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Fln status: Putative N-terminus

Fln database: coniferopsida.fasta

Fln subject: C0PPQ9

Fln msg: ERROR#3, very serious frame error, Overlapping hits, possible frame ERROR between 377 and 377, Unexpected STOP codon at 3' end. Distance to subject end: 238 aas, atg_distance in limit (1-15): atg_distance = 6, Unexpected STOP codon in 5 prime region, your sequence is shorter than subject: 93 - 408

Test code: 0

Test Code was used to find complete genes when there was not found a reliable orthologue. The best ORF (Open Reading Frame) is shown, and only ORFs > 200pb were analyzed. Your ORF will be more reliable if a stop codon was found before the start codon. A Test Code value > 0.95 means the ORF is probably coding. A Test Code value < 0.74 means the ORF is probably non-coding. Test Code values in between 0.74 and 0.95 mean it is uncertain whether the ORF is coding or not.

Fln protein: MSSLWRVINYSNTHTSQPTLGAAHRHLMKDQGSLPYEQVQNLIERCLQLYMNQAEVVNTLLNQAKxxPGFTGLVWQKLEEQNPDFLRHTIPD*

Protein Length: 94

Fln nts: ATGGGGAACAACGCATTTGGTGTGTATTTTCATTTTCTTTGCTGGAGACTCAGTGTCTGGAATCTAACGTAATGTGATATGCGGCGGCTTCTTCTCACACAGTGATCAACTGCTCGGATACACACGCAGCCCAGTCAAAACTTTGGAGCAGCAGAGCGCCATCTCATGAAACACCAAGG_-_ATGTCTTCCCTAtGgcgAGTGATCAACTACTCAAATACACACACATCCCAGCCAACCCTCGGAGCAGCTCACCGCCATCTCATGAAAGACCAAGGATCTCTTCCCTACGAACAGGTGCAGAATTTGATAGAACGATGCCTACAACTGTACATGAATCAAGCAGAAGTCGTGAACACTCTTTTAAATCAGGCCAAAxxxxxxCCTGGCTTCACTGGACTTGTATGGCAAAAGCTGGAAGAGCAAAATCCAGATTTCTTAAGGCATACTATACCAGATTAACTGTGAAACAACAGATCACAAAGTTCAATCAGCTGCTTC

Fln Alignment:

sp_v1.1_unigene35599___VQNLIERCLQLYMNQAEVVNTLLNQAKMEPGFTGLVWQKLEEQNPDFLR
C0PPQ9_______________VQNLIERCLQLYMSQDEVIKTLLNQARIEPGFTSLVWQKLEEQNHDFFK