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Fln status: Internal

Fln database: tr_plants

Fln subject: B9H949

Fln msg: Overlapping hits, possible frame ERROR between 63 and 59, and possible frame ERROR between 1449 and 1502, Unexpected STOP codon at 3' end. Distance to subject end: 166 aas, your sequence is shorter than subject: 557 - 1334

Test code: 0

Test Code was used to find complete genes when there was not found a reliable orthologue. The best ORF (Open Reading Frame) is shown, and only ORFs > 200pb were analyzed. Your ORF will be more reliable if a stop codon was found before the start codon. A Test Code value > 0.95 means the ORF is probably coding. A Test Code value < 0.74 means the ORF is probably non-coding. Test Code values in between 0.74 and 0.95 mean it is uncertain whether the ORF is coding or not.

Fln protein: LAFSQEKSIYEAVEGAFIQxxxKKNSMETAANLINITIDASIGDLAALEHIITALVTKGDITSGTISALWDFFTFNVNGVTPEQSRGALSVLCMAAKSSPKVLSSRLLNIIDIGFGRWAKEEPLLARTACIALQRLSVEDRDSLFSVNRRVFNVLQSLIIGPGLPEQSWYAASEQAINAIYALHPMPEMFLSDVIRKFHGSVFGQNEALEQLVSHVEKRKMRHLFSMTLYLFMLQSSVDTCLL*VM*P*NTWFI*NHVYGRFESRKRTKKEQLPSHRLRNLMTLMPRNLLTKHQRRTISMLSWAFLHPKMQGLIYLLRKPRRKSFREDKIKNL*LEYVHLLLQSSVETLRCCNSFQDCRHLLCWLFAS*WLLMQNSVMQICSFSSQWPKMLSQNLFAQIVP*LWEIWQCVFLIFWILGLRICMHALMIRLYP*EKMQC*FCHTSY*MT**RSKVT*VRWLFI*RMKTCGSLIL*SSFSMNCQxxxxxxxxxxxxxxxxxxxSKTYQRRCSVILCRS*LVLSRRTNKWKGLSRSSVIDFLESQIKSNGLTLLIAFLN*

Protein Length: 558

Fln nts: TTGGCCTTCTCTCAAGAGAAATCCATCTATGAGGCTGTCGAAGGTGCATTCATTCAAxxxxxxxxxAAGAAGAACTCCATGGAAACTGCAGCCAATTTGATAAACATCACAATAGATGCTAGCATTGGAGATCTTGCAGCCTTGGAACATATAATCACTGCATTGGTCACCAAAGGAGACATTACTTCTGGAACGATATCTGCTCTTTGGGATTTTTTTACCTTCAATGTCAATGGAGTAACACCAGAGCAAAGCCGTGGAGCTTTATCAGTGCTATGCATGGCTGCAAAGTCTTCCCCAAAGGTCCTCAGTTCACGTTTGTTAAATATTATTGATATTGGATTTGGTCGTTGGGCAAAGGAGGAACCCTTACTTGCAAGGACAGCTTGTATTGCTCTTCAACGACTCTCAGTAGAAGACCGAGACAGTCTTTTCTCTGTTAATCGCCGAGTTTTTAATGTCTTACAAAGTTTGATTATTGGTCCGGGGCTTCCAGAACAAAGCTGGTATGCTGCTTCTGAGCAGGCCATTAATGCTATCTATGCGCTTCATCCCATGCCAGAAATGTTTCTGTCTGATGTCATAAGAAAATTTCATGGCAGTGTTTTTGGGCAAAATGAAGCACTAGAACAACTGGTTTCACATGTTGAAAAGAGGAAGATGCGGCATCTCTTCTCAATGACCCTCTATCTGTTCATGTTACAAAGCTCAGTAGATACATGTTTGTTGTGAGTCATGTAGCCATGAAACACTTGGTTTATATAGAATCATGTGTACGGAAGGTTCGAAAGCAGAAAGCGGACAAAGAAAGAGCAGCTGCCGAGTCACAGGCTGCGAAACTTGATGACATTGATGCCACGCAATCTTCTGACAAAGCATCAGAGGAGAACAATATCAATGCTGAGTTGGGCGTTTCTGCATCCGAAGATGCAAGGCTTGATTTACTTGCTGAGAAAGCCGAGAAGGAAATCGTTTCGGGAAGACAAAATCAAAAATTTATAATTGGAGTATGTGCACCTGTTATTGCAAAGCTCTGTAGAAACTTTACGCTGCTGCAACAGTTTCCAGGACTGCAGGCATCTGCTATGCTGGCTCTTTGCAAGCTAATGGTTGTTGATGCAGAATTCTGTGATGCAAATCTGCAGCTTCTCTTCACAGTGGCCCAAAATGCTGTCTCAGAATCTGTTCGCTCAAATTGTACCATAGCTCTGGGAGATCTGGCAGTGCGTTTTCCTAATCTTCTGGATCCTTGGACTGAGAATATGTATGCACGCCTTAATGATCAGGCTTTATCCGTGAGAAAAAATGCAGTGCTAGTTTTGTCACACCTCATATTAAATGACATGATGAAGGTCAAAGGTTACATAAGTGAGATGGCTCTTCATCTAGAGGATGAAGACTTGCGGATCTCTAATCTTGTGAAGCTCTTTTTCCATGAATTGTCAAAGxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxTAGCAAAACATATCAAAGGAGATGTTCTGTAATATTATGCAGGTCCTGATTGGTTCTATCAAGAAGGACAAACAAATGGAAGGGCTTATCGAGAAGCTCTGTCATCGATTTTCTGGAATCACAGATAAAAAGCAATGGGTTGACATTGCTTATTGCCTTTCTCAACTGACCTACACCGAAAAAGGATATAAGAAACTAGCAGACTTGTTCAAAA

Fln Alignment:

sp_v1.1_unigene26155___LAFSQEKSIYEAVEGAFIQLxIKKNSMETAANLINITIDASIGDLAALEHIITALVTKGDITSGTISALWDFFTFNVNGVTPEQSRGALSVLCMAAKSSPKVLSSRLLNIIDIGFGRWAKEEPLLARTACIALQRLSVEDRDSLFSVN-RRVFNVLQSLIIGPGLPEQSWYAASEQAINAIYALHPMPEMFLSDVIRKFHGSVFxxxxxxxxxxxxxxxxxxQNISKEMFCNIMQVLIGSIKKDKQMEGLIEKLCHRFSGITDKKQWVDIAYCLSQLTYTEKGYKKLADLFK
B9H949_______________LVFSQDKSIYEAVENAFITIxVRKNPLDTAKNLLDLAIDSNIGDLAALEFIVNALVSKGDISTSTISALWDFFCFNISGTTPEQSRGALSVLCMAAKASPGVLGSHLQDIIDIGFGRWAKVDPLLARTACIAIQRLSEEDKKKLLASNGSRVFGFLENLISGSWLPENTWYAAADKAIGVIYTIHPTPETLAADLVKKSLSSVFxxxxxxxxxxxxxxxxxxQELKRETFCNIMQFLIGSIKKDKQMESLVEKLCNRFSGVIDTRQWEYISYCLSQLAFTEKGMKKLIDSFK