SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene27440

Length: 2877 nt

Origin of reads: Biogeco_1 Biogeco_2 Uagpf Uma

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>sp_v1.1_unigene27440
CGTCGTGACCTTGGCTGTCACTCAATTTTTTCTATGTAAGCGAAAGCCCGCGCACAGTGTCAAATCCGCTTTGAACCCTGCGGAACGTCAAATACGCTTTTGCTTTGGCAGCATACAGAGGGAAAACATGGGCGACGACGGCGCTGCTTCTCCCTTATCGCCCCAGCTAGCCTTGAAAGGCAATGCCTACCTGCAAGTCGTCATCCCAAAGCGCATACAAATGTTCGAGCGAATTCAGGCAGAGCAACAGCTTCACCGTCTACAGATCGGAGGAGAACCTATAAGGATTACTCTTCCAGATGGTCAAGTCAAGGAGGGGAAAAGATGGATTACTTCACCTATGGATATTGCTAAGGAAATTTCAAAAGGATTGGCTGCATCAGCGTTAATATCAGAGGTGAATGGAACTCTTTGGGACCTGTTGAGGCCTCTAGAGGGAGACTGTCAATTGAAATTGTTCACATTTGACACCAATGAAGGACGTGATACATTTTGGCATTCTAGTGCTCACATCCTTGGGGAGTCTCTTGAAATGGAGTATGGCTGCAAACTATGTATTGGACCCTGTACAACTCGAGGAGAGGGATTTTATTACGACGCCTTCTATGACGATGTTACCTTGAATGATGACGAGTTCAAAAAGATTCATGCTCAGGCTACAAAAGCTGCTATGGAAAAACAACCATTTGAACGGATAGAAGTATCAAGGCAGCAGGCCCTTGAAATGTTTGCTGATAATCCTTTCAAGGTTGAAATTATAAAGGAACTGCCTGAGGATAAAACAATAACAGTGTACAGATGTGGACCTCTGGTAGATCTTTGTCGTGGGCCACACATCCCCAATACGTCTCATGTGAAAGCCTTTGCTTGTCTCAAGGCTTCAGCATCTTATTGGAGAGGCAAATCAGATCGTGAAAGTCTCCAAAGGGTCTACGGAATTTCATACCCAGATTCCAAAAAGCTGAAAGAATATCTTACGCAGTTAGAAGAAGCTAAGAAACGTGATCACAGGGTTGTGGGGCTGAATCAAGAACTTTTCTTTTTCCATCCACTTAGCCCTGGTAGTTGCTTCTTTTTACCTCATGGTACAAGAATCTACAATACATTGATGAATTTTATACGCAATGAATACAGGAAGAGGGGTTACCATGAGGTGATAACTCCAAACATGTTCAATATGCAACTCTGGGAAAAATCGGGGCATGCAGCTAATTACAAAGAAAACATGTTTGTGTTTGAGGTTGAGAAACAGGAATTTGGGCTTAAGCCTATGAATTGTCCAGGGCACTGTTTGATGTTTGAACATAGAGTGCGATCATACAGAGAATTACCATTGCGTTTGGCTGATTTTGGGGTTTTGCATCGCAATGAACTTAGTGGTGCGTTGACTGGGCTGACTCGTGTTCGGCGATTTCAACAGGATGATGCACATATCTTTTGCAAGGAATCTCAGATCAAAGATGAGGTTGGAGGTGTGCTGGATTTCTTACAATATGCATATGAGATATTCGGCTTTACATTTGAACTAGAGTTGTCAACTATGCCTGAAAAACACTTGGGAGATCTAGAAACGTGGCAAAGAGCTGAGGCAGCATTAGCTGAAGCCTTGAATGCATTTGGAAAGCCATGGCAGATTAATGAAGGTGATGGTGCTTTTTATGGGCCCAAGATTGACATCACCGTTTTTGATGCTTTGAAAAGAAAATTCCAGTGTGCGACAATTCAGCTTGATTTTCAGCTTCCTATTCGATTTGATTTATCTTACTCAGCTGAGGATGAAGCAAAGAGGGAAAGGCCTGTCATGATACACCGGGCCATACTTGGTTCTGTTGAGCGCATGCTTGCAATACTCTTGGAGCATTATGCAGGGAAATGGCCTTTCTGGCTCAGTCCTCGGCAGGCAATTGTATGCCCGGTGTCAGAGAAATCTTCAGAGTATGCATTGAGAGTTCGTGATCAGATTCATCAAGCTGGTTATTATGTTGATGTTGATACGTCAGACAGAAAGATTCAGAAGAAGGTCCGGGAGGCACAGCTAGCACAATACAACTATATTTTAGTTGTTGGTGAAGAGGAAGCAAACACTGGACAGGTAAGTGTTCGCATCCGAGATAAATCAACACATGAAGTAAGGAGTATGGATGAACTTCTTAAATTGTTTAGAGAGGAGATTGAAGCGTTTCATTAGCTGAAGGCAGGGTAAGCTGCTTAGAAAATTATAAAATTTCAGGGTTAAAATTCTCCTCGGTCTTTATTTCTCAGCTAATTTTGTTGCCCTGAATTGGAAAGAAATTTGATATCATATTTGGAAGTGAAGAAATGCACAATGTAGTGTAAAAACCTGTGTTCTCTGATCAGGAAATGTATATCAAGGTTCATGATTTGACCTGAACCAAGTCAAAGTTCTTATTGATGAAATTTTGTGTATTGATGAGTAAAGTGACCACAGAAATATAAAAAATACCTTCGATATCACTCCTTGAATTGGCTAATAAGGGACGCCATTCTTGTTTAGTCTCTGATATTTTTCTTTCTAGTAGAGGACCCCCAAAATCATTGATTTTCAAATTCGGCTGTATGTAATATGAAACTGTGAGATTTCTGACAATGCTATCCTTGATTTTCTGAATTTTCCGTTACTGTAATAATGTTTTTGCAGTTTAAATCGACCAGCATTACACATCTGGGCCTTGGAAATTCTTCAAAACGCAAGTTTTTAAAAATTATATATGAAACGTTTTTGACACTTGAAGCGCCAATAATCATATCGGCATTCGTTAGAGAATTGCGAATGAAAAATTCCATCGTTTAAACTAAACTTTGAATCCGAAGAATGCTAGTGCTGGCTGTAAATTTGTCCCTAAATATTATTGGAAA

Ace file of the UniGene sp_v1.1_unigene27440 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	threonine-tRNA ligase (EC:6.1.1.3); K01868 threonyl-tRNA synthetase [EC:6.1.1.3]	-	-	77%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	97%
Blast2go	threonyl-trna synthetase	-	-	86%

Source	ECs	Term	Type	e value	Identity
AutoFact	Threonine--tRNA ligase.	EC:6.1.1.3	-	0.0	77%

Source	KEGGs	Term	Type	e value	Identity
AutoFact	Aminoacyl-tRNA biosynthesis	00970		0.0	77%

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: D5ACE7

Fln msg: your sequence is longer than subject: 687 - 215

Fln protein: MGDDGAASPLSPQLALKGNAYLQVVIPKRIQMFERIQAEQQLHRLQIGGEPIRITLPDGQVKEGKRWITSPMDIAKEISKGLAASALISEVNGTLWDLLRPLEGDCQLKLFTFDTNEGRDTFWHSSAHILGESLEMEYGCKLCIGPCTTRGEGFYYDAFYDDVTLNDDEFKKIHAQATKAAMEKQPFERIEVSRQQALEMFADNPFKVEIIKELPEDKTITVYRCGPLVDLCRGPHIPNTSHVKAFACLKASASYWRGKSDRESLQRVYGISYPDSKKLKEYLTQLEEAKKRDHRVVGLNQELFFFHPLSPGSCFFLPHGTRIYNTLMNFIRNEYRKRGYHEVITPNMFNMQLWEKSGHAANYKENMFVFEVEKQEFGLKPMNCPGHCLMFEHRVRSYRELPLRLADFGVLHRNELSGALTGLTRVRRFQQDDAHIFCKESQIKDEVGGVLDFLQYAYEIFGFTFELELSTMPEKHLGDLETWQRAEAALAEALNAFGKPWQINEGDGAFYGPKIDITVFDALKRKFQCATIQLDFQLPIRFDLSYSAEDEAKRERPVMIHRAILGSVERMLAILLEHYAGKWPFWLSPRQAIVCPVSEKSSEYALRVRDQIHQAGYYVDVDTSDRKIQKKVREAQLAQYNYILVVGEEEANTGQVSVRIRDKSTHEVRSMDELLKLFREEIEAFH*

Protein Length: 688

Fln nts: CGTCGTGACCTTGGCTGTCACTCAATTTTTTCTATGTAAGCGAAAGCCCGCGCACAGTGTCAAATCCGCTTTGAACCCTGCGGAACGTCAAATACGCTTTTGCTTTGGCAGCATACAGAGGGAAAAC_-_ATGGGCGACGACGGCGCTGCTTCTCCCTTATCGCCCCAGCTAGCCTTGAAAGGCAATGCCTACCTGCAAGTCGTCATCCCAAAGCGCATACAAATGTTCGAGCGAATTCAGGCAGAGCAACAGCTTCACCGTCTACAGATCGGAGGAGAACCTATAAGGATTACTCTTCCAGATGGTCAAGTCAAGGAGGGGAAAAGATGGATTACTTCACCTATGGATATTGCTAAGGAAATTTCAAAAGGATTGGCTGCATCAGCGTTAATATCAGAGGTGAATGGAACTCTTTGGGACCTGTTGAGGCCTCTAGAGGGAGACTGTCAATTGAAATTGTTCACATTTGACACCAATGAAGGACGTGATACATTTTGGCATTCTAGTGCTCACATCCTTGGGGAGTCTCTTGAAATGGAGTATGGCTGCAAACTATGTATTGGACCCTGTACAACTCGAGGAGAGGGATTTTATTACGACGCCTTCTATGACGATGTTACCTTGAATGATGACGAGTTCAAAAAGATTCATGCTCAGGCTACAAAAGCTGCTATGGAAAAACAACCATTTGAACGGATAGAAGTATCAAGGCAGCAGGCCCTTGAAATGTTTGCTGATAATCCTTTCAAGGTTGAAATTATAAAGGAACTGCCTGAGGATAAAACAATAACAGTGTACAGATGTGGACCTCTGGTAGATCTTTGTCGTGGGCCACACATCCCCAATACGTCTCATGTGAAAGCCTTTGCTTGTCTCAAGGCTTCAGCATCTTATTGGAGAGGCAAATCAGATCGTGAAAGTCTCCAAAGGGTCTACGGAATTTCATACCCAGATTCCAAAAAGCTGAAAGAATATCTTACGCAGTTAGAAGAAGCTAAGAAACGTGATCACAGGGTTGTGGGGCTGAATCAAGAACTTTTCTTTTTCCATCCACTTAGCCCTGGTAGTTGCTTCTTTTTACCTCATGGTACAAGAATCTACAATACATTGATGAATTTTATACGCAATGAATACAGGAAGAGGGGTTACCATGAGGTGATAACTCCAAACATGTTCAATATGCAACTCTGGGAAAAATCGGGGCATGCAGCTAATTACAAAGAAAACATGTTTGTGTTTGAGGTTGAGAAACAGGAATTTGGGCTTAAGCCTATGAATTGTCCAGGGCACTGTTTGATGTTTGAACATAGAGTGCGATCATACAGAGAATTACCATTGCGTTTGGCTGATTTTGGGGTTTTGCATCGCAATGAACTTAGTGGTGCGTTGACTGGGCTGACTCGTGTTCGGCGATTTCAACAGGATGATGCACATATCTTTTGCAAGGAATCTCAGATCAAAGATGAGGTTGGAGGTGTGCTGGATTTCTTACAATATGCATATGAGATATTCGGCTTTACATTTGAACTAGAGTTGTCAACTATGCCTGAAAAACACTTGGGAGATCTAGAAACGTGGCAAAGAGCTGAGGCAGCATTAGCTGAAGCCTTGAATGCATTTGGAAAGCCATGGCAGATTAATGAAGGTGATGGTGCTTTTTATGGGCCCAAGATTGACATCACCGTTTTTGATGCTTTGAAAAGAAAATTCCAGTGTGCGACAATTCAGCTTGATTTTCAGCTTCCTATTCGATTTGATTTATCTTACTCAGCTGAGGATGAAGCAAAGAGGGAAAGGCCTGTCATGATACACCGGGCCATACTTGGTTCTGTTGAGCGCATGCTTGCAATACTCTTGGAGCATTATGCAGGGAAATGGCCTTTCTGGCTCAGTCCTCGGCAGGCAATTGTATGCCCGGTGTCAGAGAAATCTTCAGAGTATGCATTGAGAGTTCGTGATCAGATTCATCAAGCTGGTTATTATGTTGATGTTGATACGTCAGACAGAAAGATTCAGAAGAAGGTCCGGGAGGCACAGCTAGCACAATACAACTATATTTTAGTTGTTGGTGAAGAGGAAGCAAACACTGGACAGGTAAGTGTTCGCATCCGAGATAAATCAACACATGAAGTAAGGAGTATGGATGAACTTCTTAAATTGTTTAGAGAGGAGATTGAAGCGTTTCATTAG___CTGAAGGCAGGGTAAGCTGCTTAGAAAATTATAAAATTTCAGGGTTAAAATTCTCCTCGGTCTTTATTTCTCAGCTAATTTTGTTGCCCTGAATTGGAAAGAAATTTGATATCATATTTGGAAGTGAAGAAATGCACAATGTAGTGTAAAAACCTGTGTTCTCTGATCAGGAAATGTATATCAAGGTTCATGATTTGACCTGAACCAAGTCAAAGTTCTTATTGATGAAATTTTGTGTATTGATGAGTAAAGTGACCACAGAAATATAAAAAATACCTTCGATATCACTCCTTGAATTGGCTAATAAGGGACGCCATTCTTGTTTAGTCTCTGATATTTTTCTTTCTAGTAGAGGACCCCCAAAATCATTGATTTTCAAATTCGGCTGTATGTAATATGAAACTGTGAGATTTCTGACAATGCTATCCTTGATTTTCTGAATTTTCCGTTACTGTAATAATGTTTTTGCAGTTTAAATCGACCAGCATTACACATCTGGGCCTTGGAAATTCTTCAAAACGCAAGTTTTTAAAAATTATATATGAAACGTTTTTGACACTTGAAGCGCCAATAATCATATCGGCATTCGTTAGAGAATTGCGAATGAAAAATTCCATCGTTTAAACTAAACTTTGAATCCGAAGAATGCTAGTGCTGGCTGTAAATTTGTCCCTAAATATTATTGGAAA

Fln Alignment:

sp_v1.1_unigene27440___MPEKHLGDLETWQRXXXXXXXXXXAFGKPWQINEGDGAFYGPKIDITVFDALKRKFQCATIQLDFQLPIRFDLSYSAEDEAKRERPVMIHRAILGSVERMLAILLEHYAGKWPFWLSPRQAIVCPVSEKSSEYALRVRDQIHQAGYYVDVDTSDRKIQKKVREAQLAQYNYILVVGEEEANTGQVSVRIRDKSTHEVRSMDELLKLFREEIEAFH
D5ACE7_______________MPEKHLGDLETWQRAEAALTEALNAFGKPWQINEGDGAFYGPKIDITVFDALKRKFQCATIQLDFQLPIRFDLSYSTEDEAKRERPVMIHRAILGSVERMLAILLEHYAGKWPFWLSPRQAIVCPVSEKSSDYALRVRDQIHQAGYYVDVDTSDRKIQKKIREAQLAQYNYILVVGEEEANTGQVSVRIRDKSAHEVRSMDELLKLFREEIEAFH

SNPs (tot: 144)

Position	A %	C %	G %	T %	Probability
307	40		60		1.0
342		60		40	1.0
433		42	57		1.0
586	60	40			1.0
668		25		75	0.996473
701	90		9		0.999872
706			9	85	0.999726
711			14	85	1.0
712		90	9		0.999872
715	14	85			1.0
716		9	4	85	0.999726
717		85	14		1.0
718	9			90	0.999872
729			11	88	0.999952
731	88			11	0.999952
736		11		88	0.999952
737	5	11		82	0.999919
738	11	82			0.999919
740			17	82	1.0
741		70	17	11	1.0
742	17	82			1.0
743	82		17		1.0
744	5	11		82	0.999919
747	18		12	68	1.0
748	6	73	20		1.0
750		6		73	1.0
751		20	20	60	1.0
753	20	73	6		1.0
754	6		26	66	1.0
758	7	21		64	1.0
760		28		64	1.0
762	9			63	1.0
764		10	90		0.90298
765	90		10		0.90298
767		20	10	70	0.999998
768	30			70	1.0
769	70	20	10		0.999998
770		10	20	70	0.999998
772	22			77	0.999999
773	11	88			0.923564
776	87			12	0.992554
777		12		87	0.992554
778	12		87		0.992554
791		16		83	0.994512
796	66	33			1.0
797	33		66		1.0
1332		11		88	0.923564
1523	88	11			0.923564
1524	88	11			0.923564
1526	88			11	0.923564
1583	90		10		0.90298
1586	90		10		0.90298
1631		10		90	0.90298
1936	25		75		1.0
1963	80			20	0.999996
1967	20			80	0.999996
1968			20	80	0.999996
1969		20		80	0.999996
1972	18	81			0.999994
1974	81		18		0.999994
1978		18		81	0.999994
1981		18		81	0.999994
1982			20	80	0.999996
2038		33		66	1.0
2053		11		88	0.923564
2072	87	12			0.992554
2076			87	12	0.992554
2116		85	14		0.993533
2126	85	14			0.993533
2127		14		85	0.993533
2128	85	14			0.993533
2167	16		83		0.994512
2175		16	83		0.994512
2312	27		72		1.0
2375	10			90	0.90298
2377	90			10	0.90298
2387			10	90	0.90298
2389		88		11	0.923564
2392	88	11			0.923564
2393	88		11		0.923564
2395		11		88	0.923564
2515		40		60	1.0
2518	75			25	0.996473
2520			75	25	0.996473
2522	75		25		0.996473
2523	25	75			0.996473
2524	58			41	1.0
2525	58	41			1.0
2527			41	58	1.0
2528		41		58	1.0
2529		58	41		1.0
2530		58		41	1.0
2532			41	58	1.0
2534			58	41	1.0
2535	41	58			1.0
2536	58	41			1.0
2537	58	41			1.0
2538	45			54	1.0
2539	54	45			1.0
2541		54	45		1.0
2543	54			45	1.0
2545	54	45			1.0
2547		45	54		1.0
2548	45			54	1.0
2549			54	45	1.0
2551	54		45		1.0
2552	45		54		1.0
2553			45	54	1.0
2555	54	45			1.0
2557		54	45		1.0
2558	45	54			1.0
2559	54		45		1.0
2562			57	42	1.0
2654		89	3	7	0.998606
2656	7	89	3		0.998606
2657			7	92	0.999532
2658		89		7	0.998606
2663	89	10			1.0
2664	10			89	1.0
2665	10			89	1.0
2666			10	85	1.0
2670		10		89	1.0
2735			92	8	0.999718
2736	92		8		0.999718
2739		92		8	0.999718
2740	92		8		0.999718
2741	8		92		0.999718
2746	8		91		0.999766
2747	8			91	0.999766
2748		91	8		0.999766
2752	8		91		0.999766
2754		91	8		0.999766
2757		8		91	0.999807
2813	90		10		0.999898
2855	77			5	1.0
2857	85			14	0.993533
2858	85		14		0.993533
2860	42			57	1.0
2864			85	14	0.993533
2866			85	14	0.993533
2872		85		14	0.993533
2873	85		14		0.993533
2874	85	14			0.993533
2876			85	14	0.993533

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain