SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene32991

Length: 1113 nt

Origin of reads: Biogeco_1 Biogeco_2 Uagpf Uma

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v1.1_unigene32991 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Glycolate oxidase n=1 Tax=Zantedeschia aethiopica RepID=Q84LB8_ZANAE	-	-	84%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	84%
Blast2go	2-hydroxy-acid glycolate short chain alpha-hydroxy acid	-	-	90%

Source	GOs	Term	Type	Identity
Blast2go	glycolate oxidase activity	GO:0008891	Molecular Function	90%
Blast2go	cytosolic ribosome	GO:0022626	Cellular Component	90%
Blast2go	peroxisome	GO:0005777	Cellular Component	90%
Blast2go	chloroplast stroma	GO:0009570	Cellular Component	90%
Blast2go	membrane	GO:0016020	Cellular Component	90%
Blast2go	nucleus	GO:0005634	Cellular Component	90%
Blast2go	apoplast	GO:0048046	Cellular Component	90%

Source	InterPros	Term	Type	Identity
Blast2go	FMN-dependent dehydrogenase	IPR000262	-	90%
Blast2go	Alpha-hydroxy acid dehydrogenase, FMN-dependent	IPR012133	-	90%
Blast2go	Aldolase-type TIM barrel	IPR013785	-	90%

Full-Lengther Next Prediction

Fln status: C-terminus

Fln database: coniferopsida.fasta

Fln subject: A9NXW1

Fln msg: Separated hits, possible frame ERROR between 340 and 342, your sequence is shorter than subject: 284 - 367

Fln protein: PDGELATARAASAAGTIMTLSSWATSSVEDVSSTGQGIRFFQLYVLKDRYVVAQLVRRAENAGFKAIVLTVDTPRLGRREADIRNRFSLPPHLTLKNFEGLDLGTLEKTGGSxxVASYAANQTDRSLSWKDVKWLQTITNLPILVKGVMTAEDTRLAVQAGAAGIIVSNHGARQLDYVPATISCLEEVVKAARGQIPVFLDGGVRRGTDVFKALALGASGIFIGRPVVYSLAVEGEAGVRKVLQMLRDELELTMSLAGCCSVKDINRSYIQTETDMFRALSRL*

Protein Length: 285

Fln nts: TCCAGATGGAGAGTTGGCTACTGCAAGAGCTGCTTCAGCTGCTGGAACAATAATGACGCTTTCATCATGGGCTACATCAAGTGTTGAAGATGTTTCCTCAACTGGACAAGGCATCCGCTTTTTCCAGCTTTATGTACTCAAGGATAGATATGTCGTTGCACAACTTGTTAGAAGGGCAGAGAATGCTGGCTTTAAGGCAATTGTACTTACTGTAGATACACCTCGACTGGGGCGGAGGGAGGCTGATATTAGAAACAGATTCTCACTGCCCCCGCATCTGACTCTGAAGAATTTTGAGGGCTTGGATCTTGGTACATTAGAGAAGACGGGAGGTTCTGGxxxCGTAGCCTCATATGCTGCCAATCAAACTGACAGATCATTGAGTTGGAAGGATGTTAAGTGGCTGCAGACAATCACCAACTTACCAATCCTAGTGAAGGGAGTAATGACAGCTGAGGACACCAGGCTAGCAGTACAAGCAGGTGCTGCAGGGATAATCGTGTCCAACCATGGGGCTAGGCAACTTGATTATGTTCCAGCAACAATAAGCTGCCTGGAAGAGGTTGTAAAAGCTGCAAGAGGTCAAATTCCTGTGTTTCTTGATGGTGGAGTTCGCCGTGGCACAGATGTTTTCAAGGCACTGGCCCTTGGAGCCTCTGGTATTTTTATTGGAAGGCCTGTTGTCTATTCTTTGGCTGTAGAAGGAGAAGCGGGTGTAAGGAAAGTTTTACAAATGCTGCGGGATGAGCTTGAACTCACCATGTCTTTGGCTGGATGTTGTTCAGTAAAGGACATAAATCGCAGCTACATTCAAACGGAAACTGACATGTTTAGGGCTCTTTCAAGGCTCTGATCGGGTCTAATTCAATAGTTGTCTACGAACAAAATGCATATTAACATCCTAAGATAATCTGGATAAGATGTTTTTATTATTTGTTCTGCTAGTCAAAACCTGAAGCTTTGATCCCGAAAATTTACCCACATTTTTCTGTCTCATGTGGCAATCAATGCTTGAAACTATTGCAGATGACATTTGTTTCTCTGGCCCTAACAGTGAGTGAACCTTAAGTAGATTTCTCGTGGCAATTGGTATTGTGATATTATGTTCATACAAA

Fln Alignment:

sp_v1.1_unigene32991___PDGELXXXXXXXXAGTIMTLSSWATSSVEDVSSTGQGIRFFQLYVLKDRYVVAQLVRRAENAGFKAIVLTVDTPRLGRREADIRNRFSLPPHLTLKNFEGLDLGTLEKTGGSGxVASYAANQTDRSLSWKDVKWLQTITNLPILVKGVMTAEDTRLAVQAGAAGIIVSNHGARQLDYVPATISCLEEVVKAARGQIPVFLDGGVRRGTDVFKALALGASGIFIGRPVVYSLAVEGEAGVRKVLQMLRDELELTMSLAGCCSVKDINRSYIQTETDMFRALSRL
A9NXW1_______________PEGEFATARASSAAGTIMTLSSWATSSVEEVASTGPGIRFFQLYVYKNRHVVEQLVRRAERAGFKAIALTVDTPRLGRREADIKNRFSLPPYLTLKNFEGLDLGKMEKTADSGxLASYVAGQIDRSLSWKDVKWLQTITNLPILVKGVMTAEDTRLAVQAGVQGIIVSNHGARQLDYVPATISSLEEVVKAAQGRVPVFLDGGVRRGTDVFKALALGASGIFIGRPVVFSLAAEGEAGVRNVLQMLRDEFELTMALAGCCSVKEINRNYIQTEADMIRSISRL

SNPs (tot: 44)

Position	A %	C %	G %	T %	Probability
385	11		88		0.991528
410		77		22	0.999999
412	22	77			0.999999
413			22	77	0.999999
416			22	77	0.999999
417	22	77			0.999999
419	22			77	0.999999
420			22	77	0.999999
422		22		77	0.999999
425	88	11			0.991528
426	12		87		0.992554
427		62	37		1.0
428		75		25	1.0
429	62	37			1.0
431	62	37			1.0
433	88	11			0.991528
434	66	33			1.0
435	11		22	66	0.999999
437			85	14	0.993533
477		16	83		0.994512
529	87			12	0.992554
530		87	12		0.992554
662		7	92		0.999532
666	7	92			0.999602
668			11	88	1.0
669		92	7		0.999602
671	13		86		1.0
675	8		91		0.999807
676	8			91	0.999807
751		90	10		0.90298
754	10			90	0.90298
763	9		36	54	1.0
766	36		54	9	1.0
771	54	36			1.0
772	36		9	54	1.0
774		9	36	54	1.0
776	60			40	1.0
777		40	60		1.0
778	40		60		1.0
780			40	60	1.0
950		75	25		0.996473
988	57			42	1.0
1010		85		14	0.993533
1059		88		11	0.923564

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain