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Fln status: Internal

Fln database: coniferopsida.fasta

Fln subject: A9NW30

Fln msg: Overlapping hits, possible frame ERROR between 167 and 167, Unexpected STOP codon at 3' end. Distance to subject end: 139 aas, your sequence is shorter than subject: 80 - 247

Test code: 0

Test Code was used to find complete genes when there was not found a reliable orthologue. The best ORF (Open Reading Frame) is shown, and only ORFs > 200pb were analyzed. Your ORF will be more reliable if a stop codon was found before the start codon. A Test Code value > 0.95 means the ORF is probably coding. A Test Code value < 0.74 means the ORF is probably non-coding. Test Code values in between 0.74 and 0.95 mean it is uncertain whether the ORF is coding or not.

Fln protein: LNPPFVFSTPIAHFPRLTNVSDQHALLAFKSAITYDPYQSLAATWKPSVSFCQWxxIICSRRRQRVISLDVSSMGLQDS*

Protein Length: 81

Fln nts: TTCTCAATCCTCCTTTTGTCTTTTCCACACCCATCGCCCATTTCCCTCGTCTCACCAATGTTTCTGATCAACACGCTCTGCTTGCTTTCAAGTCTGCCATCACATATGATCCCTATCAATCATTAGCTGCCACCTGGAAACCAAGTGTTTCATTCTGCCAGTGGxxxxxxATCATATGCAGCAGGCGCAGGCAGCGAGTCATTTCCCTCGATGTTTCAAGCATGGGATTACAAGATAGTTGATGGACTACTTTCAGGAAGGTAC

Fln Alignment:

sp_v1.1_unigene48556___PFVFST-PIAH----FPRLTNVSDQHALLAFKSAITYDPYQSLAATWKPSVSFCQWTGIICSRRRQRVISLDVSSMGLQD
A9NW30_______________PSIFSTLQLSHRRHPHPRLGNASDQEPLLGFLSAITYDPSQNLTTTWTPNVSFCKWTGVICSRHRQRVVSLDVSYMGLEE