SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene26574

Length: 2122 nt

Origin of reads: Biogeco_1 Biogeco_2 Uagpf Uma

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>sp_v1.1_unigene26574
CGTCGTGACCTTGGCTGTCACTCAATGATTCCCTTGGTGATTAAACTCTGTAGCGTCGGACGTCGTGACCTTGGCTGTCACTCATTTTACTTACTATTACTTCGGCCTCGATCTCATAGCTGAGGTTACTTTGCCCAGATCTGATAGCTAAGGCACGTACCATTACTTCACCCCGGATCTCATGGCTGAGGTTACTTCGCCCAGATCTCATAGCTGAGGCACAACAAAATTAGGTTGGGGTGGATCAGGTACTTATTAGATTTCATCATTTCTGCAATTCTTAACACAGAATTGCTCTATTTCTGCAATATTTACAACTTTCTCAATAAAGAACTAGATTTCCCAACTAGTGGTATCGAACTGGCAGATGCAGGAATGAATCTCCACACCTCAATCTCTCCTGCCTGCAGCTCCAATTCGATATTATTCGTTTGACCGTTGATATGGTGGTTTGCTTCTCACATGGGATGCTGGGTTTTTCATTATTCCTTTTCAAAGTGCACTTTCTGTAAATTAGCTATTCGTGTTTTCTATGACTGGTATGACAATTCTCTTTCATGGCCAGAACTCACAAGGCCTTTATTGCTCTGGCCAGGTAATTGGTTGCATTTGGTATTCTTTATCGTTTCCCTGTAATTATACTCTTATGTTTTTCTAGAAACCCTCGGTTTCATTGACAGAGCCTTCGTATGCTTTGTTTTGTTTAGTTAAATCATCAGTGACTGGTGTCAAATCATCAATGACTGGTGACATCTATTTTCGTAACCCCTGCCACATACAACTACTCAGTCATGTAATTTCTTTCTAGGTTCCTCTTTCTCCTTTGAACTGCTCGAAGAATTTAATGGGTGTACTCAGCATTTGGGTTATCGTGTGCTTTTCTTAATTGTATTCATGGTTGAATGGAGTTCGTATCAGGGTTTTTCTTGTTGTGGGGAGATTCCTGAAATTTGTATCGGTTCTTGTCTGAATTAGTGTTGTGGAGCGAAAGAGGGTTTTTGTCTGAAATGTTGTTGTGGAGAGAAATATGGTATATAGAGGCAAAGGCAAACTTTCTGTTTCAGTGGGAAGACGAGTTCAAGGGAGAGAAAAACGAGGGTGGGTTTGAAGCTACCAATCCCAGCTTTGGAAGACGCATTTTCTCAGCCAATGCAATTGCCACTTCCACTGCAGAAGCCCACCAACATGAATGGTGCTCGCAAGTTACCCTGTATTCCTCTTGAAGAAGTTACACTAGAATATCTCCAGAAGATTTCAACTTTGGGGTGTGGAAGCAGTGGCAAGGTCTACATGGTTAAGCATGTCAAGACTAGGAAAATTTATGCTTTGAGGAAAAGCACGAGCTTGCTGTTCGAAATAAAATAATGAGGGAAATGGAGATTCTCCTATGGGCGAATTCTCCACACATCTTGCAGTGTTATGGCATAGTCGAGCGAATTCTCCCCACATCGTGCAGTGTTATGGCATATTCGATCGAAGAGGAGAGATCTCTTTTGTGTTGGACTACATGGAAGGTGGAACCCTTGCGCAGGTTCTTCAAGCCCACAAGAAAATCCCAGAACACTATTTGGCTGAGGTTGCCAGACAGGTGCTGAAGGGCTTGCATTACCTGCACCAGAACAAAATTGTTCACCGTGATATAAAGCCCTCCAATTTGCTGATCAACAAGAGAAGAGAAGTGAAGATTGCCGATTTCGGTGTCAGCACTGTGTTGGCTCACACTTTGGCCCAGTGTAATTCCTTCGTGGGCACTTGTGCTTATATGAGTCCTGAAAGGTTCGATCCTGATGGGTACGGTGGAAAGTATGATGGATGCTCTGCAGATATATGGAGCTTGGGATTATCTTTGTTGGAATGTGCGCTTGGAAGGTTCCCTTGTTTGTCTCCGGGGCAGAGGCCTGATTGGCCTACTTTAATGGTGGCCATCTGTTTGGGCGACCCTCCATCCCCGCCACCTGATGCATCGCCAGAGTTTCAGAGTTTTATCAGATGTTGCCTTCAAAAGGATGCATTACTTCGCCATACTGCACATCGGCTGCTTTCGCATCCTTTTTTGAAGAAGTATGAACAGCAATCTTGTGACCTGGCTCCCCTCCTGCAGTCTTTACACCTGTAGAATTTT

Ace file of the UniGene sp_v1.1_unigene26574 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	MKK9; MKK9 (MAP KINASE KINASE 9); MAP kinase kinase/ kinase/ protein kinase activator	-	-	54%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	83%
Blast2go	protein	-	-	77%

Source	GOs	Term	Type	Identity
Blast2go	response to wounding	GO:0009611	Biological Process	77%
Blast2go	positive regulation of cellular transcription, DNA-dependent	GO:0045941	Biological Process	77%
Blast2go	protein autophosphorylation	GO:0046777	Biological Process	77%
Blast2go	ethylene biosynthetic process	GO:0009693	Biological Process	77%
Blast2go	ethylene mediated signaling pathway	GO:0009873	Biological Process	77%
Blast2go	camalexin biosynthetic process	GO:0010120	Biological Process	77%
Blast2go	response to salt stress	GO:0009651	Biological Process	77%
Blast2go	MAP kinase kinase activity	GO:0004708	Molecular Function	77%
Blast2go	protein binding	GO:0005515	Molecular Function	77%
Blast2go	protein kinase activator activity	GO:0030295	Molecular Function	77%

Source	InterPros	Term	Type	Identity
Blast2go	Protein kinase, catalytic domain	IPR000719	-	77%
Blast2go	Serine/threonine- / dual-specificity protein kinase, catalytic domain	IPR002290	-	77%
Blast2go	Serine/threonine-protein kinase, active site	IPR008271	-	77%
Blast2go	Protein kinase-like domain	IPR011009	-	77%
Blast2go	Serine/threonine-protein kinase-like domain	IPR017442	-	77%
Blast2go	Tyrosine-protein kinase, catalytic domain	IPR020635	-	77%

Full-Lengther Next Prediction

Fln status: Putative Complete

Fln database: coniferopsida.fasta

Fln subject: D5AD68

Fln msg: Warning!, your query overlaps and the subject is separated, and overlapping frame ERROR between 1436 and 1430, W1: There is no M at the beginning,

Fln protein: RQRQTFCFSGKTSSRERKTRVGLKLPIPALEDAFSQPMQLPLPLQKPTNMNGARKLPCIPLEEVTLEYLQKISTLGCGSSGKVYMVKHVKTRKIYAxxxKHELAVRNKIMREMEILLWANSPHILQCYGxxxxxSPHIVQCYGIFDRRGEISFVLDYMEGGTLAQVLQAHKKIPEHYLAEVARQVLKGLHYLHQNKIVHRDIKPSNLLINKRREVKIADFGVSTVLAHTLAQCNSFVGTCAYMSPERFDPDGYGGKYDGCSADIWSLGLSLLECALGRFPCLSPGQRPDWPTLMVAICLGDPPSPPPDASPEFQSFIRCCLQKDALLRHTAHRLLSHPFLKKYEQQSCDLAPLLQSLHL*

Protein Length: 361

Fln nts: CGTCGTGACCTTGGCTGTCACTCAATGATTCCCTTGGTGATTAAACTCTGTAGCGTCGGACGTCGTGACCTTGGCTGTCACTCATTTTACTTACTATTACTTCGGCCTCGATCTCATAGCTGAGGTTACTTTGCCCAGATCTGATAGCTAAGGCACGTACCATTACTTCACCCCGGATCTCATGGCTGAGGTTACTTCGCCCAGATCTCATAGCTGAGGCACAACAAAATTAGGTTGGGGTGGATCAGGTACTTATTAGATTTCATCATTTCTGCAATTCTTAACACAGAATTGCTCTATTTCTGCAATATTTACAACTTTCTCAATAAAGAACTAGATTTCCCAACTAGTGGTATCGAACTGGCAGATGCAGGAATGAATCTCCACACCTCAATCTCTCCTGCCTGCAGCTCCAATTCGATATTATTCGTTTGACCGTTGATATGGTGGTTTGCTTCTCACATGGGATGCTGGGTTTTTCATTATTCCTTTTCAAAGTGCACTTTCTGTAAATTAGCTATTCGTGTTTTCTATGACTGGTATGACAATTCTCTTTCATGGCCAGAACTCACAAGGCCTTTATTGCTCTGGCCAGGTAATTGGTTGCATTTGGTATTCTTTATCGTTTCCCTGTAATTATACTCTTATGTTTTTCTAGAAACCCTCGGTTTCATTGACAGAGCCTTCGTATGCTTTGTTTTGTTTAGTTAAATCATCAGTGACTGGTGTCAAATCATCAATGACTGGTGACATCTATTTTCGTAACCCCTGCCACATACAACTACTCAGTCATGTAATTTCTTTCTAGGTTCCTCTTTCTCCTTTGAACTGCTCGAAGAATTTAATGGGTGTACTCAGCATTTGGGTTATCGTGTGCTTTTCTTAATTGTATTCATGGTTGAATGGAGTTCGTATCAGGGTTTTTCTTGTTGTGGGGAGATTCCTGAAATTTGTATCGGTTCTTGTCTGAATTAGTGTTGTGGAGCGAAAGAGGGTTTTTGTCTGAAATGTTGTTGTGGAGAGAAATATGGTATATAGAGGCAAAGGCAAACTTTCTGTTTCAGTGGGAAGACGAGTTCAAGGGAGAGAAAAACGAGGGTGGGTTTGAAGCTACCAATCCCAGCTTTGGAAGACGCATTTTCTCAGCCAATGCAATTGCCACTTCCACTGCAGAAGCCCACCAACATGAATGGTGCTCGCAAGTTACCCTGTATTCCTCTTGAAGAAGTTACACTAGAATATCTCCAGAAGATTTCAACTTTGGGGTGTGGAAGCAGTGGCAAGGTCTACATGGTTAAGCATGTCAAGACTAGGAAAATTTATGCTxxxxxxxxxAAGCACGAGCTTGCTGTTCGAAATAAAATAATGAGGGAAATGGAGATTCTCCTATGGGCGAATTCTCCACACATCTTGCAGTGTTATGGCATxxxxxxxxxxxxTTCTCCCCACATCGTGCAGTGTTATGGCATATTCGATCGAAGAGGAGAGATCTCTTTTGTGTTGGACTACATGGAAGGTGGAACCCTTGCGCAGGTTCTTCAAGCCCACAAGAAAATCCCAGAACACTATTTGGCTGAGGTTGCCAGACAGGTGCTGAAGGGCTTGCATTACCTGCACCAGAACAAAATTGTTCACCGTGATATAAAGCCCTCCAATTTGCTGATCAACAAGAGAAGAGAAGTGAAGATTGCCGATTTCGGTGTCAGCACTGTGTTGGCTCACACTTTGGCCCAGTGTAATTCCTTCGTGGGCACTTGTGCTTATATGAGTCCTGAAAGGTTCGATCCTGATGGGTACGGTGGAAAGTATGATGGATGCTCTGCAGATATATGGAGCTTGGGATTATCTTTGTTGGAATGTGCGCTTGGAAGGTTCCCTTGTTTGTCTCCGGGGCAGAGGCCTGATTGGCCTACTTTAATGGTGGCCATCTGTTTGGGCGACCCTCCATCCCCGCCACCTGATGCATCGCCAGAGTTTCAGAGTTTTATCAGATGTTGCCTTCAAAAGGATGCATTACTTCGCCATACTGCACATCGGCTGCTTTCGCATCCTTTTTTGAAGAAGTATGAACAGCAATCTTGTGACCTGGCTCCCCTCCTGCAGTCTTTACACCTGTAGAATTTT

Fln Alignment:

sp_v1.1_unigene26574___SSRERKTRVGLKLPIPALEDAFSXXXXXXXXXQKPTNMNGARKLPCIPLEEVTLEYLQKISTLGCGSSGKVYMVKHVKTRKIYALRxxEEKHELAVRNKIMREMEILLxxxxxxxxxxxxxxxxRGEISFVLDYMEGGTLAQVLQAHKKIPEHYLAEVARQVLKGLHYLHQNKIVHRDIKPSNLLINKRREVKIADFGVSTVLAHTLAQCNSFVGTCAYMSPERFDPDGYGGKYDGCSADIWSLGLSLLECALGRFPCLSPGQRPDWPTLMVAICLGXXXXXXXXXXPEFQSFIRCCLQKDALLRHTAHRLLSHPFLKKYEQQSCDLAPLLQSLHL
D5AD68_______________SSRERKAKVGLKLPIPVREDAFSKPMPLPLPLPKPPNMNGACKLPCVPLEEVTLEDLQKISTLGCGSSGKVYKVKHVKTGKIYALKxxQEKHELAVRKQIMREMEILRxxxxxxxxxxxxxxxxGGEISFVLEYMDGGTLAQVLQAHKKIPEHYLAEVARQVLKGLLYLHQNKIVHRDIKPSNLLINKRREVKIADFGVSTVLAHTLAQCNSFVGTCAYMSPERFDPDGYGGKYDGCSADIWSLGLSLLECALGRFPCLSPGQRPDWPTLMVAICLGDPPSPPPDASPEFQSFIRCCLQKDALLRHTAHGLLLHPFLKKYEQRSCDLSPLLQSLHL

SNPs (tot: 47)

Position	A %	C %	G %	T %	Probability
1338			33	66	0.997452
1440			50	50	1.0
1453	80		20		0.995493
1502	83			16	0.994512
1503	83		16		0.994512
1506	83		16		0.994512
1508		16		83	0.994512
1510		83	16		0.994512
1512	83	16			0.994512
1515			16	83	0.994512
1517		83		16	0.994512
1518	83	16			0.994512
1521		83	16		0.994512
1523	50	16	33		1.0
1524			16	83	0.994512
1676		70		30	1.0
1709	11		88		0.923564
1724	87		12		0.992554
1841	90	10			0.90298
1842	90	10			0.90298
1843		10	90		0.90298
1844	90	10			0.90298
1861			88	11	0.923564
1863	77	22			0.999999
1865			22	77	0.999999
1867		22		77	0.999999
1873	77	22			0.999999
1875		22	77		0.999999
1876			22	77	0.999999
1879		77	22		0.999999
1916	85	14			0.993533
1917		14		85	0.993533
1918		14	85		0.993533
1920			85	14	0.993533
1923	16	83			0.994512
1942		80	20		0.995493
2007	75	25			0.996473
2008		25	75		0.996473
2049	33			66	0.997452
2050	33		66		0.997452
2053	66	33			0.997452
2054		33	66		0.997452
2055		66		33	0.997452
2056	33	66			0.997452
2065		66	33		0.997452
2067	66			33	0.997452
2069	33		66		0.997452

Back

Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain