SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene26177

Length: 1514 nt

Origin of reads: Biogeco_1 Biogeco_2 Uagpf Uma

UniGene Fasta

Ace file of the UniGene sp_v1.1_unigene26177

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	long-chain-fatty-acid CoA ligase, putative (EC:6.2.1.3)	-	-	61%
FL-Next	sp=Long chain acyl-CoA synthetase 9, chloroplastic; Arabidopsis thaliana (Mouse-ear cress).	-	-	65%
Blast2go	long chain acyl- synthetase 9	-	-	78%

Source	ECs	Term	Type	e value	Identity
Blast2go	Long-chain-fatty-acid--CoA ligase.	EC:6.2.1.3	-	0.0	78%

Source	KEGGs	Term	Type	e value	Identity
Blast2go	Fatty acid metabolism	00071		0.0	78%
Blast2go	Metabolic pathways	01100		0.0	78%

Source	GOs	Term	Type	Identity
Blast2go	long-chain fatty acid-CoA ligase activity	GO:0004467	Molecular Function	78%
Blast2go	chloroplast	GO:0009507	Cellular Component	78%
Blast2go	membrane	GO:0016020	Cellular Component	78%
Blast2go	endoplasmic reticulum	GO:0005783	Cellular Component	78%

Source	InterPros	Term	Type	e value	Identity
Blast2go	AMP-dependent synthetase/ligase	IPR000873	-	0.0	78%
Blast2go	AMP-binding, conserved site	IPR020845	-	0.0	78%

Full-Lengther Next Prediction

Fln status: N-terminus

Fln database: sp_plants

Fln subject: Q9CAP8

Fln msg: Distance to subject end: 335 aas, your sequence is shorter than subject: 361 - 691

Fln protein: MNAYIVGILVPVLLSFVLRNRRKGKVYGVPVDVDGDGGFAIRHHRSSSLVETPWEGASTLADLFEQACKRHAQMRLLGTRKLISREMKVSEDGRSFEKLHLGDYEWLSYGQVLERVCNFASGLVALGHEKGERVAIFAETRAEWFIALQGCFRHNNTVVTIYASLGEEALAHSLNETEVTTVICDRKQLKKLIDTSTQLDTVKHVIYMEDEGITSEPSLSGGSSSWTVSSMSRVERLGQESPVDADLPVAADIAVIMYTSGSTGLPKGVMMTHGNIVATVAAVMTIVPGLSNKDIYLAYLPLAHILELEAEAVMVASGIAIGYGSTLTLTDTSSKIKKGTKGDASMLGPTLMTAVPAILDR

Protein Length: 362

Fln nts: ATGGGGGACGCTTCCTTCTTCGATAAATAGATGATTAAAAATAAATGACGTTTTATATGAAACATGACTTTAATTAAAGAAAGGAAAATGAAAGGGGGGCCTTGGCACAAACATCGTAAAATCCGCCCTGTAAATGCTGCATAGCTAACCTGACACTGCTTCGCGCATGTGTGGTATGCATTCTTAGGGTTTGTTTATTCTTCATTCACGTGTTCTGTGAATTCTCTGTAGAGGTATGTTAGCCTCCTTCACCTGGCCGATGAGTCCCGCTACTGAAGATCAGTTGCTTTCGATGAGCAAGGTTTTAAATTTTGCTTAAGTTTCTTAAGTTACTGGCCAGATGCAAGCGACCCAACTGTGCCCTTGTTGCAGTCATCATTAAAAGCAGATTCTCTGGCATCTGTCCAGCAATAAACCCTGTTCACAAAG_-_ATGAATGCCTACATTGTTGGCATCCTTGTGCCTGTGCTGCTATCATTCGTGTTAAGGAACAGAAGAAAGGGAAAGGTATATGGAGTGCCTGTGGATGTGGACGGGGATGGTGGTTTTGCAATCCGACACCATCGCAGTTCTTCCTTAGTTGAAACGCCGTGGGAAGGAGCTTCAACTCTTGCGGACCTCTTTGAGCAAGCATGCAAAAGACATGCACAAATGCGCTTACTCGGAACCCGAAAGCTGATTAGCAGAGAAATGAAAGTCAGTGAAGATGGCAGGAGTTTTGAAAAGCTACATCTGGGAGATTATGAGTGGTTAAGTTATGGGCAAGTACTCGAGCGTGTTTGTAACTTTGCATCAGGTTTGGTAGCACTTGGTCATGAAAAAGGAGAGCGTGTTGCCATTTTTGCTGAAACTCGAGCAGAATGGTTCATTGCCTTGCAAGGTTGCTTCCGTCACAACAACACAGTAGTTACGATTTATGCTTCTTTGGGAGAGGAAGCTCTTGCTCACTCACTAAATGAGACAGAAGTGACAACAGTGATTTGTGATCGCAAGCAGTTAAAAAAGCTGATTGATACAAGCACACAATTGGATACTGTAAAGCATGTTATTTACATGGAAGACGAGGGCATTACATCCGAGCCATCTCTGTCTGGAGGGAGCAGCAGCTGGACAGTGTCATCTATGTCCAGGGTTGAGCGTTTGGGTCAGGAGAGTCCTGTTGATGCAGATTTGCCGGTAGCTGCTGACATTGCAGTTATAATGTATACAAGTGGCAGTACTGGCTTGCCTAAGGGAGTTATGATGACTCATGGAAATATTGTAGCGACAGTTGCCGCCGTGATGACAATTGTTCCAGGCCTTAGTAACAAAGACATATATCTGGCATACTTGCCGTTAGCTCATATTCTTGAACTAGAAGCTGAGGCTGTAATGGTTGCTTCAGGGATTGCTATTGGTTATGGTTCAACTTTGACACTAACTGATACATCAAGCAAGATTAAAAAAGGAACCAAAGGAGATGCTTCTATGCTGGGTCCAACATTGATGACTGCTGTACCGGCTATTTTAGATCGTGT

Fln Alignment:

sp_v1.1_unigene26177___MNAYIVGILVPVLLSFVLRNRRKGKXXXXXXXXXXXXXFAIRHHRSSSLVETPWEGASTLADLFEQACKRHAQMRLLGTRKLISREMKVSEDGRSFEKLHLGDYEWLSYGQVLERVCNFASGLVALGHEKGERVAIFAETRAEWFIALQGCFRHNNTVVTIYASLGEEALAHSLNETEVTTVICDRKQLKKLIDTSTQLDTVKHVIYMEDEGITXXXXXXXXXXXXXXXXXXRVERLGQESPVDADLPVAADIAVIMYTSGSTGLPKGVMMTHGNXXXXXXXXXXXXPGLSNKDIYLAYLPLAHILELEAEAVMVASGIAIGYGSTLTLTDTSSKIKKGTKGDASMLGPTLMTAVPAILDR
Q9CAP8_______________MIPYAAGVIVPLALTFLVQKSKKEKKRGVVVDVGGEPGYAIRNHRFTEPVSSHWEHISTLPELFEISCNAHSDRVFLGTRKLISREIETSEDGKTFEKLHLGDYEWLTFGKTLEAVCDFASGLVQIGHKTEERVAIFADTREEWFISLQGCFRRNVTVVTIYSSLGEEALCHSLNETEVTTVICGSKELKKLMDISQQLETVKRVICMDDE------FPSDVNSNWMATSFTDVQKLGRENPVDPNFPLSADVAVIMYTSGSTGLPKGVMMTHGNVLATVSAVMTIVPDLGKRDIYMAYLPLAHILELAAESVMATIGSAIGYGSPLTLTDTSNKIKKGTKGDVTALKPTIMTAVPAILDR

SNPs (tot: 31)

Position	A %	C %	G %	T %	Probability
160	8		25	66	1.0
164		66	8	25	1.0
166	27	9			1.0
167		27	9		1.0
169	75			25	1.0
172			27	72	1.0
173		27	72		1.0
176			72	27	1.0
177		27		72	1.0
180		27	72		1.0
181	27	72			1.0
201		80		20	0.999996
280			88	11	0.923564
351	75		25		1.0
531	33		66		1.0
532	33	66			1.0
533		33	66		1.0
561		83		16	0.994512
562	83			16	0.994512
570			16	83	0.994512
571	16	83			0.994512
572			16	83	0.994512
575		83	16		0.994512
576	16			83	0.994512
577		16		83	0.994512
580			16	83	0.994512
581		16		83	0.994512
582		16	83		0.994512
630			25	75	1.0
1439		10		90	0.90298
1470		50		50	1.0

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain