SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene30130

Length: 1700 nt

Origin of reads: Biogeco_2 Uma

UniGene Fasta

Ace file of the UniGene sp_v1.1_unigene30130

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Pectate lyase precursor, putative (EC:4.2.2.2)	-	-	62%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	80%
Blast2go	pectate lyase	-	-	81%

Source	InterPros	Term	Type	Identity
Blast2go	Pectate lyase/Amb allergen	IPR002022	-	81%
Blast2go	Pectin lyase fold/virulence factor	IPR011050	-	81%
Blast2go	Pectin lyase fold	IPR012334	-	81%
Blast2go	AmbAllergen	IPR018082	-	81%

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: B8LQH9

Fln msg: Overlapping hits, possible frame ERROR between 735 and 710,

Fln protein: MKNRSILTCVFLIEILCVDGGVVPNPFGILVAENSREAEPTNGNTTAVEKDPDSIVQMVQRSIDNSTRRRELGYLSCGTGNPIDDCWRCDPNWQHHRKRLADCAIGFGRHAKGGKKGKTYVVTDHHDYDAVNPRPGTLRHAVIxxxxxxxxxxQRDMVIQLKEELIMNSFKTIDGRGVDVHIAYGPCITIQHVSHIIIHGIHVHDCKPAGNAMVRDSPTHYGWRTMCDGDGISIFEGSHVWVDHVSLSKCTDGLIDAIMGSTAITVSNNHFTDHNEVMLLGHSDSYTPDVNMQITIAYNHFGEGLRQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQGNRFSAPADPFAKEVTKREDNNENEWRQWNWRSEGDMLLNGAFFVSSGAGAASAYAKASSLGARPSSLVQPLTATAGVLTCRRGVRC*

Protein Length: 432

Fln nts: ATGGGGGCATCAGAGTGAGAGAGAGAGCAGAGGCAATCACCACTAATGTTGGCCATGCTCTCGAGTCTCTCCTCATTCCCACGCTCTTCTTCTTGCTGTAGCTATTGCTTTGAATGCTAGCTTTTTCTTCTCGTTTTCATACTTTCTACTGCGTTTCATGTCATGGGAGAGTTTAGAACTGCAGCATCGCCTTTTCCTTCACACAAGATTTTCTATTATTCTTCACTTTTGAAGAACTGACTGTAGATTTGTGAGAACTAGTGAGTGCTGGGTAATAAG_-_ATGAAGAATCGGAGTATTTTGACCTGCGTGTTCTTGATCGAAATCCTCTGTGTCGATGGTGGTGTGGTACCAAATcCTTTTGGAATTCTGGTTGCAGAAAATTCAAGAGAAGCAGAGCCAACCAATGGCAATACCACTGCTGTGGAAAAAGATCCAGACAGCATAGtGCAGATGGTTCAAAGGAGCATTGATAACAGCACGAGGAGGCGAGAGCTGGGTTATCTGTCCTGCGGAACTGGTAACCCCATTGATGATTGCTGGCGTTGCGATCCCAATTGGCAACACCACAGGAAACGCCTGGCAGACTGCGCCATTGGATTCGGCAGGCATGCCAAGGGAGGCAAAAAAGGGAAAACTTACGTGGTAACGGATCATCATGATTATGATGCGGTAAATCCTCGGCCGGGCACCCTGCGTCATGCGGTGATTxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxCAGAGAGACATGGTCATCCAgCTCAAGGAGGAGCTtATTATgAACAGTTTCAAGACAATcGACGGAAGGGGTGTCGATGTCCACATTGCATACGGtCCgTGTATCACCATtCAGcaTGTGaGTcACATcATCATCCATGGCATTCACGTTCACGACTGTAAgCCTGCtGGAAATGCTATGGTcAGGGAtTCTCCgACGCATTaCGGGTGGAGAACcATGTGCGATGGAGATGGcATCTCCATCTTCGaAGGAAGCCAtgTTTGGGTGGAcCATGTCTCCTTGTCCAAaTGTaCtGACGGTCTCATTGATGCCATCATGGGATCCACgGCTATTACCgTtTCAAACAaCcATtTCACTgACCACAAcgAGGTGATGTTGTTGGGTCAtAGTGATAGCTATACTCCCGATGTcAACATGCAGaTCACGATTGCATatAAcCATTTTGGGGAAGGTCTTAGGCAGAGAATGCCCAGGTGCAGGCATGGATACTTCCATGTGGTAAACAATGACTACACTCACTGGGAAATGTACGCAATTGGGGGCAGTGCAAACCCGACAATCAACAGTCAAGGCAATAGATTCTCGGCACCTGCAGACCCCTTTGCCAAAGAGGTAACAAAGAGGGAAGACAACAATGAGAATGAATGGAGACAGTGGAACTGGAGATCGGAAGGAGATATGCTGCTGAACGGTGCATTTTTTGTTTCATCGGGAGCAGGGGCGGCCTCAGCGTATGCAAAAGCATCGAGTCTGGGAGCCAGGCCTTCCAGCCTCGTCCAACCGCTTACCGCGACTGCAGGTGTACTCACTTGCAGGAGAGGAGTTCGCTGCTAA___CACCCTCAACTACTAACCCATTTGCTGACCCACCAAGTTCAAGTTCAAGTTCAAGTTCAAGTTCAACCAACCAGTGAATGTGAAGTAATCATTATTTTGGCAATTAAGCAACTCCCATTAACGCCACGCC

Fln Alignment:

sp_v1.1_unigene30130___MKNRSILTCVFLIEILCVDGGVVPNPFGILVAENSREAEPTNGNTTAVEKDPDSIVQMVQRSIDNSTRRRELGYLSCGTGNPIDDCWRCDPNWQHHRKRLADCAIGFGRHAXXXXXXXTYVVTDHHDYDAVNPRPGTLRHAVIxxxxxxxxxQRDMVIQLKEELIMNSFKTIDGRGVDVHIAYGPCITXXXXXXXXXXXXXXXDCKPAGNAMVRDSPTHYGWRTMCDGDGISIFEGSHVWVDHVSLSKCTDGLIDAIMGSTAITVSNNHFTDHNEVMLLGHSDSYTPDVNMQITIAYNHFGEGLRQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQGNRFSAPADPFAKEVTKXXXXXXXXXXXXXXXSEGDMLLNXXXXXXXXXXXXXXXXXXXXXXXRPSSLVQPLTATAGVLTCRRGVRC
B8LQH9_______________MKNQSILTGMLLIGILCIHGDAAQKPFGSLVVENSGEAEPANGNTTAAEKDPDSIVQMVQRSIDNSTRRRELGYLSCGTGNPIDDCWRCDPNWENHRKRLADCAIGFGSHAQGGKKGKYYVVTDPSDFDAVNPLPGTQRHAVIxxxxxxxxxERDMVIQLKEELIMNSFKTIDGRGVNVHIAYGPCITIHRVRNIIIHGIHVHDCKPAGNAMVRDSPTHYGWRPICDGDGISISRARHIWVDHVSLSNCADGLIDAIRGSTAITISNSYFTHHNEVMLLGHHDNYTADVHMQVTIAFNHFGEDLVQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQGNRFLAPENRWAKEVTKRVNENKDEWKKWNWRSEGDMMLNGAYFTPSGAVASSAYAKASSLAARPSADVGPLTQDAGVLQCRSGARC

SNPs (tot: 35)

Position	A %	C %	G %	T %	Probability
142		66		33	0.997452
168	66		33		0.997452
238		75		25	0.996473
268			75	25	0.996473
270			25	75	0.996473
276	75	25			0.996473
291		33	66		0.997452
335		33	66		1.0
571	33		66		1.0
941		66		33	0.997452
944	33		66		1.0
962		14		85	0.993533
971	16	50	33		1.0
983		85		14	0.993533
985	50		50		1.0
986	85		14		0.993533
995		50		50	1.0
996	66		33		1.0
1007		40		60	1.0
1025	40	60			1.0
1029	40		60		1.0
1031	60	20		20	0.99255
1034		83		16	0.994512
1037	16			83	0.994512
1046		16		83	0.994512
1064			40	60	1.0
1070		20		80	0.995493
1074	75		25		0.996473
1076		75		25	0.996473
1084	75		25		0.996473
1086		75		25	0.996473
1089		50		50	1.0
1095		50	50		1.0
1103		75		25	0.996473
1104	50		50		1.0

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain