SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene29794

Length: 1703 nt

Origin of reads: Biogeco_1 Biogeco_2 Uagpf Uma

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v1.1_unigene29794 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	dead box ATP-dependent RNA helicase, putative	-	-	65%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	34%
Blast2go	dead box atp-dependent rna	-	-	80%

Source	InterPros	Term	Type	Identity
Blast2go	RNA helicase, ATP-dependent, DEAD-box, conserved site	IPR000629	-	80%
Blast2go	Helicase, C-terminal	IPR001650	-	80%
Blast2go	DNA/RNA helicase, DEAD/DEAH box type, N-terminal	IPR011545	-	80%
Blast2go	Helicase, superfamily 1/2, ATP-binding domain	IPR014001	-	80%
Blast2go	RNA helicase, DEAD-box type, Q motif	IPR014014	-	80%

Full-Lengther Next Prediction

Fln status: Putative Complete

Fln database: coniferopsida.fasta

Fln subject: B8LKZ7

Fln msg: STOP codon was not found. Distance to subject end: 12 aas, Unexpected STOP codon in 5 prime region, your sequence is shorter than subject: 469 - 504

Fln protein: MHMEEPEEETVPTAAVPSLFSKGALKKNKKNKFSNSEPPQTKLKLTNDKDYEGKSFEDLGLSEWAVHVCRELGMKKPTPVQQHCIPQIIKGKDVMGLAQTGSGKTAAFALPILHKLAENPYGVFALVITPTRELAFQLAEQFKAIGSPLRLRCTVVIGGMDMISQAQALMQRPHVVIATPGRIKSLLEDYPDVPKVFSRAKFLVLDEADRLLDVGFEEELKVVFQCLPTERQTLLFSATMTGDLRALHDLSGDKAYFYEAYEGFKTVESLNQQYVFVPENVKDVYLMHIVSTMEDNEIRSAIVFVSTCRTCHLLSLLLDELGLEAVALHSLKSQSARLAALQRFKSGQVPVLLATDVASRGLDIPTVDLVINYDIPRYPRDYVHRVGRTARAGRGGQAVSIVTQYQVKLVHDIEAVVGKQLEEYDCKEDDVLKGITQVYKAKRMATMRMVDDGFEAKAKERKEMKLKML

Protein Length: 470

Fln nts: CGTCATGACCTTGGCTGTCACTCAATATATCGTCGTGACCTTGGCTGTCACTCATTTTTTGAAGTGTCCGAGCTTGCAACCCTGCAAACGTATTTTCGTTCCACCTCTGCACATCACCTGAAAAGCGAACTTTTCAAGTGCCCGACCTTGCAACCTCGCAAACGTATTTTGATTCCGCTTCTGTACATCCACTAAAAAGCGCAAAGCGCACAGCGCAGGATGCTACAACACACAGCATAATCATGGACAAAATTTTCCTTCCATAGTCTAAGAGTAGAATATATATGTCTATAT_-_ATGCACATGGAGGAACCAGAGGAAGAAACGGTGCCAACAGCAGCAGTTCCATCCCTTTTCTCCAAAGGGGCACTGAAGAAAAACAAGAAGAATAAATTTTCCAATTCAGAGCCTCCACAGACCAAGCTCAAACTAACAAACGATAAAGACTATGAGGGTAAGAGCTTTGAGGATTTGGGTTTATCAGAATGGGCTGTACATGTATGCAGGGAGCTCGGGATGAAGAAGCCCACCCCGGTTCAACAGCACTGTATTCCTCAAATTATCAAAGGAAAAGACGTGATGGGTTTGGCCCAGACTGGCAGTGGTAAGACGGCCGCCTTTGCACTGCCCATACTGCATAAATTAGCGGAGAATCCATATGGCGTTTTTGCCCTGGTCATCACTCCTACCAGGGAGTTGGCTTTTCAGCTGGCCGAACAATTCAAAGCCATTGGATCACCTCTGCGTCTGCGGTGCACGGTCGTTATCGGTGGGATGGATATGATCAGTCAGGCCCAAGCCTTGATGCAGCGACCCCATGTTGTGATCGCCACCCCTGGGCGTATCAAGTCTCTCCTCGAGGACTATCCTGATGTCCCCAAAGTGTTTTCCAGGGCAAAGTTTCTTGTTTTGGATGAAGCAGATCGTTTACTGGATGTGGGTTTTGAAGAAGAACTGAAGGTAGTATTTCAGTGTTTGCCCACTGAACGTCAAACTCTTCTCTTTTCAGCGACAATGACTGGTGATCTAAGAGCCTTGCATGATTTATCTGGAGACAAAGCATACTTTTATGAGGCATACGAGGGATTTAAGACAGTTGAGTCATTGAACCAGCAATACGTTTTTGTTCCTGAAAATGTGAAGGATGTATATCTCATGCACATTGTTTCAACTATGGAAGATAATGAGATCAGATCTGCCATTGTTTTTGTTTCCACCTGCAGGACTTGCCATCTGCTTAGTTTATTGTTGGATGAACTTGGTTTGGAGGCAGTGGCATTGCATTCTCTCAAATCACAATCTGCAAGGCTTGCTGCTCTTCAGCGTTTCAAATCTGGGCAGGTTCCGGTTCTGTTAGCAACTGATGTAGCAAGTCGTGGTCTTGACATACCAACTGTTGATCTTGTCATCAATTATGATATACCAAGGTATCCTCGAGATTATGTTCATCGTGTAGGTCGTACAGCGAGAGCTGGAAGGGGAGGACAAGCTGTGAGCATAGTGACTCAGTATCAAGTGAAGCTTGTACATGATATAGAGGCTGTCGTTGGAAAACAGTTGGAGGAGTATGACTGTAAAGAGGATGATGTGCTGAAAGGTATCACACAGGTATATAAAGCCAAAAGGATGGCAACAATGAGGATGGTTGATGATGGTTTTGAGGCAAAAGCAAAAGAAAGGAAAGAAATGAAACTTAAAATGTTGGT

Fln Alignment:

sp_v1.1_unigene29794___KSFEDLGLSEWAVHVCRELGMKKPTPVQQHCIPQIIKGKDVMGLAQTGSGKTAAFALPILHKLAENPY-----GVFALVITPTRELAFQLAEQFKAIGSPLRLRCTVVIGGMDMISQAQALMQRPHVVIATPGRIKSLLEDYPDVPKVFSRAKFLVLDEADRLLDVGFEEELKVVFQCLPTERQTLLFSATMTGDLRALHDLSGDKAY-FYEAYEGFKTVESLNQQYVFVPENVKDVYLMHIVSTMEDNEIRSAIVFVSTCRTCHXXXXXXXXXXXXXXXLHSLKSQSARLAALQRFKSGQVPVLLATDVASRGLDIPTVDLVINYDIPRYPRDY
B8LKZ7_______________KNFGDAGFPDYVIEEIVKAGFTEPTPIQAQGWPMALKGRDLIGIAETGSGKTLAYLLPAIVHVNAQPYLAPGDGPIVLVLAPTRELAVQIQQEAAKFGASSKIKNTCIYGGAPKGPQVRDLSKGVEIVIATPGRLIDMLESQ---HTNLRRVTYLVLDEADRMLDMGFEPQIKKIVSQIRPDRQTLYWSATWPKEVELLARQSLHNPYKVIIGSSDLKANHAIEQIVEIVSEHEKYTRLIQLLEEIMDGS--RLLIFLETKKGCDQVTRKLRMDGWPALSIHGDKSQAERDWVLSEFKAGKSPIMTATDVAARGLDVKDIKCVINYDFPGSMEDY

SNPs (tot: 29)

Position	A %	C %	G %	T %	Probability
832		25		75	0.996483
851		50		50	1.0
1096		88		11	0.991528
1098	11		88		0.991528
1099		88	11		0.991528
1100		88		11	0.991528
1102	11			88	0.991528
1103		77		22	0.999999
1105	11			88	0.991528
1106	88		11		0.991528
1110	12	87			0.992554
1111			12	87	0.992554
1113		87		12	0.992554
1118	12			87	0.992554
1143		20		80	0.995493
1279	20		80		0.995493
1599		10		90	0.90298
1658	62	25		12	1.0
1659	62	25	12		1.0
1660	62	25		12	1.0
1661	55	22		22	0.999999
1662	66			33	1.0
1665			60	40	1.0
1670	60		40		1.0
1673			60	40	1.0
1675	33	66			1.0
1679	33		66		1.0
1681		66	33		1.0
1684			66	33	1.0

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain