SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene44560

Length: 1321 nt

Origin of reads: Biogeco_1 Biogeco_2 Uagpf Uma

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v1.1_unigene44560 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Whole genome shotgun sequence of line PN40024, scaffold_7.assembly12x (Fragment) n=1 Tax=Vitis vinifera RepID=D7THM2_VITVI	-	-	68%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	89%
Blast2go	catalytic hydrolase	-	-	78%

Source	InterPros	Term	Type	Identity
Blast2go	Haloacid dehalogenase-like hydrolase	IPR005834	-	78%
Blast2go	HAD-superfamily hydrolase, subfamily IA, variant 3	IPR006402	-	78%
Blast2go	Pyrimidine 5-nucleotidase	IPR010237	-	78%
Blast2go	HAD-like domain	IPR023214	-	78%

Full-Lengther Next Prediction

Fln status: Putative Complete

Fln database: coniferopsida.fasta

Fln subject: A9NL51

Fln msg: query STOP codon is far from subject stop. Distance to subject end: 28 aas, your sequence is shorter than subject: 250 - 276

Fln protein: MVLDENRRVTDQGPQYDCLLFDLDDTLYPLSSGLAAACTNNIRDYVNQKLGVEKSKVADLSRELYQIYGTTMAGLRAMGYEFDYDDYHSFVHGRLPYENLKPDPVLKNLLLSMPQRKIIFTNSDKVHAAKVLNRLGLDDCFEGIICFETLNPPSEITESSNELDMPTDNSTIRKTPIICKPSKEAMEQALRLANADPQRTIFFDDSHRNIAAGKRAGLHTVLVGTSVRTEGADFAIKSIRNIREALPQI*

Protein Length: 251

Fln nts: CGTCGTGACCTTGGCTGTCACTCACCCCTCCCATTCCATTTCAATCTCGTTAGCTTGTTTTTCCCGTTCTGTCGTCGTGACCTTGGAGTCCCCAATTTCTTTCAACATACATTGCCCCCTTACCTATTACATTCACCCAGTATCTGCTTCTTCTTCCCATTTCTTTCAACCTACATTGCCCCTTCCCTATTACGTTCACCCAGAGTCATCCATA_-_ATGGTGCTGGATGAGAATAGGCGCGTGACAGACCAGGGGCCTCAATATGACTGCCTTCTGTTTGATCTGGATGACACATTATATCCCTTAAGTTCAGGACTCGCTGCAGCATGCACCAACAACATCAGAGATTATGTGAATCAAAAACTGGGAGTTGAAAAGAGCAAAGTCGCAGACTTAAGCAGAGAGCTGTATCAGATTTATGGGACAACCATGGCTGGACTTAGGGCAATGGGCTATGAATTCGACTATGACGATTACCACAGTTTTGTTCATGGAAGATTGCCATATGAGAATCTTAAACCAGATCCTGTGCTGAAGAACTTGTTATTAAGCATGCCTCAGAGGAAAATTATATTTACAAACAGTGACAAAGTTCATGCTGCAAAGGTCCTTAACAGGCTGGGGTTGGACGATTGCTTTGAAGGCATAATATGCTTTGAGACACTTAATCCTCCTTCAGAAATTACTGAAAGCAGTAATGAATTGGATATGCCTACTGATAACTCTACAATCCGGAAGACACCCATAATTTGTAAGCCCTCGAAAGAGGCCATGGAGCAGGCTCTTCGACTTGCAAACGCTGATCCACAAAGAACGATTTTCTTTGATGACAGTCACCGGAATATAGCCGCAGGAAAGCGTGCAGGCCTGCATACCGTATTGGTGGGAACTTCGGTCCGAACAGAGGGAGCAGATTTTGCCATTAAAAGTATTCGCAACATTAGAGAAGCCCTGCCTCAGATATAG___AGCACAGGCCATTATCCAGCGAATACAGGCTTGCCATGAATCTTTTTGTCATGATGTCTGATTTGCCATTTCCTCTGCAATGCGCAGCTATTTTGCCTGGAATATCTTTTTAGCATCGTTTTTTTCTTTTGTCATTATTATGCTGGAATCTACTCTTGAATTTTAGATCTGTGCTTTCGGTCGATCTGTCCTGCACCTTTCTATGGGTCTTATGTTTTGTAATCTATATGTGGATCTTCTTTTATTGTTGGGTCTGTTTTGAGACAATTTTGGGTCTTGGGGGCTTTTTGGAGGTAATGTACAGGCTTTCTTTTATTTGTTCCAGATTGGAGTCTTTAGATCATAGGTACATGAGTC

Fln Alignment:

sp_v1.1_unigene44560___MVLDENRRVTDQGPQYDCLLFDLDDTLYPLSSGLAAACTNNIRDYVNQKLGVEKSKVADLSRELYQIYGTTMAGLRAMGYEFDYDDYHSFVHGRLPYENLKPDPVLKNLLLSMPQRKIIFTNSDKVHAAKVLNRLGLDDCFEGIICFETLNPPSEITESSNELDMPTDNSTIRKTPIICKPSKEAMEQALRLANADPQRTIFFDDSHRNIAAGKRAGLHTVLVGTSVRTEGADFAIKSIRNIREALPQI
A9NL51_______________MALDENRRVTDQRPRYDCLLFDLDDTLYPLSSGLAAACTNNIGDYVNQKLGVEKSRVPDLSRELYKTYGTTMAGLRAIGYEFDYDDYHSFVHGRLPYENLKPDPVLKNLLLSMPQRKIIFTNSDKVHAAKVLNRLGLEDCFEGIICFETLNPPN-ITESNNEWGMPTVNSTVPKTPIICKPAKEAMEQALRLANADPQRTIFFDDSPRNIAAGKCAGLHTVLVGTSVRTEGADFALESIHNIREALPEI

SNPs (tot: 25)

Position	A %	C %	G %	T %	Probability
74	66		33		0.997476
369		20	80		0.995493
392		80		20	0.995497
403		33		66	0.997452
406			33	66	0.997452
429		50	50		1.0
456		16	83		0.994512
457	16		83		0.994512
1074		15		84	1.0
1138	92			8	0.999718
1139		92		8	0.999718
1226		10		89	0.999919
1229		10		89	0.999919
1239		5	11	82	0.999919
1243			71	28	1.0
1247	30			7	1.0
1248		7	30	61	1.0
1251	66		33		1.0
1253	20		80		0.995499
1254	20		80		0.995499
1255	20			80	0.995499
1256	20	80			0.995499
1258		80		20	0.995505
1259	20		80		0.995505
1261	20	80			0.995505

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain