SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene28083

Length: 1498 nt

Origin of reads: Biogeco_1 Biogeco_2 Uma

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v1.1_unigene28083 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Heat shock protein 70 n=1 Tax=Camellia sinensis RepID=B3GPH1_CAMSI	-	-	61%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	68%
Blast2go	heat shock protein 70	-	-	77%

Source	InterPros	Term	Type	Identity
Blast2go	Heat shock protein Hsp70	IPR001023	-	77%
Blast2go	Heat shock protein 70	IPR013126	-	77%
Blast2go	Heat shock protein 70, conserved site	IPR018181	-	77%

Full-Lengther Next Prediction

Fln status: N-terminus

Fln database: coniferopsida.fasta

Fln subject: A9NXU2

Fln msg: Separated hits, possible frame ERROR between 481 and 496, Distance to subject end: 172 aas, your sequence is shorter than subject: 483 - 651

Fln protein: MASRHNQNGKAIGIDLGTTYSCMGVWLGDRASVDIITNEQGNRTTPSMVAFTDSERLIGEPAKNQIFYNAANTVFDAKRLIGRRFSDSSVQADRRLWPFSVVCGSNDKPLIQVTFKGENKTFMAEEILSMLLFKMKECAESCLxxxxxxAVITAPAYYNNSQRQATKAAGKIAGFEVLQILNEPTAAAVAYGFVRSAYSSEDGEKILIFDLGGGTFDVSVLNIKKHKIEVKAVGGDTHLGGEDFNDRMVNHFAQEFQKKHKIDIFSDAKALRRLRSACEKAKRVLSSSFETIVDIPCLHESRDLCCKIRRARFEDMNMDLFQKCISIVEKCLGDAGMSKTQIEKIVLVGGSTRIPKVQELLKAFFDGQKELCKSIHPDEAVAYGASVQAAVLNGDNTANLILRDVTPLSLGVEIQGDMMSVLVPRNTQIPVKREDIYETVEDNQESATVCVYEGERPIVEHNNLLGEFDLGGLPSAPRGDVEI

Protein Length: 484

Fln nts: ATTGGGGTTTGTCTACCCTCGTCGCCTTCTGGCTTGATTGTTTCTATCC_-_ATGGCTTCGCGCCATAATCAAAACGGCAAGGCTATCGGCATCGATCTCGGAACCACATACAGCTGTATGGGTGTTTGGCTCGGTGATCGGGCTAGTGTGGATATCATTACCAATGAGCAGGGAAATAGGACTACTCCATCCATGGTTGCCTTCACAGACAGTGAACGCCTCATTGGTGAACCTGCAAAGAATCAAATCTTTTACAACGCTGCAAACACAGTTTTTGATGCCAAGAGGTTGATTGGGAGGCGTTTCTCCGATTCCTCTGTTCAAGCTGACAGGCGCCTTTGGCCATTTTCTGTCGTGTGCGGATCCAATGACAAGCCCTTGATCCAGGTAACATTTAAGGGAGAAAATAAAACTTTTATGGCAGAGGAAATATTGTCAATGTTATTGTTTAAGATGAAAGAATGCGCTGAATCATGCCTGGGxxxxxxxxxxxxxxxGGCTGTCATTACCGCTCCTGCTTATTATAACAATTCTCAGAGGCAAGCTACTAAGGCCGCCGGGAAGATAGCAGGTTTTGAAGTGTTACAAATATTGAACGAGCCAACTGCTGCTGCAGTTGCATATGGATTTGTCAGGAGTGCTTATTCATCAGAAGATGGGGAGAAAATCTTAATTTTCGATTTGGGCGGAGGGACTTTCGATGTTTCTGTGCTTAATATTAAGAAGCACAAAATCGAGGTCAAGGCGGTCGGAGGAGATACCCATCTGGGAGGAGAGGATTTTAACGATAGAATGGTCAACCATTTTGCGCAGGAATTTCAGAAAAAACACAAAATCGACATATTCTCAGATGCAAAAGCGCTAAGGAGACTTCGCAGTGCATGCGAGAAGGCCAAGAGAGTGCTGTCTTCCTCTTTCGAGACCATTGTTGATATACCCTGTCTCCATGAATCCCGAGACCTTTGCTGTAAAATACGGAGGGCAAGGTTTGAGGACATGAATATGGATCTGTTTCAAAAATGCATTTCAATTGTTGAAAAGTGCTTGGGGGATGCTGGAATGAGTAAAACCCAAATTGAGAAAATTGTGCTGGTGGGAGGTTCTACGCGAATACCCAAAGTGCAGGAGTTGCTTAAAGCTTTCTTCGACGGCCAGAAGGAGTTGTGTAAGTCCATCCATCCTGATGAAGCTGTAGCTTATGGTGCTTCCGTGCAGGCTGCGGTGTTGAATGGCGACAACACAGCAAACCTCATACTTCGAGATGTCACTCCCCTTAGTCTAGGGGTTGAAATTCAAGGGGATATGATGAGCGTATTGGTCCCTAGAAATACACAAATTCCGGTTAAAAGGGAGGACATATATGAGACTGTAGAGGACAATCAGGAAAGTGCAACAGTATGTGTTTATGAAGGGGAAAGGCCTATTGTTGAACACAATAATCTTTTGGGGGAATTTGATTTGGGGGGACTTCCTTCTGCACCTAGAGGCGATGTCGAAATCA

Fln Alignment:

sp_v1.1_unigene28083___GKAIGIDLGTTYSCMGVWLGDRASVDIITNEQGNRTTPSMVAFTDSERLIGEPAKNQIFYNAANTVFDAKRLIGRRFSDSSVQADRRLWPFSVVCGSNDKPLIQVTFKGENKTFMAEEILSMLLFKMKECAESCLGxxxxxAVITAPAYYNNSQRQATKAAGKIAGFEVLQILNEPTAAAVAYGFVRSAYSSEDGEKILIFDLGGGTFDVSVLNIKKHKIEVKAVGGDTHLGGEDFNDRMVNHFAQEFQKKHKIDIFSDAKALRRLRSACEKAKRVLSSSFETIVDIPCLHESRDLCCKIRRARFEDMNMDLFQKCISIVEKCLGDAGMSKTQIEKIVLVGGSTRIPKVQELLKAFFDGQKELCKSIHPDEAVAYGASVQAAVLNG---DNTANLILRDVTPLSLGVEIQGDMMSVLVPRNTQIPVKREDIYETVEDNQESATVCVYEGERPIVEHNNLLGEFDLGGLPSAPRGDVEI
A9NXU2_______________GKAIGIDLGTTYSCVGVWQHDR--VEIIPNDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPQNTVFDAKRLIGRRITDASVQSDMKLWPFKVLSGPGDKPMIEVIYKGEKKQFSPEEISSMVLIKMREIAEAFLGxxxxxAVVTVPAYFNDSQRQATKDAGLIAGLNVLRIINEPTAAAIAYGLDKKATSTKE-KNVLIFDLGGGTFDVSILTIEEGIFEVKATAGDTHLGGEDFDNRMVNHFVQEFKRKHKKDISGNARALRRLRTACERAKRTLSSTTQTTIEIDSLYEGIDFYATITRARFEELNMDLFRRSMEPVEKCMRDAKMDKSQIDDVVLVGGSTRIPKVQQLLQDFFNG-KELCKSINPDEAVAYGAAVQAAILSGEGNEKVQDLLLLDVTPLSLGLETAGGVMTVLIPRNTTIPTKKEQIFSTYSDNQPGVLIQVYEGERARTKDNNLLGKFELTGIPPAPRGVPQI

SNPs (tot: 19)

Position	A %	C %	G %	T %	Probability
913		25		75	0.996473
1005	33			66	0.997452
1007	66			33	0.997452
1008	33		33	33	0.997753
1009			33	66	0.997452
1013	33			66	0.997452
1014		33	66		0.997452
1018	66		33		0.997452
1111			20	80	0.995493
1112	20			80	0.995493
1121		40		60	1.0
1238		25		75	0.996473
1247		25		75	0.996473
1278	80		20		0.995493
1322		20		80	0.995493
1325		20		80	0.995493
1377	75	25			0.996473
1378	75	25			0.996473
1381		75		25	0.996473

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain