SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene29357

Length: 1448 nt

Origin of reads: Biogeco_2 Uagpf Uma

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v1.1_unigene29357 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Monoglyceride lipase, putative (EC:3.1.1.23)	-	-	61%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	83%
Blast2go	esterase lipase thioesterase family protein	-	-	80%

Source	InterPros	Term	Type	e value	Identity
Blast2go	Alpha/beta hydrolase fold-1	IPR000073	-	0.0	80%
Blast2go	Alpha/beta hydrolase, N-terminal	IPR022742	-	0.0	80%

Full-Lengther Next Prediction

Fln status: Putative Complete

Fln database: coniferopsida.fasta

Fln subject: A9NXA7

Fln msg: atg_distance in limit (1-15): atg_distance = 8, Unexpected STOP codon in 5 prime region, your sequence is shorter than subject: 295 - 346

Fln protein: MECSVFMEDTGVRLAKAGYAVFGIDYEGHGKSGGTRCYIQSFDDLVTDCATFFRSVAENIKYKKKARFLYGESMGGVVALLIHRKQPNFWNGALLVAPMCKIAEELKPHPLVIGILKKLAVIIPTWKIVPIQNIIDTGFKEPEKRQKIRANPYIYQDKPRLKTGYELLMASLDIEKRLEEVTLPFLIVHGQDDKVTDPSVSQLLYSSAKSSDKTLKLYPDMWHGLTFGEPPEHVELVFSDIIAWLGERSEAAGRCHENAHFQKSSVSESAMMSAERERKLSNDSLSSKKVHNVA*

Protein Length: 296

Fln nts: CCTTAATCTTAGTCAGGATTCCATCACTCTGTAAATTTGTTAAAGTGATTGAGGATTCCATGACTCTGTAAATTTGTTAAAGATTTTTGGCTTCTATGGTTTAATTTCTTTCTTTTTCCTGTTGGTAGGCTATGCC_-_ATGGAGTGCAGCGTTTTCATGGAAGACACAGGAGTGAGGCTAGCAAAGGCTGGGTATGCAGTATTTGGTATAGATTATGAAGGACATGGAAAATCTGGTGGAACACGTTGTTACATACAAAGCTTCGATGACCTTGTAACGGATTGTGCAACTTTTTTCAGAAGCGTAGCTGAGAATATTAAGTATAAAAAGAAAGCGAGGTTCCTTTACGGTGAATCAATGGGAGGAGTTGTGGCACTGCTCATCCACAGAAAGCAGCCTAACTTTTGGAATGGTGCTCTACTTGTTGCCCCTATGTGCAAGATTGCAGAAGAGTTAAAGCCCCACCCCCTGGTTATCGGCATATTGAAAAAGTTAGCTGTCATAATCCCGACATGGAAGATTGTACCTATTCAGAACATTATAGATACGGGCTTTAAGGAACCAGAAAAGAGACAAAAGATTCGAGCAAATCCCTATATTTATCAAGACAAGCCTCGTCTAAAGACGGGTTACGAGCTCCTCATGGCCAGTTTGGACATAGAGAAACGGCTGGAGGAGGTGACCTTGCCTTTTCTGATTGTTCATGGCCAAGACGATAAAGTAACAGACCCATCTGTGAGCCAATTGCTGTACTCTTCGGCCAAAAGTTCTGACAAGACGCTGAAGCTGTACCCTGATATGTGGCATGGTTTGACTTTTGGCGAACCCCCTGAGCACGTTGAATTGGTGTTCTCAGATATCATTGCATGGTTGGGCGAGAGAAGTGAAGCAGCAGGCCGGTGCCATGAAAACGCCCACTTCCAAAAGTCGAGCGTTTCTGAATCGGCCATGATGTCGGCAGAAAGAGAAAGAAAGCTTTCCAACGACAGCCTGTCTTCAAAGAAAGTACACAACGTAGCTTGATGTGAAAAACTGGGAAAAACGAACTGGTTATAAGGTATTGGTATGAAAATGTTTTCTGGGTTTTACACCATCACAACTTAACTTTCATTCAACCGACACCGGGGCAATCCGCCGCATCTAAATAACTTTCATGTAACCGAACAGGGTGATTGAAAAAGTTCAAAAAGACATTGGATCTGCGTCAGTGGGGTCCAATTATGGTGGCTGTAATAGTTTAGAGGGTTCTGTTAACTAGTTATAATGAGGGTAGGTGGGTACGGCCGATCAGAAAACTGTACAGAGGACAGCCAGGCAGATCAGCATAAGGATCTGCACCATTGACATCGCGCAATGCACGGTTAGCAGATGTCTGCCCTGGGTGATTGACATTGTATCCATCTTACTATTCAATAACATGCCAAATTGTAATATGTTTCGATTGTCAAAA

Fln Alignment:

sp_v1.1_unigene29357___GYAMECSVFMEDTGVRLAKAGYAVFGIDYEGHGKSGGTRCYIQSFDDLVTDCATFFRSVAENIKYKKKARFLYGESMGGVVALLIHRKQPNFWNGALLVAPMCKIAEELKPHPLVIGILKKLAVIIPTWKIVPIQNIIDTGFKEPEKRQKIRANPYIYQDKPRLKTGYELLMASLDIEKRLEEVTLPFLIVHGQDDKVTDPSVSQLLYSSAKSSDKTLKLYPDMWHGLTFGEPPEHVELVFSDIIAWLGERSEAAGRCHENAHFQKXXXXXXXXXXXXXXRKLSNDSLSSKKVHN
A9NXA7_______________GYGMECSIFMEDTGVRLAKAGYAVFGIDLEGHGKSAGTRCYIKNFDDLVMDSVTFFRSVAESIEYREKARFLYGESMGGVVALLIHRKQPNYWNGAVLVAPMCKIAEEIKPHPLVISILKKLSLIIPTWKIVPTENIIDSAFKDPEKRQKIRANPYIYQDKPRLKTGYELLVTSLDMEKRLDEVSLPFLVVHGEDDKVTDPSVSKLLYTSAKSSDKTLKLYPDMWHGLTYGEPPEHIELVFSDIVAWLGKRSGAAGRCPEDAHLEKSSVAEYAMMSAESERKLANDNLSSKTLHN

SNPs (tot: 23)

Position	A %	C %	G %	T %	Probability
119	16	83			0.994512
134	60	40			1.0
165			66	33	1.0
182	33			66	1.0
368	85		14		0.993533
427		14		85	0.993533
574		22		77	0.999999
949		90		10	0.90298
963		90		10	0.90298
1038	77		22		0.999999
1051	11			88	0.923564
1082	14		85		0.993533
1088		85		14	0.993533
1147		25		75	0.996473
1344	66			33	0.997467
1361	66		33		0.997467
1363		66		33	0.997453
1364	66			33	0.997453
1365		33		66	0.997464
1366	66			33	0.997464
1367		33	66		0.997464
1368	66			33	0.997464
1370			66	33	0.997464

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain