SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene28799

Length: 1755 nt

Origin of reads: Biogeco_1 Biogeco_2 Uagpf Uma

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v1.1_unigene28799 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	afc, putative (EC:2.7.12.1)	-	-	71%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	38%
Blast2go	protein kinase	-	-	84%

Source	GOs	Term	Type	Identity
Blast2go	mRNA processing	GO:0006397	Biological Process	84%
Blast2go	protein binding	GO:0005515	Molecular Function	84%
Blast2go	protein kinase activity	GO:0004672	Molecular Function	84%

Source	InterPros	Term	Type	Identity
Blast2go	Protein kinase, catalytic domain	IPR000719	-	84%
Blast2go	Serine/threonine- / dual-specificity protein kinase, catalytic domain	IPR002290	-	84%
Blast2go	Serine/threonine-protein kinase, active site	IPR008271	-	84%
Blast2go	Protein kinase-like domain	IPR011009	-	84%
Blast2go	Serine/threonine-protein kinase-like domain	IPR017442	-	84%
Blast2go	Tyrosine-protein kinase, catalytic domain	IPR020635	-	84%

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: B8LL66

Fln msg: Separated hits, possible frame ERROR between 478 and 480,

Fln protein: MACVAKYHRPPHMLDENPRKRRKLTSSWDVLPSQLQVQPTLVFTGKIFGIGSRDEGENAPLIGGAEPSTSGSDNSRSTSSNIKPKEEGFRTGSPPWRSxxDKDGHYMFELGENLTPRYKILSLMGEGTFGQVLECWDRKSKDLVAIKIVRGLQKYREAAMIEVDVLRKVGKYDRNGTRCVQMREWFDYRNHTCIVFEKLGPSLYDFLRKTNYSPFPIDLVREVGRQLLESVAYMHELKLVHTDLKPENILFVSSDSVKVPDCKGVKGFPDYFKRLPKSSAIKLIDFGSTTFEDQDHTSVVSTRHYRAPEVIFLGLGWNYSCDIWSVGCILVELCSGETLFQTHENLEHLAMMERVMGPLPHRMIKRADGRCAKYFTRSRTQLLWPEQTSSRESIRAVRKLPKLRDLIMQNADHSAGLLIDLLQGLLKFEPSERLKASEALRHPFFTKDSRRFENIAYNSRVCK*

Protein Length: 465

Fln nts: CGTCGTGACCTTGGCTGTCACTCATAACTGTCTCGTTTCATAGCATAACGTAGTTGTTGTTTCGGCCTGTGAAACCCTATCTTCTTATTTTCTACCTGCAAACCCTAGAAACTCTAGCCCTGTCGATTAAATTTCTTCGGCTGGTGTTGCCACCGAAGGATTCAGAGCGCGTGGGGCAGCC_-_ATGGCTTGTGTTGCAAAATATCATCGTCCTCCTCATATGCTTGACGAAAACCCCAGGAAGCGCCGCAAGTTAACGTCATCGTGGGACGTCCTTCCGTCACAGCTTCAGGTCCAGCCAACACTAGTATTTACTGGGAAAATTTTTGGCATCGGAAGTAGAGATGAAGGAGAGAACGCCCCTTTGATAGGTGGGGCAGAACCCTCCACCTCCGGTTCAGACAATAGTCGGTCTACCTCTTCCAATATAAAGCCCAAAGAAGAGGGCTTCCGCACTGGCTCACCACCATGGCGCAGTGAxxxTGACAAGGATGGCCACTACATGTTCGAACTTGGAGAGAATTTGACGCCTCGATACAAAATACTCAGTCTGATGGGTGAAGGAACATTTGGTCAAGTGTTGGAATGTTGGGATCGAAAATCAAAGGATCTTGTTGCCATAAAAATTGTCCGTGGATTACAGAAGTACCGAGAGGCAGCAATGATAGAAGTCGATGTACTTCGTAAAGTCGGCAAATATGACAGGAATGGCACCCGCTGTGTGCAGATGCGCGAATGGTTTGACTACCGGAATCACACTTGCATAGTCTTTGAGAAGCTTGGCCCAAGCTTATATGATTTCCTACGCAAAACCAATTACAGCCCATTTCCCATTGATCTTGTACGGGAGGTTGGAAGGCAACTGTTGGAATCTGTAGCATATATGCATGAGTTGAAACTCGTTCACACGGATTTAAAGCCTGAAAATATACTTTTTGTTTCTTCGGATTCTGTGAAGGTTCCAGATTGCAAGGGTGTGAAGGGATTTCCAGATTACTTTAAAAGGTTGCCAAAGTCAAGTGCAATAAAGTTAATTGATTTTGGAAGCACCACATTTGAGGATCAAGATCACACCTCAGTGGTCTCTACAAGGCATTATCGAGCTCCTGAAGTTATTTTTTTGGGACTTGGATGGAACTACTCTTGTGATATTTGGAGTGTAGGGTGCATACTTGTGGAGCTATGCTCGGGTGAGACTTTGTTTCAAACTCATGAAAACTTGGAACATCTTGCTATGATGGAAAGGGTTATGGGGCCTTTGCCTCATCGTATGATAAAGAGAGCAGATGGAAGATGTGCGAAATATTTTACTAGAAGTCGAACACAATTGTTATGGCCCGAACAAACTTCGTCTCGAGAGAGCATCAGAGCAGTGAGGAAGTTACCAAAACTTAGGGATTTGATAATGCAGAACGCAGACCATTCTGCAGGTCTTCTTATAGACTTGTTGCAAGGCCTTCTTAAGTTTGAACCTTCAGAACGCTTGAAAGCTTCAGAGGCTTTGAGACATCCATTCTTCACAAAAGACTCGAGAAGATTTGAAAACATTGCTTATAATTCACGAGTATGCAAGTGA___AAATTGTTTGGTTCTCTATCTGTGAATATCAGGTTGGCTTTGTGGCCAGGAGTTGCAGAGAATGATTGTGCACAATGCCCAATATTCGAATGCTGTTCCAGCTGCTGGCACCTATCTAACTGGTGATGTATCATAATATAGAGTTGGATGTGGCTGAAAGAGTTTTGAAATGGTTGAAATACCA

Fln Alignment:

sp_v1.1_unigene28799___MACVAKYHRPPHMLDENPRKRRKLTSSWDVLPSQLQVQPTLVFTGKIFGIGSRD-----EGENAPLIGGAEPXXXXXXXXXXXXXNIKPKEEGFRTGSPPWRSDxDKDGHYMFELGENLTPRYKILSLMGEGTFGQVLECWDRKSKDLVAIKIVRGLQKYREAAMIEVDVLRKVGKYDRNGTRCVQMREWFDYRNHTCIVFEKLGPSLYDFLRKTNYSPFPIDLVREVGRQLLESVAYMHELKLVHTDLKPENILFVSSDSVKVPDCK-GVKGFPD---YFKRLPKSSAIKLIDFGSTTFEDQDHTSVVSTRHYRAPEVIFLGLGWNYSCDIWSVGCILVELCSGETLFQTHENLEHLAMMERVMGPLPHRMIKRADGRCAKYFTRSRTQLLWPEQTSSRESIRAVRKLPKLRDLIMQNADHSAGXXXXXXXXXLKFEPSERLKASEALRHPFFTKDSRRFENIAYN
B8LL66_______________MACVAEY--PSYFIEGHPRKRRKLTASWDTRHYQDQAQQSVYFSGQGVGNGNGNGNGSGNGNGIPSSAGAS----SSSYHYCSSSSLYSKRVTARNASPPWRDDxDKDGHYMFALGDNLTPRYKILNKIGEGTFGQVLECWDRETREFVAVKIVRGLQRYREAAMIEIDVLREVAKYDRSGTRCVQMRNWFDYRNHVCIVFEKLGPSLYDFLRKNNYHPFPIDIVRELGKQLLESVAYMHHLHLIHTDLKPENILFASSEYKKVPYYKNGLKRLSQDGTCYMRLPKSTAIKLIDFGSATFEDQNHSSIISTRHYRAPEVI-LGLGWSYPADVWSIGCILVELCVGETLFQTHENMEHLAMMERVLGPFPQHLIRKADARSAKYF-RHGTRLNFPEGASSRESIRAVKKLPRLRNLVMERADHSAGALIDLLQGLLKYEPSERLEAQEALRHPFFTEDYRGFGSGVFN

SNPs (tot: 27)

Position	A %	C %	G %	T %	Probability
479	50	50			1.0
481		50		50	1.0
483		50		50	1.0
486		50		50	1.0
488	50			50	1.0
493		50		50	1.0
498	50			50	1.0
510		50		50	1.0
512	50			50	1.0
513	50			50	1.0
642		66		33	0.997452
643	66		33		0.997452
644	66	33			0.997452
645	33	33			0.997753
795			66	33	0.997452
1525	12	87			0.992554
1540	85		14		0.993533
1708	60	40			1.0
1709	80	20			0.995493
1711		20		80	0.995493
1713		80		20	0.995493
1726	75	25			0.996473
1738	66	33			0.997452
1740		33		66	0.997452
1741		33	66		0.997452
1742	66	33			0.997452
1743	33		66		0.997452

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain