SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene27800

Length: 2396 nt

Origin of reads: Biogeco_1 Uagpf Uma

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>sp_v1.1_unigene27800
CGTCGTGACCTTGGCTGTCATGGGGATTTTACCAAAACACAGCTGAATCCAAATATACGTGGCGGATTAGGGTTTTGAGCATTCGCAAAGGATTTTTATGCTCGTAGGTCTCGAGTTCAGGCTTTTCTTGTTTTATTTACATGCAGTTTGGCACCTTTCTGATAGCATATCACTTCTTGACTAGAGCTCTATATTACTATGCTCAGGTATTTTGGCTGAGAAGTGCGCAGAGATGTTTTGAACGCTAAATGATATACTTCACAAATGAACTCACAGACCCAAATTCAGAACATCAATTCCGCAGAATTTGAGCCTCTGAGAATTGGGCAAATAACATCTGAAAGCCTAAAGAAGTAACTAACATCCTCAGGAATGGCGTATGCAATGCACGGTTTGCTCTTTGCGTCTTTCCATCCCAGAAACTTCTCCATGAAACCTTGGCAGTCCAAATCCATGCTTAACAGGAAGCTCCTCGTGGGGAATGGCTGGCTGAAAATGCAGAATAAGCGTTGGAAAGGGTTTTCAGTTGTGTTTGAGATGGAGAATTCCCCGGCAAACGTTAATACAGAATCGGCAGCAACAAAATCTGATGCCATTTCAGCCATAGCCGGCGACGGAATCAGGGTTTGGAGCGCCGAGTCGAGCTTGATGGAGATTGATACCGTGAGCGAAGCGGAACTGAAAGAAAATGGTTTTCGTAGCACGCGGAGAACGAAGATTGTCTGTACAATTGGCCCTGCGTCCTGTCAATTTGATCAGCTTGAAGCCCTTGCCGTGAATGGAATGAACGTTGCCAGAATTAATATGTGCCATGGTACGAGAGAATGGCACCGCAGGGTCATTAGGAATGTCAGGAAACTGAATGATGAGAAGGGCTATGCGGTCGCTATTATGATGGATACTGAAGGGAGTGAAATTCACATGGGTGACCTCGGAGGTGCCCCTTCTGTTAAAGCCGAGGATGGTGAGGTTTGGACTTTCAGTGTGCGAGATTTCAAAGTGCCTCTCCCTGCTCATACTATTCATGTCAGCTATGATGGATTTGCAGAGGATGTTAAGGTTGGCGATGAGCTACTTGTGGATGGTGGCATGGTGAGGTTTGAGGTGGTTGAAAAACTTGGACCAGATGTGAAATGTCAGTGTACTGATCCTGGGTTGCTGTTACCACGTGCCAATATAACATTTTGGAGGCAGGGCAGGTTGGTGCGTGAAAGAAATGCCATGCTCCCCACAATTTCCTCCAAGGACTGGCTTGATATAGATTTTGGGATTTCCGAAGGGGTGGATTTCATTGCTATATCTTTTGTGAAATCAGCAGAGGTGATCAAACATTTGAAGAGCTACATTGCAGCTCGATCTCGTGATGGTGAAATTGGTGTCATTGCGAAGATCGAAAGTGTTGATTCTCTAAAGTTTCTTGAAGAGATAATTCACATATCTGATGGAACGATGGTAGCGAGAGGGGACCTTGGTGCACAGATTCCCCTGGAACAAGTTCCTTGTGTGCAGCAGCAAATAGTTGAGCTATGTAGGCAGCTAAATAAGCCAGTTATTGTTGCCTCACAGCTACTTGAGTCCATGATAGAATATCCACTGCCAACAAGAGCCGAGGTTGCTGATGTGTCCGAAGCAGTAAAACAGCGTGCCGATGCTTTGATGCTTTCTGGTGAGTCAGCTATGGGTCCGCACCCAGAGAAAGCTTTGGCTGTTCTAAGAAGTGTAAGCCTTCGCATTGAGCGGTGGTGGCGTGAAGAGAAGGAATATGAATCTATGGACCTTCCAGCTATTACCTTACGCTATACAGATAGCATCTCCAATGAGATATGTAAATCAGCTGCTAAAATGGCCAACAAGCTGGCTGTGGATGCCATATTTGTCTACACAAAACAAGGACACATGGCTTCCCTTTTGTCAAGTTGTCGTCCAGACTGTCCAATTTTTGCCTTTACTACCTCAACGACTTTAAGGCGACGATTGAATCTTCAATGGGGTCTCATTCCTTTTAGACTGAATTTCTCAGAGGATATGGAGAGTAACCTTTACCGTACCTTTGCTTTGCTGAAAGCAAGAGGTATGGTGAAATCAGGTGACTTGGTAATTGCTGTTTCAGATTTTCTGCAGTCCATTCAGGTAATCAATGTGCCATAATGACATTTTCCGGGTTTCTGATTGTTGCTTCACAATTAACTTGGGCGCTTGTGTTACTAATCAAGAAGCAAGCATTTTCTCTGAAGGAAATGTTGGTGTTTGATCCCAATTTATTCTCCAGAGTGTTAAAGAGCCAGATTAGCTTATTCAAATCAGTTTATTTGCCTGAATTGCAAATAATGATGTAATTAAATCTGCTACATTTGTTTCTTGAGGTATAATATTTTACTTTCAGAAAGTTAAACAAA

Ace file of the UniGene sp_v1.1_unigene27800 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Pyruvate kinase n=2 Tax=Populus trichocarpa RepID=B9HKC4_POPTR	-	-	80%
FL-Next	sp=Pyruvate kinase; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	42%
Blast2go	protein	-	-	88%

Source	ECs	Term	Type	e value	Identity
Blast2go		EC:2.7.1.4-	-	0.0	88%

Source	GOs	Term	Type	Identity
Blast2go	lipid metabolic process	GO:0006629	Biological Process	88%
Blast2go	response to cadmium ion	GO:0046686	Biological Process	88%
Blast2go	pyruvate kinase activity	GO:0004743	Molecular Function	88%
Blast2go	chloroplast stroma	GO:0009570	Cellular Component	88%

Source	InterPros	Term	Type	Identity
Blast2go	Pyruvate kinase	IPR001697	-	88%
Blast2go	Pyruvate kinase-like, insert domain	IPR011037	-	88%
Blast2go	Pyruvate kinase, barrel	IPR015793	-	88%
Blast2go	Pyruvate kinase, alpha/beta	IPR015794	-	88%
Blast2go	Pyruvate kinase, C-terminal	IPR015795	-	88%
Blast2go	Pyruvate kinase, beta-barrel insert domain	IPR015806	-	88%
Blast2go	Pyruvate/Phosphoenolpyruvate kinase	IPR015813	-	88%
Blast2go	Pyruvate kinase, active site	IPR018209	-	88%

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: C0PRS6

Fln msg:

Fln protein: MAYAMHGLLFASFHPRNFSMKPWQSKSMLNRKLLVGNGWLKMQNKRWKGFSVVFEMENSPANVNTESAATKSDAISAIAGDGIRVWSAESSLMEIDTVSEAELKENGFRSTRRTKIVCTIGPASCQFDQLEALAVNGMNVARINMCHGTREWHRRVIRNVRKLNDEKGYAVAIMMDTEGSEIHMGDLGGAPSVKAEDGEVWTFSVRDFKVPLPAHTIHVSYDGFAEDVKVGDELLVDGGMVRFEVVEKLGPDVKCQCTDPGLLLPRANITFWRQGRLVRERNAMLPTISSKDWLDIDFGISEGVDFIAISFVKSAEVIKHLKSYIAARSRDGEIGVIAKIESVDSLKFLEEIIHISDGTMVARGDLGAQIPLEQVPCVQQQIVELCRQLNKPVIVASQLLESMIEYPLPTRAEVADVSEAVKQRADALMLSGESAMGPHPEKALAVLRSVSLRIERWWREEKEYESMDLPAITLRYTDSISNEICKSAAKMANKLAVDAIFVYTKQGHMASLLSSCRPDCPIFAFTTSTTLRRRLNLQWGLIPFRLNFSEDMESNLYRTFALLKARGMVKSGDLVIAVSDFLQSIQVINVP*

Protein Length: 593

Fln nts: CGTCGTGACCTTGGCTGTCATGGGGATTTTACCAAAACACAGCTGAATCCAAATATACGTGGCGGATTAGGGTTTTGAGCATTCGCAAAGGATTTTTATGCTCGTAGGTCTCGAGTTCAGGCTTTTCTTGTTTTATTTACATGCAGTTTGGCACCTTTCTGATAGCATATCACTTCTTGACTAGAGCTCTATATTACTATGCTCAGGTATTTTGGCTGAGAAGTGCGCAGAGATGTTTTGAACGCTAAATGATATACTTCACAAATGAACTCACAGACCCAAATTCAGAACATCAATTCCGCAGAATTTGAGCCTCTGAGAATTGGGCAAATAACATCTGAAAGCCTAAAGAAGTAACTAACATCCTCAGGA_-_ATGGCGTATGCAATGCACGGTTTGCTCTTTGCGTCTTTCCATCCCAGAAACTTCTCCATGAAACCTTGGCAGTCCAAATCCATGCTTAACAGGAAGCTCCTCGTGGGGAATGGCTGGCTGAAAATGCAGAATAAGCGTTGGAAAGGGTTTTCAGTTGTGTTTGAGATGGAGAATTCCCCGGCAAACGTTAATACAGAATCGGCAGCAACAAAATCTGATGCCATTTCAGCCATAGCCGGCGACGGAATCAGGGTTTGGAGCGCCGAGTCGAGCTTGATGGAGATTGATACCGTGAGCGAAGCGGAACTGAAAGAAAATGGTTTTCGTAGCACGCGGAGAACGAAGATTGTCTGTACAATTGGCCCTGCGTCCTGTCAATTTGATCAGCTTGAAGCCCTTGCCGTGAATGGAATGAACGTTGCCAGAATTAATATGTGCCATGGTACGAGAGAATGGCACCGCAGGGTCATTAGGAATGTCAGGAAACTGAATGATGAGAAGGGCTATGCGGTCGCTATTATGATGGATACTGAAGGGAGTGAAATTCACATGGGTGACCTCGGAGGTGCCCCTTCTGTTAAAGCCGAGGATGGTGAGGTTTGGACTTTCAGTGTGCGAGATTTCAAAGTGCCTCTCCCTGCTCATACTATTCATGTCAGCTATGATGGATTTGCAGAGGATGTTAAGGTTGGCGATGAGCTACTTGTGGATGGTGGCATGGTGAGGTTTGAGGTGGTTGAAAAACTTGGACCAGATGTGAAATGTCAGTGTACTGATCCTGGGTTGCTGTTACCACGTGCCAATATAACATTTTGGAGGCAGGGCAGGTTGGTGCGTGAAAGAAATGCCATGCTCCCCACAATTTCCTCCAAGGACTGGCTTGATATAGATTTTGGGATTTCCGAAGGGGTGGATTTCATTGCTATATCTTTTGTGAAATCAGCAGAGGTGATCAAACATTTGAAGAGCTACATTGCAGCTCGATCTCGTGATGGTGAAATTGGTGTCATTGCGAAGATCGAAAGTGTTGATTCTCTAAAGTTTCTTGAAGAGATAATTCACATATCTGATGGAACGATGGTAGCGAGAGGGGACCTTGGTGCACAGATTCCCCTGGAACAAGTTCCTTGTGTGCAGCAGCAAATAGTTGAGCTATGTAGGCAGCTAAATAAGCCAGTTATTGTTGCCTCACAGCTACTTGAGTCCATGATAGAATATCCACTGCCAACAAGAGCCGAGGTTGCTGATGTGTCCGAAGCAGTAAAACAGCGTGCCGATGCTTTGATGCTTTCTGGTGAGTCAGCTATGGGTCCGCACCCAGAGAAAGCTTTGGCTGTTCTAAGAAGTGTAAGCCTTCGCATTGAGCGGTGGTGGCGTGAAGAGAAGGAATATGAATCTATGGACCTTCCAGCTATTACCTTACGCTATACAGATAGCATCTCCAATGAGATATGTAAATCAGCTGCTAAAATGGCCAACAAGCTGGCTGTGGATGCCATATTTGTCTACACAAAACAAGGACACATGGCTTCCCTTTTGTCAAGTTGTCGTCCAGACTGTCCAATTTTTGCCTTTACTACCTCAACGACTTTAAGGCGACGATTGAATCTTCAATGGGGTCTCATTCCTTTTAGACTGAATTTCTCAGAGGATATGGAGAGTAACCTTTACCGTACCTTTGCTTTGCTGAAAGCAAGAGGTATGGTGAAATCAGGTGACTTGGTAATTGCTGTTTCAGATTTTCTGCAGTCCATTCAGGTAATCAATGTGCCATAA___TGACATTTTCCGGGTTTCTGATTGTTGCTTCACAATTAACTTGGGCGCTTGTGTTACTAATCAAGAAGCAAGCATTTTCTCTGAAGGAAATGTTGGTGTTTGATCCCAATTTATTCTCCAGAGTGTTAAAGAGCCAGATTAGCTTATTCAAATCAGTTTATTTGCCTGAATTGCAAATAATGATGTAATTAAATCTGCTACATTTGTTTCTTGAGGTATAATATTTTACTTTCAGAAAGTTAAACAAA

Fln Alignment:

sp_v1.1_unigene27800___RRTKIVCTIGPASCQFDQLEALAVNGMNVARINMCHGTREWHRRVIRNVRKLNDEKG-YAVAIMMDTEGSEIHMGDLGGAPSVKAEDGEVWTFSVRDFKVPLPAHTIHVSYDGFAEDVKVGDELLVDGGMVRFEVVEKLGPDVKCQCTDPGLLLPRANITFWRQGRLVRERNAMLPTISSKDWLDIDFGISEGVDFIAISFVKSAEVIKHLKSYIAARSRDGEIGVIAKIESVDSLKFLEEIIHISDGTMVARGDLGAQIPLEQVPCVQQQIVELCRQLNKPVIVASQLLESMIEYPLPTRAEVADVSEAVKQRADALMLSGESAMGPHPEKALAVLRSVSLRIERWWREEKEYESMDLPAITLRYTDSISNEICKSAAKMANKLAVDAIFVYTKQGHMASLLSSCRPDCPIFAFXXXXXXXXXXXLQWGLIPFRLNFSEDMESNLYRTFALLKARGMVKSGDLVIAVSDFLQSI
C0PRS6_______________RKTKIVCTIGPSTSTPEMIHKLAEEGMNVARLNMSHGDHASHKKVIDLVKEYNAQSADNVIAIMLDTKGPEVRSGDL--PQPIMLGKGQEFIFTIK--RGVGSEKCVSVNYDGFVNDVEKGDTLLVDGGMMSLTVKSKTSDSVICEVIDGGELKSRRHLN-------VRGKSATLPSITDKDWEDIKFGVENEVDFYAVSFVKDAKVVHELKDYL--KSCNSDIQVTVKIESADSIPNLHSIISASDGAMVARGDLGAELPIEEVPLLQEDIIRVCCSMGKPVIVATNMLESMINHPTPTRAEVSDIAIAVREGADAVMLSGETAHGKYPLKAVKVMHTVALRTEATMLDEEP------PACFGRTSKEHMSEMFAFHATMMSNTLGTSIVVFTRTGFMATLLSHYRPFGTIFAFTNEERVKQRLALYRGIRPIYMPFSNDAEETFSKALSLLQKLGMLKEGEQVALVQSGRQPI

SNPs (tot: 50)

Position	A %	C %	G %	T %	Probability
1736		33	66		0.997452
1738		33		66	0.997452
1902	83	16			0.994512
1926	83		16		0.994512
2003		87	12		0.992554
2004	87			12	0.992554
2005	87		12		0.992554
2008	12	87			0.992554
2011		10	90		0.90298
2014	10			90	0.90298
2015			10	90	0.90298
2019			10	90	0.90298
2055		66		33	1.0
2126	10			90	0.90298
2217	81		18		0.999994
2268	77			22	0.999999
2269		77	22		0.999999
2271	77		22		0.999999
2272			77	22	0.999999
2273	77		22		0.999999
2275	77	22			0.999999
2278	11	88			0.923564
2279		88	11		0.923564
2281	22		77		0.999999
2283	77		22		0.999999
2284		66	33		1.0
2285		11		88	0.923564
2287	33		66		1.0
2288	77	22			0.999999
2290	88		11		0.923564
2291		88		11	0.923564
2292		77	22		0.999999
2323	60		40		1.0
2348			40	60	1.0
2349	50		50		1.0
2351	60			40	1.0
2352			50	50	1.0
2353	50			50	1.0
2358		50		50	1.0
2359	50		50		1.0
2360	40			60	1.0
2362			50	50	1.0
2364		50		50	1.0
2365	40			60	1.0
2366		50	50		1.0
2370	50	50			1.0
2371	50		50		1.0
2376		66	33		0.997452
2377	33	66			0.997452
2378	66		33		0.997452

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain