAIRLFDTASATWQKTSYSKKYGVDLICFTNHPTSVLYSSKNGWDESLRHLSLMDNKYLRYFKGHHDRVV
B9SXL5_______________EREEKVSLELTEEILQSMEIGMVFRDYNGRISSMDFHKTSSYLVTASDDESIRLYDVSSAACLKTINSKKYGVDLVCFTSHPTTVIYSSKNGWDESLRLLSLNDNKYLRYFKGHHDRVV