SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene27520

Length: 1815 nt

Origin of reads: Biogeco_1 Uma

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v1.1_unigene27520 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	long-chain-fatty-acid-CoA ligase (EC:6.2.1.3)	-	-	64%
FL-Next	tr=4-coumarate:CoA ligase; Pinus massoniana.	-	-	27%
Blast2go	acs-like protein	-	-	77%

Source	InterPros	Term	Type	e value	Identity
Blast2go	AMP-dependent synthetase/ligase	IPR000873	-	0.0	77%
Blast2go	AMP-binding, conserved site	IPR020845	-	0.0	77%

Full-Lengther Next Prediction

Fln status: N-terminus

Fln database: coniferopsida.fasta

Fln subject: C1KBY2

Fln msg: Distance to subject end: 72 aas,

Fln protein: MEETQNFVVKIEEGKKAQIGEPSVGPVYRNILTKDGFAPLPQGIESCWDIFSQSAQKYPHNRMLGRRDIENGKAGKYVWLTYQEAYERSIEIGSAIRSSDVGPGGRCGIYGINSSEWIISMEACNSHGIYCVPLYDTLGADAIEFIICHAEVSLVFVQESKIPAVLNCLPRCTGYLKTLVSFGNITDIHKEAADSLGVKAYSWHDFLFLGKKTRCVPSPPKKSDICTIMYTSGTTGEPKGVMLTNENIIAEIAAIDHLLSNTFTEVIETDIYFSFLPLAHVFDRVIEEYCIFKSASIGFWRGDVKLLVEDIMELKPTILCGVPRVFDRIYTGINEKISIGGALKKMLFDFAYSYKLKNMQKGLKQDEAAPLFDKLVFTKVKQGLGGCLRLILSGAAPLARHVEEFLRVTSCSVVVQGYGLTETCAGSFSSLANVFSMVGTVGVPVPCIEVRLKSVPEMGYDALSRTPRGEICIRGNTIFCGYHKREDLTSDVLMDGWFHTGDIGEWLPNGSLKIIDRKKNIFKLSQGEYVAVENLENVYSRCSAIASI

Protein Length: 549

Fln nts: CGTCGTGACCTTGGCTGTCACTCAATCTAGTCCCGCAACGTGTAATCCACCCTCTTTCGGTAGTCTTACTTTGTCCGAAATACGGATAGTGTTCCCGTGGCTCCACAATTTCTAAGCAAAAATTTAATATGTGGCAGACGCGAAGGAGCAGGGAGATCGCTTCTTGAAGC_-_ATGGAGGAGACTCAGAATTTTGTGGTGAAGATCGAAGAGGGAAAGAAAGCGCAGATCGGCGAGCCTTCAGTTGGCCCTGTTTACCGAAACATTTTAACGAAGGATGGCTTCGCACCTCTGCCGCAAGGGATCGAGTCCTGCTGGGACATTTTCAGCCAATCTGCCCAAAAGTATCCGCATAATCGAATGCTTGGTCGACGTGATATTGAAAATGGAAAGGCAGGAAAATATGTATGGTTGACATATCAGGAAGCATATGAAAGATCAATCGAAATTGGATCAGCTATAAGGAGCTCTGATGTTGGTCCAGGAGGCCGTTGTGGTATTTATGGCATTAACTCTTCCGAATGGATTATCAGCATGGAGGCTTGCAACAGCCATGGGATTTATTGTGTTCCGCTCTATGACACTCTTGGTGCGGACGCTATTGAGTTCATTATTTGCCATGCTGAAGTTTCACTGGTTTTTGTACAAGAGAGCAAGATACCTGCAGTGCTGAATTGTTTGCCGAGGTGTACAGGATATTTAAAGACTTTAGTAAGCTTTGGTAACATAACAGATATCCATAAAGAAGCAGCTGATAGTCTTGGAGTGAAAGCATACTCTTGGCATGACTTCTTATTTCTGGGAAAGAAGACAAGATGTGTCCCTTCACCACCCAAGAAGAGTGATATTTGCACAATTATGTATACGAGTGGAACAACAGGAGAACCTAAAGGTGTTATGTTAACGAATGAAAACATCATTGCAGAAATTGCCGCAATTGATCATTTGCTTTCTAATACATTTACAGAGGTGATAGAAACAGACATTTACTTTTCTTTTCTCCCCTTAGCACATGTATTTGATCGAGTTATTGAGGAATATTGCATTTTTAAAAGTGCATCAATAGGCTTCTGGCGTGGGGATGTGAAGCTTTTGGTGGAAGATATTATGGAGTTAAAGCCAACTATTTTATGTGGTGTCCCTCGTGTGTTCGATCGTATATACACAGGTATAAATGAGAAGATATCAATTGGCGGAGCATTAAAGAAAATGCTGTTTGATTTTGCATACAGCTACAAGTTAAAGAACATGCAGAAGGGTTTGAAGCAGGATGAAGCAGCACCACTTTTTGATAAGCTTGTTTTTACTAAGGTAAAACAAGGTCTAGGAGGTTGTCTTCGTCTGATCCTTTCTGGAGCAGCCCCACTGGCTAGACACGTAGAAGAGTTTTTGAGAGTTACGAGCTGTAGTGTTGTAGTACAAGGATATGGTTTGACTGAAACATGTGCTGGAAGTTTTAGTTCCTTGGCCAATGTCTTCTCCATGGTTGGAACAGTTGGAGTGCCTGTTCCATGCATTGAAGTGCGGCTTAAGTCGGTCCCTGAGATGGGCTATGATGCACTTTCCAGAACACCACGTGGAGAGATTTGTATAAGGGGAAACACCATATTTTGTGGGTATCACAAGCGTGAAGATCTGACTAGTGATGTTCTTATGGATGGTTGGTTTCACACAGGTGATATTGGCGAGTGGCTACCAAATGGATCATTGAAAATCATTGATAGGAAAAAAAATATTTTCAAACTTTCACAGGGTGAATACGTTGCAGTGGAAAATTTAGAGAATGTATACAGCCGTTGTTCTGCTATTGCTTCGATAT

Fln Alignment:

sp_v1.1_unigene27520___KTRCVPSPPKKSDICTIMYTSGTTGEPKGVMLTXXXXXXXXXXI-DHLLSNTFTEVIETDIYFSFLPLAHVFDRVIEEYCIFKSASIGFWRGDVKLLVEDIMELKPTILCGVPRVFDRIYTGINEKISIGGALKKMLFDFAYSYKLKNMQKGLKQDEAAPLFDKLVFTKVKQGLGG-----CLRLILSGAAPLARHVEEFLRVTSCSVVV-QGYGLTET---CAGSFSSLANVFSMV-GTVGVPVPCIEVRLKSVPEMGYDALSRTPRGEICIRGNTIFCGY-HKREDLTSDVLMDGWFHTGDIGEWLPNGSLKIIDRKKNIFKLSQGEYVAVENLE
C1KBY2_______________ETQCPAVKIHPDDVVALPYSSGTTGLPKGVMLTHKGLVSSVAQQVDGENPNLYFH--SEDVILCVLPLFHIYS------------------------------LNSVLLCALR----------------AGAATLIMQKFNLTTCLELIQK--YKVTVAPIVPPIVLDITKSPIVSQYDVSSVRIIMSGAAPLGKELEDALRERFPKAIFGQGYGMTEAGPVLAMNLAFAKNPFPVKSGSCGTVVRNAQIKILDT-ETG-ESLPHNQAGEICIRGPEIMKGYINDPESTAATIDEEGWLHTGDVGYIDDDEEIFIVDRVKEIIKY-KGFQVAPAELE

SNPs (tot: 23)

Position	A %	C %	G %	T %	Probability
653		12		87	0.992554
654		12	87		0.992554
655		12		87	0.992554
656	12		87		0.992554
1085	80			20	0.995493
1172	20			80	0.995493
1468		25		75	0.996473
1496	50		25		0.995533
1499			25	75	0.996473
1510		25	75		0.996473
1523		25	75		0.996473
1533		25		75	0.996473
1539			75	25	0.996473
1548		75		25	0.996473
1550		25		75	0.996473
1554	75			25	0.996473
1567	66		33		0.997452
1575	66		33		0.997452
1584		66	33		0.997452
1586		66		33	0.997452
1591	66			33	0.997452
1597			66	33	0.997452
1601		33		66	0.997452

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain