SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene33569

Length: 1878 nt

Origin of reads: Biogeco_1 Biogeco_2 Uagpf Uma

UniGene Fasta

Ace file of the UniGene sp_v1.1_unigene33569

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	CLPC1; CLPC1; ATP binding / ATP-dependent peptidase/ ATPase; K03696 ATP-dependent Clp protease ATP-binding subunit ClpC	-	-	77%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	52%
Blast2go	atp-dependent protease	-	-	89%

Source	GOs	Term	Type	Identity
Blast2go	chloroplast organization	GO:0009658	Biological Process	89%
Blast2go	protein import into chloroplast stroma	GO:0045037	Biological Process	89%
Blast2go	regulation of chlorophyll biosynthetic process	GO:0010380	Biological Process	89%
Blast2go	ATP-dependent peptidase activity	GO:0004176	Molecular Function	89%
Blast2go	binding	GO:0005488	Molecular Function	89%
Blast2go	cell wall	GO:0005618	Cellular Component	89%
Blast2go	chloroplast stroma	GO:0009570	Cellular Component	89%
Blast2go	chloroplast thylakoid membrane	GO:0009535	Cellular Component	89%
Blast2go	mitochondrion	GO:0005739	Cellular Component	89%

Source	InterPros	Term	Type	Identity
Blast2go	UVR domain	IPR001943	-	89%
Blast2go	AAA+ ATPase domain	IPR003593	-	89%
Blast2go	ATPase, AAA-type, core	IPR003959	-	89%
Blast2go	Clp, N-terminal	IPR004176	-	89%
Blast2go	Chaperonin ClpA/B, conserved site	IPR018368	-	89%
Blast2go	Double Clp-N motif	IPR023150	-	89%

Full-Lengther Next Prediction

Fln status: Putative Complete

Fln database: coniferopsida.fasta

Fln subject: B8LQS4

Fln msg: STOP codon was not found. Distance to subject end: 5 aas, your sequence is longer than subject: 555 - 463

Fln protein: MAGTLAQSAILPGSIINSKYGPVKGSKRSRRAVKMIGNAHAHSFPMQTFSGLRRVTSIDNIACPRKDFHAAVAAAVYLPNSKGNARRGVVVAMFERFTEKAIKVIMLAQEEARRLGHNFVGTEQILLGLIGEGTGIAAKVLKSMGINLKEARIEVEKVIGRGSGFVAVEIPFTPRAKRVLELSLEEARQLGHSYIGSEHLLLGLLREGEGVAARVLENLGAEPSNIRTQVVRMVGENRDDDRAVVGPGSGSNKTPTLEEYGTNLTKLAEEGKLDPVVGRQPQIERVTQILGRRTKNNPCLIGEPGVGKTAIAEGLAQRIASGDVPETIEGKKVITLDMGLLVAGTKYRGEFEERLKKLMEEVKQSDDLILFIDEVHTLIGAGAAEGAIDAANILKPALARGELQCIGATTLDEYRKHIEKDPALERRFQPVQVPEPTVDETIQILKGLRERYEIHHKLRYTDEALVSAAQLSHQYISDRFLPDKAIDLIDEAGSRVRLRHAQLPEEARELEKELRQITKEKNEAVRSQDFEKAGELRDREMELKAQIVAITEKGK

Protein Length: 556

Fln nts: CGTCGTGACCTTGGCTGTCACTCGAAGTTTCAGTGTGTTGTCATTTTCTTATTAGGTTATTGTAGGGGCAGTCGGGCCGGCCATCCGGAGACATTGTTCCATTATAATTTCCCCCGGGGCAGTTTGTTGGGCATTTGATGATCCTCCTGCTCCTAGAGCAATAAGGTTCTGTATAACTTGTCAAGTGAAAATTAATCTGGGTAGGGTGTAAAG_-_ATGGCTGGGACACTTGCACAATCAGCAATTCTGCCGGGTAGCATAATCAACTCAAAATATGGTCCAGTAAAGGGATCCAAAAGATCAAGGAGAGCTGTAAAGATGATTGGAAATGCACATGCCCATTCTTTCCCCATGCAGACATTTTCTGGTTTACGACGAGTAACATCCATTGACAATATTGCATGTCCTCGGAAAGACTTTCATGCGGCAGTTGCAGCTGCCGTATATCTTCCAAATTCAAAGGGAAATGCAAGACGAGGGGTGGTTGTTGCTATGTTTGAACGTTTTACTGAAAAAGCCATTAAAGTAATTATGCTTGCTCAGGAAGAAGCAAGACGCCTTGGACACAACTTTGTAGGTACAGAGCAGATACTGTTAGGTCTTATTGGTGAGGGAACAGGCATTGCAGCCAAAGTATTGAAGTCTATGGGGATTAATCTTAAGGAGGCTCGGATTGAGGTTGAGAAAGTTATTGGTAGAGGCAGCGGATTTGTTGCTGTGGAAATTCCATTCACTCCCAGAGCAAAGCGTGTATTGGAACTGTCATTGGAGGAAGCCAGACAACTTGGCCACAGTTACATAGGATCTGAGCACCTGCTTTTGGGATTACTAAGGGAGGGTGAAGGTGTTGCAGCTCGGGTGCTTGAAAATCTTGGAGCAGAACCGAGTAACATTCGTACGCAAGTAGTTCGCATGGTGGGAGAAAATAGAGATGATGATAGAGCTGTGGTCGGACCTGGAAGCGGCAGTAACAAGACTCCAACACTTGAAGAGTATGGAACGAATTTGACTAAGTTGGCAGAGGAGGGAAAGCTTGATCCAGTGGTTGGAAGGCAGCCACAAATAGAACGGGTTACTCAAATTTTGGGACGTCGTACAAAGAATAATCCTTGTCTTATTGGTGAGCCAGGTGTGGGAAAGACAGCTATTGCTGAGGGTCTTGCCCAGCGAATTGCATCTGGGGATGTTCCAGAAACGATTGAGGGTAAAAAAGTGATTACCTTAGATATGGGCTTACTTGTTGCTGGGACCAAGTACCGGGGAGAGTTTGAAGAAAGATTAAAGAAGTTAATGGAGGAAGTTAAACAGAGTGATGATCTAATACTCTTCATAGATGAAGTGCATACCTTAATTGGAGCAGGAGCAGCAGAAGGTGCTATTGATGCTGCCAACATTCTAAAGCCTGCTCTTGCACGCGGCGAGCTACAGTGCATCGGGGCCACCACACTTGATGAATACAGAAAACACATTGAGAAGGACCCAGCTCTTGAAAGGAGATTCCAGCCAGTCCAGGTTCCAGAGCCTACTGTTGATGAGACCATACAGATTTTGAAAGGTTTGCGAGAACGATATGAGATTCATCACAAGCTGCGTTATACTGATGAGGCATTGGTTTCTGCTGCTCAATTGTCCCATCAGTACATCAGTGATCGTTTCCTTCCCGATAAGGCAATTGATCTGATTGATGAGGCTGGTTCTCGAGTGAGATTGAGGCATGCACAGCTTCCCGAGGAGGCCAGAGAGCTTGAAAAAGAACTTAGACAAATAACAAAAGAAAAGAATGAAGCTGTTCGAAGCCAGGACTTTGAAAAGGCTGGAGAGCTACGTGACCGGGAGATGGAGTTGAAGGCACAAATTGTTGCTATAACAGAGAAGGGTAAA

Fln Alignment:

sp_v1.1_unigene33569___GAEPSNIRTQVVRMVGENRDDDRAVVGPGSGSNKTPTLEEYGTNLTKLAEEGKLDPVVGRQPQIERVTQILGRRTKNNPCLIGEPGVGKTAIAEGLAQRIASGDVPETIEGKKVITLDMGLLVAGTKYRGEFEERLKKLMEEVKQSDD-LILFIDEVHTLXXXXXXXXXXXXXXXLKPALARGELQCIGATTLDEYRKHIEKDPALERRFQPVQVPEPTVDETIQILKGLRERYEIHHKLRYTDEALVSAAQLSHQYISDRFLPDKAIDLIDEAGSRVRLRHAQLPEEARELEK-------ELRQITKEKNEAVRSQDFEKAGELRDREMELK
B8LQS4_______________GVSAARVKSEVEKLRGEGKKVDTA-----SGDTNFQALKTYGRDLVE--DAGKLDPVIGRDEEIRRVVRILSRRTKNNPVLIGEPGVGKTAVVEGLAQRIVRGDVPSNLLDVRLIALDMGALVAGAKYRGEFEERLKSVLKEVEDAQGKVILFIDEIHLVLGAGRTEGSMDAANLFKPMLARGQLRCIGATTLEEYRKYVEKDAAFERRFQQVYVAEPSVTDTISILRGLKEKYEGHHGVRILDRALVVAAQLSSRYITGRHLPDKAIDLVDEACANVRVQLDSQPEEIDTLERKRIQLEVEMHALEKEKDKASKARLVEVRKELDDLRDKLQ

SNPs (tot: 70)

Position	A %	C %	G %	T %	Probability
268	10			90	0.90298
269	90	10			0.90298
270	90	10			0.90298
272		90		10	0.90298
273	90		10		0.90298
274	90		10		0.90298
277		90	10		0.90298
278	90			10	0.90298
279	90	10			0.90298
280	88		11		0.923564
281	90	10			0.90298
284		10		90	0.90298
285	10		90		0.90298
434		80		20	0.995493
636	83		16		0.994512
637			83	16	0.994512
639		83	16		0.994512
640	83			16	0.994512
641	83		16		0.994512
642	85	14			0.993533
643		14	85		0.993533
644		14		85	0.993533
645	71	14		14	0.984337
646			14	85	0.993533
647			14	85	0.993533
649	85	14			0.993533
651	71	14		14	0.984337
652			85	14	0.993533
654		85	14		0.993533
655	14		14	71	0.984337
656	71	28			1.0
658		14	71		0.984337
659			85	14	0.993533
660		14	85		0.993533
661	14		85		0.993533
662	85	14			0.993533
664		14		85	0.993533
694		11	88		0.923564
695	88		11		0.923564
696	88			11	0.923564
697	88	11			0.923564
700			11	88	0.923564
710		11	88		0.923564
711	88			11	0.923564
713			88	11	0.923564
718			88	11	0.923564
719		11	88		0.923564
841		87	12		0.992554
843	87		12		0.992554
844	87		12		0.992554
959		87		12	0.992554
1015	71		28		1.0
1035		83		16	0.994512
1106		80		20	0.995493
1190	16		83		0.994512
1227	83			16	0.994513
1228	83		16		0.994512
1229	83		16		0.994512
1230	83		16		0.994512
1304	84			15	0.999986
1307		15	84		0.999986
1309		15		84	0.999986
1697		16	83		0.999991
1698	83		16		0.999991
1709	83	16			0.999991
1712	83			16	0.999991
1713		16	83		0.999991
1714	16		83		0.999991
1717		16	83		0.999991
1718	16		83		0.999991

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain