SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene26251

Length: 2378 nt

Origin of reads: Biogeco_1 Biogeco_2 Uagpf Uma

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>sp_v1.1_unigene26251
CGTCGTGACCTTGGCTATCACTCAACATATATACCTCCACTGCCAGGTGTACCCTCCTGTGCCCTCGGATGATTCCCTTGGTGATTAAACTCTGTAGCGTCGGATGATGCCTGTTAGGGTTTCTCAGTTTTACTTACTATTACTTCGGCCTCGATCTCATAGCTGAGGTTACTTTGCCCAGATCTGATAGCTGAGGCACGTACCATTACTTCACCCCGGATCTCATGGCTGAGGTTACTTCGCCCAGATCTCATAGCTGAGGCACAACAAAGTTAGGTTGGGGTAGATCAGGTACTTATTAGATTTCATCATTTCTGTAATTCTTAACACAGAATTGCTCTATTCCAGGTACTTTCTCAATAAAGAACTAGATTTCCCAACTAGTGGTATCGAACTGGCAGATGCAGGAATGAATCTCCACACCTCAATCTCTCCTGCCTGCAGCTCCAATTCGATATTATTCGTTTGACCGTTGATACGGTGGTTTGCTTCTCACATGGGATGCTGGGTTTTTCATTATTCCTTTTCAAAGTGCACTTTCTGTAAATTAGCTATACGTGTTTTCTATGACTGGTATGACAATTCTCTTTCATGGCCAGAACTCACAAGGCCTTTATTGCTCTGGCCAGGTAATTGGTTACATTTGGTATTCTTTATCGTTTCCCTGTAATTATACTTTTATGTTTTTCTAGAAACCCTCGGTTTCATTGACACAGCCTTCGTATGCTTTGTTTTGTTTAGTTAAATCATCAGTGGCTGGTGTCAAATCATCAATGACTGGTGACATCTTTTTTCATAACCTCTGCCACATACAACTACTCGTAATTTCTTTCTAGGTTCCTCTTTCTCCTTTGAACTGCTCGAAGAATTTAATGGGTTTACTCAGCATTTGACCTCTGCCACATACAACTACTCGTAATTTCTTTCTAGGTTCCTCTTTCTCCTTTGAACTGCTCGAAGAATTTAATGGGTTTACTCAGCATTTGGGTTATTGTGTGCTTTTCTTAATTGTATTCATGGTTGAATGGAGTTCGTATCAGGGTTTTTCTTGTGGGGAGATTCCTGAAATTTGTATCGTTTCTTGTCTGATTTAGTGTTGTGGAGTGACAGAGGGTTTTTGTCTGAAATATTGTTGTGGAGAGAAATAGGGTATAGAGAGGCAAAGGCAAACTTTCTGTTTCAGTGGGAAGACGAGTTCAAGGGAGAGAAAAACGAGGGTGGGTTTGAAGCTAGCAATCCCAGCTTGGGAAGACGCATTTTCTAAGCCAATGCAATTGCCACTTCCACTGCAGAAGCCCACCAACATGAATGGTGCTCGCAAGTTACCCTGTATTCCTCTTGAATAAGTTACACTAGAATATCTCCAGAAGATTTCAACTTTGGGGTGTGGAAGCAGTGGCAAGGTCTACAAGGTTAAGCATGCGAAGACTAGGAAAATTTATGCTTTGAGGAAAAGCACGAGCTTGCTGTTCGAAATAAAATAATGAGGGAAATGGAGATTCTCCTAAGGGCGAATTCTCCACACGTCTTGCAGTGTTATGGCATAGTCGAGCGGATTCTCCCCACATCGTGCAGTGTTATGGCATATTCGATCGAAGAGGAGAGATCTCTTTTGTGTTGGACTACATGGACGGTGGAACCCTTGCGCAGGTTCTTCAAGCCCACAAAAAATTCCCAGAACACTATTTGGCTGAGATTGCGAGGCAGGTGCTGAAGGGCATGCATTACCTACACCACAACAAAATTGTTCACCGTGATATAAAGCCCTCCAATTTACTGATCATTAAGGGAAGTCAAGATTGTCGATTTCGGTGTCAGAACTGTGTTGGTTCACACTTTGGCCCAGTGCAATGCAATTCCTTCGTGGGTGCTTATATGAGTCCTGAAAGGTTCGATCCTGATGGGTACGGTGGAAAGTATGATGGATGCTCTGCAGATATATGGAGCTTGGGATTATCTTTGTTGGAATGTGCGCTTGGAAGGTTCCCTTGTTTGTCTCCGGGGCAGAGGCCTGATTGGCCTACTTTAATGGTGGCCATCTGTTTGGGCGACCCTCCATCCCCGCCACCTGATGCATCGCCAGAGTTTCAGAGTTTTATCAGATGTTGCCTTCAAAAGGATGCATTACTTCGCCATACTGCACATCGGCTGCTTTCGCATCCTTTTTTGAAGAAGTATGAACAGCAATCTTGTGACCTGGCTCCCCTCCTGCAGTCTTTACACCTGTAGAATTTTGAATTCCTTTTTGTATTTTGAATATTGTACCTGAGAGCATTCATTGACTTGTAATGAATGTACACTCTCTTGGTCTCTGGAACTCTATTTTGTAAATCATTTTGCAATGCAACTGCAGTCTTCTTTACAGCACTTTGTCATAC

Ace file of the UniGene sp_v1.1_unigene26251 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	MKK9; MKK9 (MAP KINASE KINASE 9); MAP kinase kinase/ kinase/ protein kinase activator	-	-	47%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	82%
Blast2go	protein	-	-	69%

Source	GOs	Term	Type	Identity
Blast2go	response to wounding	GO:0009611	Biological Process	69%
Blast2go	positive regulation of cellular transcription, DNA-dependent	GO:0045941	Biological Process	69%
Blast2go	protein autophosphorylation	GO:0046777	Biological Process	69%
Blast2go	ethylene biosynthetic process	GO:0009693	Biological Process	69%
Blast2go	ethylene mediated signaling pathway	GO:0009873	Biological Process	69%
Blast2go	camalexin biosynthetic process	GO:0010120	Biological Process	69%
Blast2go	response to salt stress	GO:0009651	Biological Process	69%
Blast2go	MAP kinase kinase activity	GO:0004708	Molecular Function	69%
Blast2go	protein binding	GO:0005515	Molecular Function	69%
Blast2go	protein kinase activator activity	GO:0030295	Molecular Function	69%

Full-Lengther Next Prediction

Fln status: Putative Complete

Fln database: coniferopsida.fasta

Fln subject: D5AD68

Fln msg: Warning!, your query overlaps and the subject is separated, and overlapping frame ERROR between 1555 and 1549, W1: There is no M at the beginning,

Fln protein: GIERQRQTFCFSGKTSSRERKTRVGLKLAIPAWEDAFSKPMQLPLPLQKPTNMNGARKLPCIPLE*VTLEYLQKISTLGCGSSGKVYKVKHAKTRKIYAxxxKHELAVRNKIMREMEILLRANSPHVLQCYGxxxxxSPHIVQCYGIFDRRGEISFVLDYMDGGTLAQVLQAHKKFPEHYLAEIARQVLKGMHYLHHNKIVHRDIKPSNLLIIKGSQDCRFRCQNCVGSHFGPVQCNSFVGAYMSPERFDPDGYGGKYDGCSADIWSLGLSLLECALGRFPCLSPGQRPDWPTLMVAICLGDPPSPPPDASPEFQSFIRCCLQKDALLRHTAHRLLSHPFLKKYEQQSCDLAPLLQSLHL*

Protein Length: 362

Fln nts: CGTCGTGACCTTGGCTATCACTCAACATATATACCTCCACTGCCAGGTGTACCCTCCTGTGCCCTCGGATGATTCCCTTGGTGATTAAACTCTGTAGCGTCGGATGATGCCTGTTAGGGTTTCTCAGTTTTACTTACTATTACTTCGGCCTCGATCTCATAGCTGAGGTTACTTTGCCCAGATCTGATAGCTGAGGCACGTACCATTACTTCACCCCGGATCTCATGGCTGAGGTTACTTCGCCCAGATCTCATAGCTGAGGCACAACAAAGTTAGGTTGGGGTAGATCAGGTACTTATTAGATTTCATCATTTCTGTAATTCTTAACACAGAATTGCTCTATTCCAGGTACTTTCTCAATAAAGAACTAGATTTCCCAACTAGTGGTATCGAACTGGCAGATGCAGGAATGAATCTCCACACCTCAATCTCTCCTGCCTGCAGCTCCAATTCGATATTATTCGTTTGACCGTTGATACGGTGGTTTGCTTCTCACATGGGATGCTGGGTTTTTCATTATTCCTTTTCAAAGTGCACTTTCTGTAAATTAGCTATACGTGTTTTCTATGACTGGTATGACAATTCTCTTTCATGGCCAGAACTCACAAGGCCTTTATTGCTCTGGCCAGGTAATTGGTTACATTTGGTATTCTTTATCGTTTCCCTGTAATTATACTTTTATGTTTTTCTAGAAACCCTCGGTTTCATTGACACAGCCTTCGTATGCTTTGTTTTGTTTAGTTAAATCATCAGTGGCTGGTGTCAAATCATCAATGACTGGTGACATCTTTTTTCATAACCTCTGCCACATACAACTACTCGTAATTTCTTTCTAGGTTCCTCTTTCTCCTTTGAACTGCTCGAAGAATTTAATGGGTTTACTCAGCATTTGACCTCTGCCACATACAACTACTCGTAATTTCTTTCTAGGTTCCTCTTTCTCCTTTGAACTGCTCGAAGAATTTAATGGGTTTACTCAGCATTTGGGTTATTGTGTGCTTTTCTTAATTGTATTCATGGTTGAATGGAGTTCGTATCAGGGTTTTTCTTGTGGGGAGATTCCTGAAATTTGTATCGTTTCTTGTCTGATTTAGTGTTGTGGAGTGACAGAGGGTTTTTGTCTGAAATATTGTTGTGGAGAGAAATAGGGTATAGAGAGGCAAAGGCAAACTTTCTGTTTCAGTGGGAAGACGAGTTCAAGGGAGAGAAAAACGAGGGTGGGTTTGAAGCTAGCAATCCCAGCTTGGGAAGACGCATTTTCTAAGCCAATGCAATTGCCACTTCCACTGCAGAAGCCCACCAACATGAATGGTGCTCGCAAGTTACCCTGTATTCCTCTTGAATAAGTTACACTAGAATATCTCCAGAAGATTTCAACTTTGGGGTGTGGAAGCAGTGGCAAGGTCTACAAGGTTAAGCATGCGAAGACTAGGAAAATTTATGCTxxxxxxxxxAAGCACGAGCTTGCTGTTCGAAATAAAATAATGAGGGAAATGGAGATTCTCCTAAGGGCGAATTCTCCACACGTCTTGCAGTGTTATGGCATxxxxxxxxxxxxTTCTCCCCACATCGTGCAGTGTTATGGCATATTCGATCGAAGAGGAGAGATCTCTTTTGTGTTGGACTACATGGACGGTGGAACCCTTGCGCAGGTTCTTCAAGCCCACAAAAAATTCCCAGAACACTATTTGGCTGAGATTGCGAGGCAGGTGCTGAAGGGCATGCATTACCTACACCACAACAAAATTGTTCACCGTGATATAAAGCCCTCCAATTTACTGATCATTAAGGGAAGTCAAGATTGTCGATTTCGGTGTCAGAACTGTGTTGGTTCACACTTTGGCCCAGTGCAATGCAATTCCTTCGTGGGTGCTTATATGAGTCCTGAAAGGTTCGATCCTGATGGGTACGGTGGAAAGTATGATGGATGCTCTGCAGATATATGGAGCTTGGGATTATCTTTGTTGGAATGTGCGCTTGGAAGGTTCCCTTGTTTGTCTCCGGGGCAGAGGCCTGATTGGCCTACTTTAATGGTGGCCATCTGTTTGGGCGACCCTCCATCCCCGCCACCTGATGCATCGCCAGAGTTTCAGAGTTTTATCAGATGTTGCCTTCAAAAGGATGCATTACTTCGCCATACTGCACATCGGCTGCTTTCGCATCCTTTTTTGAAGAAGTATGAACAGCAATCTTGTGACCTGGCTCCCCTCCTGCAGTCTTTACACCTGTAGAATTTTGAATTCCTTTTTGTATTTTGAATATTGTACCTGAGAGCATTCATTGACTTGTAATGAATGTACACTCTCTTGGTCTCTGGAACTCTATTTTGTAAATCATTTTGCAATGCAACTGCAGTCTTCTTTACAGCACTTTGTCATAC

Fln Alignment:

sp_v1.1_unigene26251___SSRERKTRVGLKLAIPAWEDAFSXXXXXXXXXXXPTNMNGARKLPCIPLE*VTLEYLQKISTLGCGSSGKVYKVKHAKTRKIYALRxxEEKHELAVRNKIMREMEILLxxxxxxxxxxxxxxxxRGEISFVLDYMDGGTLAQVLQAHKKFPEHYLAEIARQVLKGMHYLHHNKIVHRDIKPSNLLIIKGSQD--CRFRCQNCVGSHFGPVQCNSFVG--AYMSPERFDPDGYGGKYDGCSADIWSLGLSLLECALGRFPCLSPGQRPDWPTLMVAICLGXXXXXXXXXXPEFQSFIRCCLQKDALLRHTAHRLLSHPFLKKYEQQSCDLAPLLQSLHL
D5AD68_______________SSRERKAKVGLKLPIPVREDAFSKPMPLPLPLPKPPNMNGACKLPCVPLEEVTLEDLQKISTLGCGSSGKVYKVKHVKTGKIYALKxxQEKHELAVRKQIMREMEILRxxxxxxxxxxxxxxxxGGEISFVLEYMDGGTLAQVLQAHKKIPEHYLAEVARQVLKGLLYLHQNKIVHRDIKPSNLLINKRREVKIADFGVSTVLAHTLA--QCNSFVGTCAYMSPERFDPDGYGGKYDGCSADIWSLGLSLLECALGRFPCLSPGQRPDWPTLMVAICLGDPPSPPPDASPEFQSFIRCCLQKDALLRHTAHGLLLHPFLKKYEQRSCDLSPLLQSLHL

SNPs (tot: 103)

Position	A %	C %	G %	T %	Probability
1145		10	90		0.90298
1147		10		90	0.90298
1149			88	11	0.923564
1197		11		88	0.923564
1269		85	14		0.993533
1270	12			87	0.992554
1273		87	12		0.992554
1276			12	87	0.992554
1280		87	12		0.992554
1281	87		12		0.992554
1282	87			12	0.992554
1288	75		25		1.0
1289	87			12	0.992554
1293			87	12	0.992554
1371		16		83	0.994512
1508			16	83	0.994512
1510	83		16		0.994512
1511	83			16	0.994512
1512			16	83	0.994512
1513	16			83	0.994512
1515	16			83	0.994512
1516			16	83	0.994512
1518		14	85		0.993533
1521	14	85			0.993533
1522	85		14		0.993533
1528	83		16		0.994512
1530		16	83		0.994512
1584		11		88	0.923564
1585		12	87		0.992554
1586	87	12			0.992554
1588	87			12	0.992554
1596		14		85	0.993533
1598	85		14		0.993533
1735	83		16		0.994512
1737	83		16		0.994512
1740		16		83	0.994512
1742	85		14		0.993533
1747			14	85	0.993533
1748			12	87	0.992554
1749	87			12	0.992554
1751	12	87			0.992554
1752		12		87	0.992554
1754	12		87		0.992554
1756		87	12		0.992554
1816	83	16			0.994512
1817	83	16			0.994512
1818	83	16			0.994512
1820		83		16	0.994512
1921	80		20		0.995493
1924	80		20		0.995493
1930			20	80	0.995493
1931		20		80	0.995493
1932		20	80		0.995493
1933	20		80		0.995493
1936		80	20		0.995493
1941		20	80		0.995493
1945		20	80		0.995493
2133	9	90			1.0
2150	87		3		1.0
2173	90	9			1.0
2187		58		41	1.0
2208	51		48		1.0
2210	70	14	3	11	1.0
2211	77	11	11		1.0
2212	11	85	3		1.0
2213		80	15	3	1.0
2215		91	8		0.999807
2217		13	86		1.0
2218	9	86			0.999642
2219		9	9	81	0.999363
2220	86	13			1.0
2221		9		81	0.999363
2222	42		52		1.0
2225	80		19		1.0
2230		19	80		1.0
2232	19	80			1.0
2258	90			10	0.90298
2259	90		10		0.90298
2262	10	90			0.90298
2263		90	10		0.90298
2264		10		90	0.90298
2265	77	22			0.999999
2266	88	11			0.923564
2267	11	88			0.923564
2268	77	11	11		0.968132
2270	11		88		0.923564
2271		11		22	0.999999
2273	87	12			0.992554
2274		25		75	1.0
2275		75	25		1.0
2276	87		12		0.992554
2277		12		87	0.992554
2278		83	16		0.994512
2322		75	25		0.996473
2328		25	75		0.996473
2329	25		75		0.996473
2332	75	25			0.996473
2335		50	25		0.995533
2336	25	50	25		0.995533
2337	25		25	50	0.995533
2339			66	33	0.997452
2344	33			66	0.997452
2345		33	66		0.997452

Back

Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain