SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene27556

Length: 1144 nt

Origin of reads: Biogeco_1 Biogeco_2 Uagpf Uma

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v1.1_unigene27556 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	hydrolase, hydrolyzing O-glycosyl compounds	-	-	74%
FL-Next	Isoform 3 of Probable galactinol--sucrose galactosyltransferase 2 OS=Arabidopsis thaliana GN=RFS2	-	-	72%
Blast2go	2 (arabidopsis thaliana seed imbibition 2) hydrolyzing o-glycosyl compounds	-	-	81%

Source	InterPros	Term	Type	Identity
Blast2go	Raffinose synthase	IPR008811	-	81%
Blast2go	Aldolase-type TIM barrel	IPR013785	-	81%
Blast2go	Glycoside hydrolase, superfamily	IPR017853	-	81%

Full-Lengther Next Prediction

Fln status: N-terminus

Fln database: sp_plants

Fln subject: Q94A08-3

Fln msg: Unexpected STOP codon at 3' end. Distance to subject end: 295 aas, your sequence is shorter than subject: 268 - 564

Fln protein: MTVTPKISIVDGNLVVQGQVILTGIHDNVILTPGSGTGLVEGAFIGANASESKSHHVFPIGVLEGLRFMCCFRFKLWWMTQRMGTCGRDVPLETQFMLLESKYGSSLSEDMDRGEDEQNIYIVFLPLLEGQFRAVLQGNERNELEVCIESGDSAVTTTQGLHSVYMHAGPDPFEVITDAVKAVERQLQTFHHREKKKLPSFLDWFGWCTWDAFYTDVTAEGVDEGLTSLSEGDTPPRFVIIDDGWQQIGSEVTDPKATVQEGAQFAF*

Protein Length: 269

Fln nts: ACATGGGGATCACTTGGCCAATGCGACAGTCTGCATATCATTCTGTGCGCAAAGGGTGATCGTTTTCTTCTTAGTTGTTATTTTACTTGTGTTTGTGTTGGTTCGATTGGTGATATTCGTGAATTCTAGAGAATTATCTGGAATGTTCTAGTGTTCATTTTACTTCTATTTCTAGGGCTCGTTGAGGTTTGTGAGTTTGTTTTCAGAAGACAAGAATTTTGAGAGTTTTCAGGGTTTCGTCTACTCGGAAGAGTTTCCAACGGTTCTCTGGATTTGCAGTTTAGCTGCGGATTGTAGAGTAGTTAAAGGCAAAGCCAGACAAA_-_ATGACGGTAACTCCGAAGATTTCCATTGTGGACGGCAACCTCGTCGTCCAAGGCCAAGTCATCCTGACGGGGATTCACGATAACGTAATACTTACGCCTGGCTCAGGAACTGGCCTCGTCGAAGGTGCATTCATTGGTGCCAATGCCTCGGAAAGCAAAAGCCACCACGTTTTTCCGATTGGGGTGCTCGAAGGTCTCCGTTTCATGTGTTGCTTCCGCTTCAAGCTGTGGTGGATGACCCAGCGGATGGGAACGTGCGGAAGAGATGTTCCGTTGGAGACGCAATTCATGCTCTTGGAGAGCAAGTACGGGTCTTCTCTCAGCGAAGACATGGATCGTGGGGAAGACGAGCAGAACATTTACATCGTTTTCCTTCCTCTTTTAGAAGGGCAATTCAGAGCCGTTCTTCAAGGCAATGAACGAAATGAATTAGAAGTTTGCATTGAAAGTGGAGATTCTGCGGTGACTACCACCCAAGGGCTGCATTCAGTTTACATGCATGCAGGACCGGATCCGTTTGAAGTTATCACGGACGCTGTCAAGGCTGTGGAAAGACAGTTGCAGACGTTTCACCACCGTGAAAAGAAAAAGTTGCCTTCTTTTCTGGATTGGTTTGGATGGTGCACCTGGGATGCTTTTTACACTGATGTTACTGCTGAAGGCGTGGATGAAGGTTTGACAAGCCTGTCAGAGGGTGATACGCCCCCACGTTTCGTGATCATTGATGATGGTTGGCAACAGATTGGCAGTGAAGTCACTGATCCCAAGGCCACTGTTCAGGAAGGCGCACAGTTTGCTTTTTGATTGACGGGAATTAAAGA

Fln Alignment:

sp_v1.1_unigene27556___MTVTPKISIVDGNLVVQGQVILTGIHDNVILTPGSGTGLVEGAFIGANASESKSHHVFPIGVLEGLRFMCCFRFKLWWMTQRMGTCGRDVPLETQFMLLESKYGSSLSEDMDRGEDEQNIYIVFLPLLEGQFRAVLQGNERNELEVCIESGDSAVTTTQGLHSVYMHAGPDPFEVITDAVKAVERQLQTFHHREKKKLPSFLDWFGWCTWDAFYTDVTAEGVDEGLTSLSEGDTPPRFVIIDDGWQQIGSEVTDPKATVQEGAQFAF*LTGIK
Q94A08-3_____________MTITSNISVQNDNLVVQGKTILTKIPDNIILTPVTGNGFVSGSFIGATFEQSKSLHVFPIGVLEGLRFMCCFRFKLWWMTQRMGSCGKDIPLETQFMLLESK-----DEVEGNGDDAPTVYTVFLPLLEGQFRAVLQGNEKNEIEICFESGDKAVETSQGTHLVYVHAGTNPFEVIRQSVKAVERHMQTFHHREKKKLPSFLDWFGWCTWDAFYTDVTAEGVDEGLKSLSEGGTPPKFLIIDDGWQQIENKEKDENCVVQEGAQFATRLVGIK

SNPs (tot: 19)

Position	A %	C %	G %	T %	Probability
189	71			14	0.984337
195			85	14	0.993533
285	88		11		0.923564
618			85	14	0.999981
619	85		14		0.999981
621	85		14		0.999981
622		85		14	0.999981
626		15	84		0.999986
628		84	15		0.999986
633	14		85		0.999981
636		84	15		0.999986
637	84			15	0.999986
639	15	84			0.999986
641		84	15		0.999986
643	84	15			0.999986
644			15	84	0.999986
979			90	10	0.90298
1111		10	90		0.90298
1114			90	10	0.90298

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain