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Fln status: Putative N-terminus

Fln database: coniferopsida.fasta

Fln subject: D5AAE3

Fln msg: Separated hits, possible frame ERROR between 70 and 288, Distance to subject end: 27 aas, atg_distance in limit (1-15): atg_distance = 5, No stop codon before M and low homology subject, your sequence is shorter than subject: 112 - 224

Test code: 0

Test Code was used to find complete genes when there was not found a reliable orthologue. The best ORF (Open Reading Frame) is shown, and only ORFs > 200pb were analyzed. Your ORF will be more reliable if a stop codon was found before the start codon. A Test Code value > 0.95 means the ORF is probably coding. A Test Code value < 0.74 means the ORF is probably non-coding. Test Code values in between 0.74 and 0.95 mean it is uncertain whether the ORF is coding or not.

Fln protein: MQTFRGMVDFGCKxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxYNSLLRSLCKEGRLQEAYRIFDSMK

Protein Length: 113

Fln nts: TGCAAGAGCCCAGCTACCGCAAGATGCC_-_ATGCAGACCTTTAGAGGAATGGTGGATTTTGGTTGCAAACCxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxTTACAATTCTCTCTTGCGTTCTCTTTGTAAGGAAGGACGATTACAAGAGGCTTACAGGATATTTGATTCAATGAAAGT

Fln Alignment:

sp_v1.1_unigene50768___QDAMQTFRGMVDFGCKPxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxYNSLLRSLCKEGRLQEAYRIFD
D5AAE3_______________REARNLLDGMLESGCKPxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxYAAFLKALCRAGKVAEAYEVFD