SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene30057

Length: 2345 nt

Origin of reads: Biogeco_1 Biogeco_2 Uagpf Uma

UniGene Fasta

>sp_v1.1_unigene30057
GATAGATATTGCGTGTCCCTATTGAAAGTTGGGGCCCATGGTGCATTATTATAAGAAATTTTTAGGCCCTGAATCCAACACCACCGTGAAGCGAATGTAGCAGTAATAGCAGATAATAGAAACAGCTACTCCAATGTTCCCTGCTGGCGAGGAGCTTGCAACTTTTCTTGTTTCATGCAGATTTCTGCTACCCAGGGCTTGGGAAGAGTCTCTTCATGCAAGCAGCTTAAATAGTTTTACTCTGAATGAGGATGATGATGTTTTATACGTTGCCTTCCCTTCATTTCATAGAATAGAAGATTTCATAGTCACTGAGAGCGAATATGGGGAGGGAAATATCCAAACTAGAAACGAGGTCTTCTCTGGTTGCCTTAAAGGTAATGATGATCAACTGGCACTTGTCCATCAAGGAGCCCTTAAGATCTTCCTGCATATCATGGAGAACACAGATTTCAAAACACAGTTGCAGATATATATCAACTCGAAACAAACGCAACCCAAACCCATTATATTTGTGGGCCATTCCCTAGGAGGGGCAGTTGCAACCTTGGTCACACTATGGGTACTGGAAAAGAAACTAAAGCAAAGCTCTCCTTTTTGTTACACTTTAAAGAATGTTTCTCCATTCTGTATCACATTTGGTTGCCCTTTAGTGGGAGATGAAAGACTTGTTCAAGCAATTGGCCGTGAGCATTGGGGAGGCAACTTTTGTCACGTGGTGTCCAAACATGATATTGTTCCACGGATGCTCCTTGCACCCTTGGAATCAATTTCTAAGCCCTTAATTGCTGTTTTTTCATATTGGCAGGGCATAGATGTACATGATACTTTTGTTCAAGATGCTTGTAGGACGCTTCTTACTAGTGTCCTTGATTCTCTAAGGGAGTCAGATGGGATTGTCACGAAAAGCCCTTACAGGCCCTTTGGCACTTATCTGTTTTGCTCAAGCAATGGTGCCACTTGCATTGAAAATCCTGAGACAGTTTTGACGATGCTTCACTGGACAATACAAAGCCAAGAAACTTCTTTGGATGAGATTGTACAGAACTGCCTTTTAGAGCACATGAGGTATGGTTCTTTCTTGAAGAATGTCAAACAAAATTCCATAAGAGGGAGAAGACTGGTGTATTCCAATTTAGAATCCTCTTACGATATGAGAGTTTCATTGCAGCTAGAAGCTATTGGTGTTGAAGTTCAGGATGATCCTGTAAAGCTGGCCTTCCTAAGCCTTGGGAAAACTGAAAACACACACAGCGTAGATGCTTCCAATCTAGCCATTAAGTTGAGCAAAAAAATTGTACCAGGGTTGAGCTCGAGTGGTATAAAGAATGTTGCGAGAGGGATGACATTGGTGGATATTATGATTCCTTCAAGAACCAAAATGACAAGAAGGATATCGATGCTAATGGGCGTAGATTAAAGCTCGGGGAGTTTTGGGATGAGATTATTGAAATGTGGGAGAGTCATGCTTTGCCCAGTGATTTCCGAACCCAAAATAAGTGGGTATATGCTAGCATGACATATAGGGAACTTGTAGAGCCTTTGGATATAGCATATTATTATCGCAAGTCTGAGGGCAAGGGTAATTATCTGAAAGATGGAAGACCCCATCGTCATAAAGTTCTTCAGAAATGGTTCGAAGAAAAAGAAAAAACTCGCAGTGCAGCAGGTCAGATAGTGAGGACAAAGCTACCGATCCTTATTCCGGACTCATGCTTTTGGGCACATGTAGAAGAGGCTCTGAAAGATCTCAAAGAAGGCCTACATCAAAGGAAAGAAAGTCTTCAAAAGTTTGAAGATTATGTGACAAAGCTGATCAATGATCGCAACATTTCCTCCTATGTTTTCTTCCAAAGAAGCAGCTTCATGAGATGGTGGCAAGAATACAAGCAAGTCCAATCCCCTGAATGGAAATTAACCTCCCAGTTGTATAAGATAATGGAAAGTGAAAGTTGGAAGGCATGAATTCACAGTCCAAAGCCCTAAATGGAAATTAACCTCCCTGTTGTATGAGAAAATGGAAAGTGCAAGTTGGAAGGGATGAATTCACAGGGTATCTATCCATCTGGGTGAAAAGACTCCGTCAGCCACTTTGAAGGAGAGGACATTCTTTCAATTAAATTTTATTTCGTGTGGATTAGTTGCATTTTTCATCCCTTCACTTGTTTAAATAACATGATGGCTGTTATTTCCCTATGTTGAAGGTGGACATGTACAGCATGATGGCTTAACATTTCCAAGGGGATCTACTCAGACCTTCAATGGCTTAACATTTGATAAATGTGCCTAACACAGTAACCTTAGTAGCTTAAGAAAGGAAGAAAATTTGATTGCTGTATGTCCTT

Ace file of the UniGene sp_v1.1_unigene30057

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	PAD4; PAD4 (PHYTOALEXIN DEFICIENT 4); lipase/ protein binding / triacylglycerol lipase	-	-	35%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	82%
Blast2go	phytoalexin-deficient 4-1 protein	-	-	65%

Source	InterPros	Term	Type	e value	Identity
Blast2go	Lipase, class 3	IPR002921	-	0.0	65%

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: B8LL55

Fln msg: Overlapping hits, possible frame ERROR between 1294 and 1282,

Fln protein: MFPAGEELATFLVSCRFLLPRAWEESLHASSLNSFTLNEDDDVLYVAFPSFHRIEDFIVTESEYGEGNIQTRNEVFSGCLKGNDDQLALVHQGALKIFLHIMENTDFKTQLQIYINSKQTQPKPIIFVGHSLGGAVATLVTLWVLEKKLKQSSPFCYTLKNVSPFCITFGCPLVGDERLVQAIGREHWGGNFCHVVSKHDIVPRMLLAPLESISKPLIAVFSYWQGIDVHDTFVQDACRTLLTSVLDSLRESDGIVTKSPYRPFGTYLFCSSNGATCIENPETVLTMLHWTIQSQETSLDEIVQNCLLEHMRYGSFLKNVKQNSIRGRRLVYSNLESSYDMRVSLQLEAIGVEVQDDPVKLAFLSLGKTENTHSVDASNLAIxxxxxxCTRVELEWYKECCERDDIGGYYDSFKNQNDKKDIDANGRRLKLGEFWDEIIEMWESHALPSDFRTQNKWVYASMTYRELVEPLDIAYYYRKSEGKGNYLKDGRPHRHKVLQKWFEEKEKTRSAAGQIVRTKLPILIPDSCFWAHVEEALKDLKEGLHQRKESLQKFEDYVTKLINDRNISSYVFFQRSSFMRWWQEYKQVQSPEWKLTSQLYKIMESESWKA*

Protein Length: 612

Fln nts: GATAGATATTGCGTGTCCCTATTGAAAGTTGGGGCCCATGGTGCATTATTATAAGAAATTTTTAGGCCCTGAATCCAACACCACCGTGAAGCGAATGTAGCAGTAATAGCAGATAATAGAAACAGCTACTCCA_-_ATGTTCCCTGCTGGCGAGGAGCTTGCAACTTTTCTTGTTTCATGCAGATTTCTGCTACCCAGGGCTTGGGAAGAGTCTCTTCATGCAAGCAGCTTAAATAGTTTTACTCTGAATGAGGATGATGATGTTTTATACGTTGCCTTCCCTTCATTTCATAGAATAGAAGATTTCATAGTCACTGAGAGCGAATATGGGGAGGGAAATATCCAAACTAGAAACGAGGTCTTCTCTGGTTGCCTTAAAGGTAATGATGATCAACTGGCACTTGTCCATCAAGGAGCCCTTAAGATCTTCCTGCATATCATGGAGAACACAGATTTCAAAACACAGTTGCAGATATATATCAACTCGAAACAAACGCAACCCAAACCCATTATATTTGTGGGCCATTCCCTAGGAGGGGCAGTTGCAACCTTGGTCACACTATGGGTACTGGAAAAGAAACTAAAGCAAAGCTCTCCTTTTTGTTACACTTTAAAGAATGTTTCTCCATTCTGTATCACATTTGGTTGCCCTTTAGTGGGAGATGAAAGACTTGTTCAAGCAATTGGCCGTGAGCATTGGGGAGGCAACTTTTGTCACGTGGTGTCCAAACATGATATTGTTCCACGGATGCTCCTTGCACCCTTGGAATCAATTTCTAAGCCCTTAATTGCTGTTTTTTCATATTGGCAGGGCATAGATGTACATGATACTTTTGTTCAAGATGCTTGTAGGACGCTTCTTACTAGTGTCCTTGATTCTCTAAGGGAGTCAGATGGGATTGTCACGAAAAGCCCTTACAGGCCCTTTGGCACTTATCTGTTTTGCTCAAGCAATGGTGCCACTTGCATTGAAAATCCTGAGACAGTTTTGACGATGCTTCACTGGACAATACAAAGCCAAGAAACTTCTTTGGATGAGATTGTACAGAACTGCCTTTTAGAGCACATGAGGTATGGTTCTTTCTTGAAGAATGTCAAACAAAATTCCATAAGAGGGAGAAGACTGGTGTATTCCAATTTAGAATCCTCTTACGATATGAGAGTTTCATTGCAGCTAGAAGCTATTGGTGTTGAAGTTCAGGATGATCCTGTAAAGCTGGCCTTCCTAAGCCTTGGGAAAACTGAAAACACACACAGCGTAGATGCTTCCAATCTAGCCATTxxxxxxxxxxxxxxxxxxTGTACCAGGGTTGAGCTCGAGTGGTATAAAGAATGTTGCGAGAGGGATGACATTGGTGGATATTATGATTCCTTCAAGAACCAAAATGACAAGAAGGATATCGATGCTAATGGGCGTAGATTAAAGCTCGGGGAGTTTTGGGATGAGATTATTGAAATGTGGGAGAGTCATGCTTTGCCCAGTGATTTCCGAACCCAAAATAAGTGGGTATATGCTAGCATGACATATAGGGAACTTGTAGAGCCTTTGGATATAGCATATTATTATCGCAAGTCTGAGGGCAAGGGTAATTATCTGAAAGATGGAAGACCCCATCGTCATAAAGTTCTTCAGAAATGGTTCGAAGAAAAAGAAAAAACTCGCAGTGCAGCAGGTCAGATAGTGAGGACAAAGCTACCGATCCTTATTCCGGACTCATGCTTTTGGGCACATGTAGAAGAGGCTCTGAAAGATCTCAAAGAAGGCCTACATCAAAGGAAAGAAAGTCTTCAAAAGTTTGAAGATTATGTGACAAAGCTGATCAATGATCGCAACATTTCCTCCTATGTTTTCTTCCAAAGAAGCAGCTTCATGAGATGGTGGCAAGAATACAAGCAAGTCCAATCCCCTGAATGGAAATTAACCTCCCAGTTGTATAAGATAATGGAAAGTGAAAGTTGGAAGGCATGA___ATTCACAGTCCAAAGCCCTAAATGGAAATTAACCTCCCTGTTGTATGAGAAAATGGAAAGTGCAAGTTGGAAGGGATGAATTCACAGGGTATCTATCCATCTGGGTGAAAAGACTCCGTCAGCCACTTTGAAGGAGAGGACATTCTTTCAATTAAATTTTATTTCGTGTGGATTAGTTGCATTTTTCATCCCTTCACTTGTTTAAATAACATGATGGCTGTTATTTCCCTATGTTGAAGGTGGACATGTACAGCATGATGGCTTAACATTTCCAAGGGGATCTACTCAGACCTTCAATGGCTTAACATTTGATAAATGTGCCTAACACAGTAACCTTAGTAGCTTAAGAAAGGAAGAAAATTTGATTGCTGTATGTCCTT

Fln Alignment:

sp_v1.1_unigene30057___IETATPMFPAGEELATFLVSCRFLLPRAWEESLHASSLNSFTLNEDDDVLYVAFPSFHRIEDFIVTESEYGEGNIQTRNEVFSGCLKGNDDQLALVHQGALKIFLHIMENTDFKTQLQIYINSKQTQPKPIIFVGHSLGGAVATLVTLWVLEKKLKQSSPFCYTLKNVSPFCITFGCPLVGDERLVQAIGREHWGGNFCHVVSKHDIVPRMLLAPLESISKPLIAVFSYWQGIDVHDTFVQDACRTLLTSVLDSLRESDGIVTKSPYRPFGTYLFCSSNGATCIENPETVLTMLHWTIQSQETSLDEIVQNCLLEHMRYGSFLKNVKQNSIRGRRLVYSNLESSYDMRVSLQLEAIGVEVQDDPVKLAFLSLGKTENTHSVDASNLAIKxxxxNCTRVELEWYKECCERDDIGGYYDSFKNQNDKKDIDANGRRLKLGEFWDEIIEMWESHALPSDFRTQNKWVYASMTYRELVEPLDIAYYYRKSEGKGNYLKDGRPHRHKVLQKWFEEKEKTRSAAGQIVRTKLPILIPDSCFWAHVEEALKDLKEGLHQRKESLQKFEDYVTKLINDRNISSYVFFQRSSFMRWWQEYKQVQSPEWKLTSQLYKIMESESWK
B8LL55_______________METATQTFTAGEEIATFLVSYRFLLHKAWEESLRANSSKSFTLNEHEDVVYVAFPSFHGIEDFMVIESEYGEGNIQTGNEVFSGCLKGNDDQLALVHQGALKIFLHIMENTDFKKKLQIYINSKQKKPKSIIFVGHSLGGAVATLVTLWVLEKRLKQSSPFCITLKDVNPFCITFGCPLVGDERLVEAVGREHWGGNFCHVVSKHDIVPRMLLAPLESIAQPLIAIFPYWQGIDAPDAFIQDACRTLLNNVFDSLRESNGVVKKSPYRPFGTYMFCSSNGAACIENSETVLKMLHWTIQSQETSLDEIVQDCLLEHIRYGSVLKIVMQNSIRGRKLVNFNSESSYEMRISLQLEAVG--VQDDHVKLDLLNLGRTENKHSADVSNLAIKxxxxNCTRVELEWYKECCEKDDIGGYYDSFKNQNDKRDIDANGRRLKLGEFWDELIEMWESHALPSDFRTQNKWVNAGMTYRELVEPLDIAYYYRKSEGKGNYLSDGRPHRHKVLQKWMEDKDKTREAEGRVARTKLPFLIPDSCFWAHVEEALKDLKQGLHQRKESLQKFEDYVTKLIDDRNISSYVFLERSSFMRWWQEYKQVQFPEWKLTSPLYKIMETASWK

SNPs (tot: 8)

Position	A %	C %	G %	T %	Probability
1129	20		80		0.999996
1220	83		16		0.994512
1244		33	66		0.997452
1267		66		33	0.997452
1282		33		66	0.997452
1430	80		20		0.995493
1916	80		20		0.995493
2179	66	33			1.0

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain